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Laboratory technique in biochemistry
Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular
Immunoprecipitation
Genomic technique
Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and
Chromatin_immunoprecipitation
RIP-chip (RNA immunoprecipitation chip) is a molecular biology technique which combines RNA immunoprecipitation with a microarray. The purpose of this
RNA_immunoprecipitation_chip
Purification technique
Methylated DNA immunoprecipitation (MeDIP or mDIP) is a large-scale (chromosome- or genome-wide) purification technique in molecular biology that is used
Methylated DNA immunoprecipitation
Methylated_DNA_immunoprecipitation
Branch of proteomics
substrate of any tyrosine kinase. Only by digesting the samples before immunoprecipitation can isolation of only phosphoproteins and temporal profiles of individual
Phosphoproteomics
Proximity ligation assisted chip-seq technology
Proximity ligation-assisted chromatin immunoprecipitation sequencing (PLAC-seq) is a chromatin conformation capture(3C)-based technique to detect and
PLAC-Seq
Branch of medicine studying the immune system
disease Hypersensitivity Immune disorder Immunodeficiency Significant tests Agglutination Immunoassay Immunoprecipitation Serology Specialist Immunologist
Immunology
Method used in molecular biology
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order
Cross-linking immunoprecipitation
Cross-linking_immunoprecipitation
Method used to analyze protein interactions with DNA
analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding
ChIP_sequencing
Technique in molecular biology
is a technique in molecular biology that incorporates chromatin immunoprecipitation (ChIP)-based enrichment, chromatin proximity ligation, Paired-End
ChIA-PET
Inherited retrovirus encoded in an organism's genome
the p53 pathway, which results in cell apoptosis. Using chromatin immunoprecipitation with sequencing, thirty-percent of all p53-binding sites were located
Endogenous_retrovirus
Species of fungus
Wor1 control of each gene, based on Wor1 enrichment in chromatin immunoprecipitation experiments. Activation (arrowhead) and repression (bar) are inferred
Candida_albicans
MeRIPseq (or MeRIP-seq) stands for methylated RNA immunoprecipitation sequencing, which is a method for detection of post-transcriptional RNA modifications
MeRIPseq
Species of Gram-positive bacterium
Sengupta M, Jain V, Wilkinson BJ, Jayaswal RK (June 2012). "Chromatin immunoprecipitation identifies genes under direct VraSR regulation in Staphylococcus
Staphylococcus_aureus
usually require a relatively large amount of amplified DNA. Chromatin Immunoprecipitation results in production of complex mixtures of relatively short DNA
Multiple displacement amplification
Multiple_displacement_amplification
known as BATMAN, is a statistical tool for analysing methylated DNA immunoprecipitation (MeDIP) profiles. It can be applied to large datasets generated using
Bayesian tool for methylation analysis
Bayesian_tool_for_methylation_analysis
Lab technique in molecular biology
to supplement pulse-chase analysis. These include cell staining, immunoprecipitation, and SDS-PAGE. Shortly after introduction of the labeled compound
Pulse-chase_analysis
sequence-independent but structure-specific antibody for DNA-RNA immunoprecipitation (DRIP) to capture R-loops for massively parallel DNA sequencing.
