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Optical microscope that uses fluorescence and phosphorescence
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to scattering, reflection, and attenuation or absorption
Fluorescence_microscope
Optical imaging technique
limitations of traditional wide-field fluorescence microscopes. In a conventional (i.e., wide-field) fluorescence microscope, the entire specimen is flooded
Confocal_microscopy
Scientific instrument for observing small objects
of microscopes are the fluorescence microscope, electron microscope (both the transmission electron microscope and the scanning electron microscope) and
Microscope
Microscope observing a thin depth of a cell
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers
Total internal reflection fluorescence microscope
Total_internal_reflection_fluorescence_microscope
Fluorescence imaging technique
single-photon excitation microscopes, which need to employ elements such as pinholes to reject out-of-focus fluorescence. The fluorescence from the sample is
Two-photon excitation microscopy
Two-photon_excitation_microscopy
(CLEM) is the combination of an optical microscope – usually a fluorescence microscope – with an electron microscope. In an integrated CLEM system, the sample
Correlative light-electron microscopy
Correlative_light-electron_microscopy
Viewing of objects which are too small to be seen with the naked eye
microscope glossary Ratio-metric Imaging Applications For Microscopes Examples of Ratiometric Imaging Work on a Microscope Interactive Fluorescence Dye
Microscopy
Measurement of number and characteristics of cells
Beckman-Coulter Inc. Retrieved 2013-03-31. Rusk, N. (2009). "The fluorescence microscope". Milestones in Light Microscopy. Nature Publishing Group. Heimstädt
Cytometry
Fluorescence microscopy technique
illumination microscope with a gated image intensifier camera has been reported that allowed measuring a map of fluorescence lifetimes (fluorescence lifetime
Light sheet fluorescence microscopy
Light_sheet_fluorescence_microscopy
Genetic testing technique
samples are visualized under a fluorescence microscope such as the confocal fluorescence microscope and the Keyence microscope. First, a probe is constructed
Fluorescence in situ hybridization
Fluorescence_in_situ_hybridization
Type of non-invasive imaging technique
induced fluorescence can allow for more accurate, up-to-date experimental data. Fluorescence in the life sciences Fluorescence microscope "Fluorescence Imaging
Fluorescence_imaging
Technology used to validate protein interactions
using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional
Bimolecular fluorescence complementation
Bimolecular_fluorescence_complementation
Type of field where the net flow of electromagnetic energy is zero
molecules for microscopy (as in the total internal reflection fluorescence microscope). The evanescent wave from an optical fiber can be used in a gas
Evanescent_field
Microscope that uses visible light
scanning laser to illuminate a sample for fluorescence. Two-photon microscope, used to image fluorescence deeper in scattering media and reduce photobleaching
Optical_microscope
Device
secondary electron images with images obtained by fluorescence microscopes. Unlike an electron microscope, FIB is inherently destructive to the specimen
Focused_ion_beam
Optical microscope used in surgery
An operating microscope or surgical microscope is an optical microscope specifically designed to be used in a surgical setting, typically to perform microsurgery
Operating_microscope
Technique in fluorescence microscopy
Confocal microscopy Fluorescence Fluorescence microscope Ground state depletion microscopy Laser scanning confocal microscopy Optical microscope Photoactivated
STED_microscopy
Type of electron microscope
A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of
Scanning_electron_microscope
Experimental technique in cell biology
causes their fluorescence lifetime to quickly elapse (limited to roughly 105 photons before extinction). Now the image in the microscope is that of a
Fluorescence recovery after photobleaching
Fluorescence_recovery_after_photobleaching
A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution. With it the typical range of the axial resolution of 500–700 nm
4Pi_microscope
Complete reflection of a wave
reflection fluorescence microscope (TIRFM), instead of relying on simple scattering, we choose an evanescent wavelength short enough to cause fluorescence (Fig
Total_internal_reflection
Thin, flat piece of glass onto which a sample is placed to be examined under a microscope
A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under
Microscope_slide
Method to evaluate biological samples
techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized. This is achieved
Comparative genomic hybridization
Comparative_genomic_hybridization
Technique used for light microscopy
emission of light in a specific predefined wavelength using a fluorescence microscope. It is imperative that the binding of the fluorophore to the antibody
Immunofluorescence
Photographic display of total chromosome complement in a cell
combinations are captured and analyzed by a fluorescence microscope using up to 7 narrow-banded fluorescence filters or, in the case of spectral karyotyping
Karyotype
Interaction of chemicals with immune responses of cells
The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated
Immunocytochemistry
Scientific investigative technique
rely on fluorescence microscopes, which use high intensity light sources, usually mercury or xenon lamps, LEDs, or lasers, to excite fluorescence in the