DRIP-seq
Method in molecular biology
territories were discovered. In 1984, John T. Lis innovated the Chromatin immunoprecipitation technique. In 1993, the Nuclear Ligation Assay was published, a method
Chromosome conformation capture
Chromosome_conformation_capture
Protein complex facilitating transcription
the H3/H4 tetramer (see: Nucleosome core particle) or Pol II. Co-immunoprecipitation assays with tagged recombinant proteins showed that the Spt16 subunit
FACT_(biology)
Surface glycoprotein
protein-protein interactions or to detect protein expression, using Co-Immunoprecipitation or Western blot respectively. The HA-tag is not suitable for detection
HA-tag
Protein-coding gene in the species Homo sapiens
inhibition was no longer seen. Completing binding assays and co-immunoprecipitations revealed that p75 and NgR were not bound to each other through the
Reticulon_4_receptor
Loose connective tissue composed mostly by adipocytes
as a potential therapeutic molecule to induce beiging. Chromatin immunoprecipitation with sequencing (ChIP-seq) is a method used to identify protein binding
Adipose_tissue
Measure the amount of a target entity
Chromatin immunoprecipitation ChIP-exo ChIP-on-chip ChIP-seq CUT&RUN CUT&TAG Chromosome conformation capture ChIA-PET (also chromatin immunoprecipitation) HiChIP
Assay
Chemical process
employed. Analogously, in medicine, coprecipitation (referred to as immunoprecipitation) is specifically "an assay designed to purify a single antigen from
Coprecipitation
Chemical compound
also used to crosslink proteins to DNA, as used in ChIP (chromatin immunoprecipitation) which is a technique to determine which part of DNA certain proteins
Paraformaldehyde
Molecular biology method
(also known as ChIP-chip) is a technology that combines chromatin immunoprecipitation ('ChIP') with DNA microarray ("chip"). Like regular ChIP, ChIP-on-chip
ChIP-on-chip
Phage immunoprecipitation sequencing (PhIP-Seq) is method that combines barcoded DNA high-throughput sequencing and proteomics to determine the levels
PhIP-Seq
American genetics researcher
"Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing". Nature Methods. 4 (8): 651–657
Michael_P._Snyder
Topics referred to by the same term
County, Romania IP Casino Resort Spa, in Biloxi, Mississippi, US Immunoprecipitation, a molecular biology technique Incontinentia pigmenti, a genetic
IP
Biochemical analysis procedure
angle position detector. The liquid-phase ligand binding assay of immunoprecipitation (IP) is a method that is used to purify or enrich a specific protein
Ligand_binding_assay
Protein domain
with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM. The gene for protein L contains five components:
Protein_L
Method for producing lots of identical antibodies
immunocytochemical, western blot, and immunoprecipitation-mass spectrometry. Unlike western blot assays, immunoprecipitation-mass spectrometry facilitates screening
Hybridoma_technology
Particular peptide protein tag
Examples are cellular localization studies by immunofluorescence, immunoprecipitation or detection by SDS PAGE protein electrophoresis and Western blotting
FLAG-tag
Bioinformatics database
regulatory elements (RNA-binding proteins) built from cross-linking and immunoprecipitation data. Khorshid, Mohsen; Rodak Christoph; Zavolan Mihaela (Jan 2011)
CLIPZ
Highly sensitive assay for prion detection
B, Raeber AJ, Caughey B (2011). "Prion disease blood test using immunoprecipitation and improved quaking-induced conversion". mBio. 2 (3) e00078-11:
Real-time quaking-induced conversion
Real-time_quaking-induced_conversion
Spike arrangement on stegosaur tails
interrogation of RNA-protein binding events in CLIP-Seq (Cross-linking and immunoprecipitation) data. Dinosaurs portal Tail club Timeline of stegosaur research
Thagomizer
Electrophoretic hydrolytic enzyme detection technique
Hempelmann, E.; Putfarken, B.; Rangachari, K.; Wilson, R.J.M. (1986). "Immunoprecipitation of malarial acid endopeptidase". Parasitology. 92 (2): 305–312. doi:10
Zymography
Protein that initiates male sex determination in therian mammals
identifying the genes that transcription factors act on using chromatin immunoprecipitation. One of the knockout models for the SRY gene was done in pigs. Through
Sex-determining region Y protein
Sex-determining_region_Y_protein
Professor of Molecular Genetics and Microbiology
mRNAs encoding proteins. Keene introduced the RIP (ribonucleoprotein immunoprecipitation) protocol for isolating specific mRNPs, which has become a tool for
Jack_D._Keene
Lysis buffer
buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). This buffer is more denaturing than NP-40 or Triton
Radioimmunoprecipitation assay buffer
Radioimmunoprecipitation_assay_buffer
Protein(s) forming a major part of an organism's immune system
proteins. They are also used in immunoprecipitation to separate proteins and anything bound to them (co-immunoprecipitation) from other molecules in a cell
Antibody
Surface protein in bacteria cell walls
to a porous bead for purification of IgG was published in 1972. Immunoprecipitation studies with protein A conjugated to beads are also commonly used
Protein_A
Molecular biology technique
Landscape by Micrococcal Nuclease-Sequencing (MNase-seq)". Chromatin Immunoprecipitation. Methods in Molecular Biology. Vol. 1689. pp. 83–101. doi:10
ATAC-seq
Process of extracting design information from anything artificial
specific gene, a regulatory connection can be hypothesized. Chromatin ImmunoPrecipitation (ChIP) methods investigate the genome-wide profile of DNA binding
Reverse_engineering
Protein-coding gene in the species Homo sapiens
Zhang T, Zhang MQ, Farnham PJ (October 2001). "Use of chromatin immunoprecipitation to clone novel E2F target promoters". Molecular and Cellular Biology
BECN1
Protein in the extracellular matrix
and its regulation during EMT were confirmed by ribonucleoprotein immunoprecipitation. La is able to positively modulate LamB1 IRES translation, so LamB1
Laminin
transcription start sites (TSS). cfMeDIP-seq (cell-free methylated DNA immunoprecipitation and high-throughput sequencing) is a fragment-based method used to
Fragmentomics
Protein found in humans
Genome-wide analysis of HMGA2 target genes was performed by chromatin immunoprecipitation in a gastric cell line with overexpressed HMGA2, and 1,366 genes
HMGA2
Collection of microscopic DNA spots attached to a solid surface
detection, mostly combining PCR and microarray technology. Chromatin immunoprecipitation on Chip DNA sequences bound to a particular protein can be isolated
DNA_microarray
is possible as quantitative reads can also be achieved. Chromatin immunoprecipitation (ChIP) is a method for determining transcription factor binding sites
ABI_Solid_Sequencing
American biotechnology company
Reagents are created for use in flow cytometry, proteogenomics, ELISA, immunoprecipitation, Western blotting, immunofluorescence microscopy, immunohistochemistry
BioLegend
ChIP-exo is a chromatin immunoprecipitation based method for mapping the locations at which a protein of interest (transcription factor) binds to the
ChIP-exo
Method of blood examination
533. PMC 500564. PMID 8813948. Hempelmann E, Wilson RJ (1982). "Immunoprecipitation of malarial enzymes". Protozoology. 29: 637. Wikimedia Commons has
Blood_smear
Antibodies from clones of the same blood cell
purify their target compounds from mixtures, using the method of immunoprecipitation.[citation needed] Therapeutic monoclonal antibodies act through multiple
Monoclonal_antibody
Transformation of a stem cell to a more specialized cell
genomic binding site or not. This can be determined using a chromatin immunoprecipitation assay. DNA-nucleosome interactions are characterized by two states:
Cellular_differentiation
Gene and protein involved in Huntington's disease
were typically found using two-hybrid screening and confirmed using immunoprecipitation. Huntingtin has also been shown to interact with: UBE2K, MAP3K10
Huntingtin
RNA family