Fluorescence in the life sciences
Fluorescence_in_the_life_sciences
Invasion of an organism's body by pathogenic agents
bind to and identify a specific antigens present on a pathogen. A fluorescence microscope is then used to detect fluorescently labeled antibodies bound to
Infection
Artificial organism
behavior, they will glow a prespecified color when viewed under a fluorescence microscope. Xenobots can also self-replicate. Xenobots can gather loose cells
Xenobot
Photochemical energy transfer mechanism
Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is
Förster resonance energy transfer
Förster_resonance_energy_transfer
Romanian-German physicist (born 1962)
power of the fluorescence microscope, previously limited to half the wavelength of the employed light (> 200 nanometers). A microscope's resolution is
Stefan_Hell
Laboratory technique to localize nucleic acids
multiplex assays. The signal can be visualized using a fluorescence or brightfield microscope. permeabilization of cells with proteinase K to open cell
In_situ_hybridization
Eukaryotic membrane-bounded organelle containing DNA
interchromatin regions of the nucleoplasm of mammalian cells. At the fluorescence-microscope level, they appear as irregular, punctate structures, which vary
Cell_nucleus
examined in a fluorescence microscope. The F-H method allowed, for the first time, the examiner to watch these monoamines light up in the microscope and to precisely
Falck-Hillarp method of fluorescence
Falck-Hillarp_method_of_fluorescence
Technique used to enhance visual contrast of specimens observed under a microscope
electrophoresis. Light microscopes are used for viewing stained samples at high magnification, typically using bright-field or epi-fluorescence illumination. Staining
Staining
Microscopy technique
near-field scanning optical microscopes including the spatial dependence of intramolecular vibrations in anisotropic molecules. Fluorescence spectroscopy Nano-optics
Near-field scanning optical microscope
Near-field_scanning_optical_microscope
Gas discharge lamp that produces intense white light
mercury lamps are the two most common lamps used in wide-field fluorescence microscopes. Xenon arc lamps can be roughly divided into three categories:
Xenon_arc_lamp
Biological membrane structure
bilayer cannot be seen with a traditional microscope because it is too thin, so researchers often use fluorescence microscopy. A sample is excited with one
Lipid_bilayer
Chemical element with atomic number 48 (Cd)
are used for imaging of biological tissues and solutions with a fluorescence microscope. In molecular biology, cadmium is used to block voltage-dependent
Cadmium
Central Public Sector Undertaking
ion chromatograph, nitrogen analyzer, fluorescence microscope, petrography microscope, metallurgical microscope, surface area analyzer, continuous ambient
NLC_India_Limited
Scientific instruments
manipulate and image samples that exhibit fluorescence, optical tweezers can be built alongside a fluorescence microscope. Such instruments are particularly
Optical_tweezers
Study of the separation, identification, and quantification of matter
spectroscopy, ultraviolet-visible spectroscopy, X-ray spectroscopy, fluorescence spectroscopy, infrared spectroscopy, Raman spectroscopy, dual polarization
Analytical_chemistry
Spectroscopic method
which occur in the reaction zone, such as OH Fluorescence microscope Planar laser-induced fluorescence Ultrafast laser spectroscopy Wikimedia Commons
Laser-induced_fluorescence
Emission of light by a substance that has absorbed light
Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation
Fluorescence
Type of camera that can also capture the direction of travel of light rays
prototype is built around a Nikon Eclipse transmitted light microscope/wide-field fluorescence microscope and standard CCD cameras. Light field capture is obtained
Light_field_camera
under a fluorescence microscope, whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope after other
Chromogenic in situ hybridization
Chromogenic_in_situ_hybridization
combining FCCS with a confocal microscope, the technique's capabilities are highlighted, as it becomes possible to detect fluorescence molecules in femtoliter
Fluorescence cross-correlation spectroscopy
Fluorescence_cross-correlation_spectroscopy
Principle and applications of MINFLUX microscopy
mechanosensitive ion channel PIEZO1. Confocal microscopy Fluorescence Fluorescence microscope Fluorescence resonance energy transfer microscopy Laser scanning
Minflux
Computing algorithm
Simulated Fluorescence Process (SFP) is a computing algorithm used for scientific visualization of 3D data from, for example, fluorescence microscopes. By modeling
Simulated fluorescence process algorithm
Simulated_fluorescence_process_algorithm
Emission of secondary X-rays from a material excited by high-energy X-rays
X-ray fluorescence (XRF) is the emission of characteristic "secondary" (or fluorescent) X-rays from a material that has been excited by being bombarded
X-ray_fluorescence
Type of microscope
fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications. Inverted microscopes are useful for observing living
Inverted_microscope
Type of cell within the eye
false-color image of a flat-mounted rat retina viewed through a fluorescence microscope at 50x magnification. The optic nerve was injected with a fluorophore
Retinal_ganglion_cell
Lamp that produces light by an electric arc
A mercury arc lamp from a fluorescence microscope.