2'-O-methylation of substrate RNAs. snoRNA U63 was purified from HeLa cells by immunoprecipitation with antifibrillarin antibody. It is predicted to guide the 2'-O-ribose
Small_nucleolar_RNA_SNORD63
Artificial peptide attached to protein for marking purpose
particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification
Protein_tag
Molecular structure formed in DNA transcription
M. H.; Allis, C. D. (November 1999). "In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA associations in a chromatin environment"
Transcription_bubble
Chemical compound
Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation 'Fix' protein interactions to allow identification of weak or transient
Disuccinimidyl_suberate
Biological process
the HELP assay. Methylated DNA immunoprecipitation (MeDIP), analogous to chromatin immunoprecipitation, immunoprecipitation is used to isolate methylated
DNA_methylation
Protein-RNA binding probing method
crosslinking immunoprecipitation or CLIP-seq) PAR-CLIP (Photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation) iCLIP (Individual
Ribonucleoprotein Networks Analyzed by Mutational Profiling
Ribonucleoprotein_Networks_Analyzed_by_Mutational_Profiling
Family of heat shock proteins
Hsp70 and acts as a ubiquitin ligase for clients. CHIP, chromatin immunoprecipitation; Hsp70, heat shock protein 70 kDa; TPR, tetratricopeptide repeat
Hsp70
Institute and Harvard Medical School. Technologies such as chromatin immunoprecipitation combined with microarray analysis "ChIP-on-chip" or with massively
Cistrome
Protein that regulates the rate of DNA transcription
for identifying transcription factor binding sites is chromatin immunoprecipitation (ChIP). This technique relies on chemical fixation of chromatin with
Transcription_factor
Cell-cycle regulatory complex
binding to the D-box of CLB2. Based upon hybrid assays in vivo and co-immunoprecipitation in vitro, it is suggested that Cdc16p, Cdc23p and Cdc27p (analogs
Anaphase-promoting_complex
Control of gene expression at the RNA level, between transcription and translation
post-transcriptional regulation several techniques are used, such as RIP-Chip (RNA immunoprecipitation on chip). Deficiency of expression of a DNA repair gene occurs in
Post-transcriptional regulation
Post-transcriptional_regulation
Protein family
Institutes of Health in 1984. Using the techniques of radioiodination and immunoprecipitation, they found a unique but yet unknown antigen from P. falciparum-infected
Plasmodium falciparum erythrocyte membrane protein 1
Plasmodium_falciparum_erythrocyte_membrane_protein_1
Topics referred to by the same term
Corticotropin-like intermediate peptide, an endogenous neuropeptide Cross-linking immunoprecipitation, a method used in molecular biology to locate RNA modifications among
Clip
Anti-nuclear antibody
(Western blot), counterimmunoelectrophoresis (CIE), and immunoprecipitation (IP). While immunoprecipitation is considered the reference standard due to its high
Anti-Jo1
Field of study
detected on a genome wide level through the coupling of chromatin immunoprecipitation (ChIP) technology with DNA microarrays, termed ChIP-Chip. However
Epigenomics
RNA family
was one of seventeen snoRNAs identified in Trypanosoma brucei by immunoprecipitation with anti-fibrillarin antibodies. Together with TBR4, TBR6 and TBR2
Small_nucleolar_RNA_TBR12
Medical condition
the National Institutes of Health have described the a luciferase immunoprecipitation assay (LIPS) and the related QLIPS (quick version). Whereas a previously
Loa_loa_filariasis
Protein found in humans
been shown to interact with Ubiquitin C via both protein complex immunoprecipitation and affinity chromatography. Two-hybrid screening assays have shown
NBPF1
Species of fungus
vitro growth in hypoxia as well as attenuated virulence. Chromatin immunoprecipitation studies with the SrbA protein led to the identification of a second
Aspergillus_fumigatus
Novel genomic technique
Other variations, such as ChIP-loop and ChIA-PET, combine 3C with immunoprecipitation assays to detect interactions mediated by a protein of interest.
Pore-C
Chemical compound
to stabilize protein-protein interactions for further analysis by immunoprecipitation or crosslinking mass spectrometry. The deuterated crosslinker
Bissulfosuccinimidyl_suberate
Method used to analyze protein interactions with DNA
interest is required. Sono-Seq: Identical to ChIP-Seq but without the immunoprecipitation step. HITS-CLIP: Also called CLIP-Seq, employed to detect interactions
CUT&RUN_sequencing
Protein-coding gene in the species Homo sapiens
function. α-dystroglycan was shown to interact with pikachurin through immunoprecipitation. Dystroglycan ligand with other proteins is essential. Glycosylation
Pikachurin
Physical interactions and constructions between multiple proteins
been emerging along with technology progression. These include co-immunoprecipitation, protein microarrays, analytical ultracentrifugation, light scattering
Protein–protein_interaction
Type of stable macromolecular complex
commonly used for identifying the complexes[clarification needed] is immunoprecipitation. Recently, Raicu and coworkers developed a method to determine the
Protein_complex
Buffer to break cells in molecular biology
Adjust pH to 7.4 RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer
Lysis_buffer
Type of RNA found in cells
high-throughput sequencing of RNA isolated by cross-linking and immunoprecipitation (HITS-CLIP) data . CDR1as/CiRS-7 is encoded in the genome antisense
Circular_RNA
specificity of this protein is then typically confirmed using Chromatin Immunoprecipitation (ChIP) and Sanger sequencing of the resulting DNA fragment. This
Epigenome_editing
Method used to detect DNA damage
detect genome-wide double-strand DNA damage. In contrast to chromatin immunoprecipitation (ChIP)-based methods of identifying DNA double-strand breaks (DSBs)
BLESS
Protein-coding gene in the species Homo sapiens
study of interferon alpha signaling pathway using a combination of immunoprecipitation and immobilized metal affinity chromatography". Mol. Cell. Proteomics
BCLAF1
Swiss biochemist and physiologist (1877–1950)
experiments indeed seemed to "work" on occasion was probably due to immunoprecipitation. The crucial difference between this and Abderhalden's theory is
Emil_Abderhalden
Protein-coding gene in humans
utilization of mouse genetics, gene expression analyses, chromatin immunoprecipitation studies and luciferase reporter assays, it has been shown that IRF6
IRF6
Complex of DNA and protein in eukaryotic cells
to its pre-damage level after about 20 min. ChIP-seq (Chromatin immunoprecipitation sequencing) is recognized as the vastly utilized chromatin identification
Chromatin
Protein complex
make up the MuvB complex. Immunoprecipitation of MuvB factors also revealed association of BMYB. Subsequent immunoprecipitation with BMYB yielded all the
DREAM_complex
role in regulating gene expression and DNA replication. chromatin immunoprecipitation (ChIP) chromocenter A central amorphous mass of polytene chromosomes
Glossary of cellular and molecular biology (0–L)
Glossary_of_cellular_and_molecular_biology_(0–L)
BLOC-1 demonstrates physical association with AP-3 and BLOC-2 upon immunoprecipitation, although not to both complexes at the same time. Indeed, BLOC-1
Biogenesis of lysosome-related organelles complex 1
Biogenesis_of_lysosome-related_organelles_complex_1
Cell cycle checkpoint
close to the centromeres, as it was shown in a study using chromatin immunoprecipitation. Classical cytologic observations suggested that sister chromatids
Spindle_checkpoint
Chemical compound
it was noted as the best detergent for improving selectivity of immunoprecipitation of phosphotyrosine modified proteins. This detergent has also been
Octyl_glucoside
Biochemical purification technique
Tandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions. The goal is to extract
Tandem_affinity_purification
Peak finding software
specific protein binding to a control sample (e.g., input DNA or IgG immunoprecipitation), if available, to distinguish genuine enrichment from background
MACS_(software)
Serotonin receptor protein distributed in the cerebrum and raphe nucleus
"Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation". The Journal of Biological Chemistry. 277 (18): 15482–15485. doi:10
5-HT1A_receptor
Histone methylation on tail of histone H3 associated with gene bodies
mark H3K36me3 can be detected in a variety of ways: 1. Chromatin Immunoprecipitation Sequencing (ChIP-sequencing) measures the amount of DNA enrichment
H3K36me3
protocol, since it follows the same steps except it doesn't require immunoprecipitation. Auerbach, R. K.; Euskirchen, G.; Rozowsky, J.; Lamarre-Vincent,
Sono-Seq
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