Arc_lamp
First phase of mitosis and meiosis
cells, centrosomes move far enough apart to be resolved using a light microscope. Microtubule activity in each centrosome is increased due to recruitment
Prophase
Device for producing light from electricity
A mercury arc lamp from a fluorescence microscope
Electric_light
Series of techniques in optical microscopy
the Abbe limit, which called for using a 4Pi microscope as a confocal laser-scanning fluorescence microscope where the light is focused from all sides to
Super-resolution_microscopy
Lab technique in biology and chemistry
have multiple lasers and fluorescence detectors. The current record for a commercial instrument is ten lasers and 30 fluorescence detectors. Increasing the
Flow_cytometry
Study of pollen and other acid-resistant microoscopic organic material
mounts on microscope slides that may be examined using a transmitted light biological microscope or ultraviolet (UV) fluorescence microscope. The abundance
Palynology
American scientific instrument manufacturer
tomography devices, NMR spectroscopy devices, fluorescence microscopes, raman spectroscopes, atomic-force microscopes, and profilometers. Bruker products are
Bruker
The first microscope to do so was the scanning tunneling microscope, which paved the way for development of the photoionization microscope and the quantum
Quantum_microscopy
Kind of single molecule imaging
Single-molecule FRET measurements are typically performed on fluorescence microscopes, either using surface-immobilized or freely-diffusing molecules
Single-molecule_FRET
Protein that exhibits bright green fluorescence when exposed to ultraviolet light
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP
Green_fluorescent_protein
Calcium imaging technique
Schnitzer, Mark J. (October 2011). "Miniaturized integration of a fluorescence microscope". Nature Methods. 8 (10): 871–878. doi:10.1038/nmeth.1694. ISSN 1548-7105
Fiber_photometry
Type of statistical analysis
Fluorescence correlation spectroscopy (FCS) is a statistical analysis, via time correlation, of stationary fluctuations of the fluorescence intensity
Fluorescence correlation spectroscopy
Fluorescence_correlation_spectroscopy
Type of carbon nanoparticle
emission of the organic dye-conjugated CQDs to green. So by using a fluorescence microscope, the organic dye-conjugated CQDs were able to visualize changes
Carbon_quantum_dot
Test to differentiate between skin fungi
identify under a fluorescence microscope. Place the slide under a microscope to read. Dermatophytes are easily recognized under the microscope by their long
KOH_test
Laser microscope used for Raman spectroscopy
The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy. The term MOLE (molecular optics laser examiner) is used to
Raman_microscope
Algorithmically generated data that have a similar distribution as sampled data
Jelena (September 2009). "Intelligent Acquisition and Learning of Fluorescence Microscope Data Models" (PDF). IEEE Transactions on Image Processing. 18 (9):
Synthetic_data
Point defect in diamonds
in a cell, and their luminescence is monitored using a standard fluorescence microscope. Stimulated emission from the NV− center has been demonstrated
Nitrogen-vacancy_center
Mineral form of calcium fluoride
larger microscope firms (Nikon, Olympus, Carl Zeiss and Leica). Their transparency to ultraviolet light enables them to be used for fluorescence microscopy
Fluorite
Branch of genetics
used to produce specific banding patterns. This method requires a fluorescence microscope and is no longer as widely used as Giemsa banding (G-banding).
Cytogenetics
Conversion of a gene's sequence into a mature gene product or products
directly quantified in live cells. This is done by imaging using a fluorescence microscope. It is very difficult to clone a GFP-fused protein into its native
Gene_expression
cytometry Fluorescence cross-correlation spectroscopy Fluorescence spectroscopy Forced Rayleigh scattering Fourier domain mode locking 4Pi microscope, a laser
List_of_laser_articles
Topics referred to by the same term
System/4 Pi, a family of avionics computers 4Pi microscope, a microscope that uses interference and fluorescence computers 4×π = 12.56637..., the solid angle
4Pi
Diffraction pattern in optics
Olivo-Marin, Jean-Christophe (2007-04-01). "Gaussian approximations of fluorescence microscope point-spread function models". Applied Optics. 46 (10): 1819–1829
Airy_disk
Antibody fragment
quality is preserved in the tissue that is imaged by fluorescence microscope and then electron microscope. This is especially useful for the neuroscience research
Single-domain_antibody
Private college in Oakland, California
Scheffler Bio-Imaging Center which contains a transilluminating fluorescence microscope with digital camera and imaging software, walk-in warm and cold
Mills_College
Branch of food chemistry
Imaging Principle Fluorescence microscope Yeast membrane protein imaging Dividing cell fluorescence HeLa cancer cells FISH fluorescence technique Red blood
Food_physical_chemistry
Fluorescence microscopy imaging method
fluorophores, the better the contrast of the fluorescence image. A single fluorophore can be visualized under a microscope (or even under the naked eye) if the
Photoactivated localization microscopy
Photoactivated_localization_microscopy
Detection of protein-protein interactions
resulting high concentration of fluorescence is easily visible as a distinct bright spot when viewed with a fluorescence microscope. In the specific case shown
Proximity_ligation_assay
Chemical compound
can be readily visualized in both living and fixed cells using a fluorescence microscope. Lucifer yellow was invented by Walter W. Stewart at the National
Lucifer_yellow
Type of microscope that uses X-rays
An X-ray microscope uses electromagnetic radiation in the X-ray band to produce magnified images of objects. Since X-rays penetrate most objects, there
X-ray_microscope
Branch of petrology focusing on detailed descriptions of rocks
as well as whole rock chemical analysis by atomic absorption, X-ray fluorescence, and laser-induced breakdown spectroscopy are used in a modern petrographic
Petrography
Scientific method
viewed using a fluorescence microscope and captured by a Scientific CMOS (sCMOS) camera or CCD camera. Confocal and two-photon microscopes provide optical
Calcium_imaging
Chemical compound
"Medical Definition of ANILINE BLUE". www.merriam-webster.com. "Fluorescence Microscope Images". Archived from the original on 2008-05-26. Retrieved 2012-01-14
Aniline_Blue_WS
Light re-emitted by chlorophyll molecules during return from excited to non-excited states
Chlorophyll fluorescence is light re-emitted by chlorophyll molecules during return from excited to non-excited states. It is used as an indicator of photosynthetic
Chlorophyll_fluorescence
Optical system with resolution performance at the instrument's theoretical limit
In optics, any optical instrument or system – a microscope, telescope, or camera – has a principal limit to its resolution due to the physics of diffraction
Diffraction-limited_system
Light energy absorbance and re-emission
Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter threshold wavelength, a phosphorescent
Phosphorescence
resolution, but this is not acceptable in many applications. In a fluorescence microscope, resolution in the z-direction is bad as it is. More advanced image
Image restoration by artificial intelligence
Image_restoration_by_artificial_intelligence
Imaging molecules within living patients
techniques including live-cell microscopy, total internal reflection fluorescence microscope (TIRF)-microscopy, stimulated emission depletion (STED)-nanoscopy
Molecular_imaging
Study of the microscopic anatomy of cells and tissues of plants and animals
to gross anatomy, which looks at larger structures visible without a microscope. Historically, microscopic anatomy was divided into organology, the study
Histology
Optical microscopy technique
reveals many cellular structures that are invisible with a bright-field microscope, as exemplified in the figure. These structures were made visible to earlier
Phase-contrast_microscopy
Biochemical method
subset of cells will now fluoresce green and can be followed with a fluorescence microscope. Next, to figure out what these cells might do, the experimenter
GAL4/UAS_system
Science Museum in Leiden, Netherlands
a cryomicrotome) and ultramicrotomes, a phase contrast microscope, a fluorescence microscope (plus several epi-illuminators, to exploit the difference
Museum_Boerhaave
Ability of any image-forming device to distinguish small details of an object
of any image-forming device such as an optical or radio telescope, a microscope, a camera, or an eye, to distinguish small details of an object, thereby
Angular_resolution
Biochemical identification method
are bound to a probe which is then hybridized to chromosomes. A fluorescence microscope can detect the dyes present and send it to a computer that can
Fluorescent_tag
Protein-coding gene in the species Homo sapiens
cells and necrotic cells. Detection occurs by flow cytometry or a fluorescence microscope. Annexin A5 has been shown to interact with Kinase insert domain
Annexin_A5
Pericyclic chemical reaction
is fluorescently labeled, and can therefore be imaged using a fluorescence microscope. To avoid toxicity of copper(I), Bertozzi et al. developed the
1,3-Dipolar_cycloaddition
Allotropes of carbon with a cylindrical nanostructure
emitters can be used. An alternative is to measure fluorescence lifetimes instead of fluorescence intensities. Overall, SWCNTs therefore have great potential
Carbon_nanotube
Chemical compound
applied to study membrane fluidity of live cells with a 2-Photon fluorescence microscope in 1994 and it was found that the plasma membrane of cells is more
Laurdan
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
Girl/Female
Biblical
Fighting, chiding, multiplying.
Boy/Male
Polish
Good.
Surname or Lastname
German
German : nickname for a calm individual, variant of Still 1.English : topographic name for someone who lived by a fish trap in a river (see Still 2).German : habitational name from Still in Alsace.
Boy/Male
Gujarati, Hindu, Indian, Kannada
Feeling
Male
English
Lover of Hounds
Girl/Female
Hindu, Indian, Malayalam
Butterfly; Goddess Lakshmi
Boy/Male
Hindu
To search
Boy/Male
Indian
God of Fame
Boy/Male
Bengali, Indian
King
Boy/Male
Indian
One who prays times and fasts, Forever, Immortal
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
FLUORESCENCE MICROSCOPE
a.
Producing, or relating to, epipolism or fluorescence.
n.
A hydrocarbon (C13H10) obtained in white fluorescent crystals, in the distillation products of the needles of the California "big tree" (Sequoia gigantea).
n.
A beautiful fluorescent crystalline substance, intermediate in composition between thionol and thionine.
n.
A small or secondary spike; especially, one of the ultimate parts of the in florescence of grasses. See Illust. of Quaking grass.
a.
Changed to the epipolic condition, or that in which the phenomenon of fluorescence is presented; produced by fluorescence; as, epipolized light.
n.
A blue, fluorescent, oily base (regarded as a derivative of pyridine), obtained from conine.
n.
A hydrocarbon (C/H/) extracted from the pitchy residue of coal tar and petroleum as a bluish fluorescent crystalline substance.
n.
A compound which exhibits, or from which may be prepared, a variety of colors, as certain solutions derived from vegetables, which display colors by fluorescence.
n.
That property which some transparent bodies have of producing at their surface, or within their substance, light different in color from the mass of the material, as when green crystals of fluor spar afford blue reflections. It is due not to the difference in the color of a distinct surface layer, but to the power which the substance has of modifying the light incident upon it. The light emitted by fluorescent substances is in general of lower refrangibility than the incident light.
n.
A bursting into flower; a blossoming.
n.
A glucoside obtained from the Aesculus hippocastanum, or horse-chestnut, and characterized by its fine blue fluorescent solutions.
n.
The act, process, or time of flowering; florescence.
n.
An instrument for observing or exhibiting fluorescence.
n.
Esculin; -- so called in allusion to its fluorescent solutions.
a.
Having the property of fluorescence.
n.
A complex hydrocarbon, C14H10, found in coal tar, and obtained as a white crystalline substance with a bluish fluorescence.
n.
A colorless, crystalline hydrocarbon, C13H10 having a beautiful violet fluorescence; whence its name. It occurs in the higher boiling products of coal tar, and is obtained artificially.
n.
The coloring principle of turmeric, or curcuma root, extracted as an orange yellow crystalline substance, C14H14O4, with a green fluorescence.
n.
See Fluorescence.
n.
A hydrocarbon obtained from coal-tar residues, and remarkable for its intense yellowish green fluorescence.