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Imaging technique based on fluorescence
Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission
Fluorescence-lifetime imaging microscopy
Fluorescence-lifetime_imaging_microscopy
Type of non-invasive imaging technique
organism. Fluorescence images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy. Fluorescence itself, is
Fluorescence_imaging
Fluorescence imaging technique
Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue
Two-photon excitation microscopy
Two-photon_excitation_microscopy
Fluorescence microscopy technique
Light-sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate-to-high optical resolution, but good optical sectioning
Light sheet fluorescence microscopy
Light_sheet_fluorescence_microscopy
Technique in fluorescence microscopy
emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation
STED_microscopy
Series of techniques in optical microscopy
Super-resolution microscopy is a series of super-resolution imaging techniques in optical microscopy that allow such images to have resolutions higher
Super-resolution_microscopy
Method for displaying sine wave vectors
wave forms is realized by their vectorial summation. In Fluorescence lifetime and spectral imaging, phasor can be used to visualize the spectra and decay
Phasor approach to fluorescence lifetime and spectral imaging
Phasor_approach_to_fluorescence_lifetime_and_spectral_imaging
Emission of light by a substance that has absorbed light
bound can be seen, and even quantified, by the fluorescence. FLIM (Fluorescence Lifetime Imaging Microscopy) can be used to detect certain bio-molecular
Fluorescence
Simultaneous measurement of spectra and pictures
sensing of green fluorescent proteins in living cells using fluorescence lifetime imaging microscopy. Biophys J. (2008) 94(8):L67-9. Van der Krogt GNM, et al
Chemical_imaging
Experimental technique in cell biology
illumination which causes their fluorescence lifetime to quickly elapse (limited to roughly 105 photons before extinction). Now the image in the microscope is that
Fluorescence recovery after photobleaching
Fluorescence_recovery_after_photobleaching
Photochemical energy transfer mechanism
acceptor. Lifetime measurements of the FRET-donor are used in fluorescence-lifetime imaging microscopy (FLIM). smFRET is a group of methods using various microscopic
Förster resonance energy transfer
Förster_resonance_energy_transfer
Non-linear optical imaging modality
is commonly used for fluorescence-lifetime imaging microscopy (FLIM) data analysis and has been adapted for pump–probe imaging data analysis. Signals
Pump–probe_microscopy
Type of electromagnetic spectroscopy
interactions. Initiated in the early 2000s, TRFPs enhance fluorescence lifetime imaging microscopy (FLIM), offering superior contrast over intensity-based
Fluorescence_spectroscopy
Topics referred to by the same term
probability "Phosphorescence Lifetime Imaging Microscopy" (PLIM) - An imaging technique similar to Fluorescence-lifetime imaging microscopy but based on phosphorescence
Plim
Computational imaging technique
counting (TCSPC), the use of single-pixel imaging for compressive fluorescence lifetime imaging microscopy (FLIM) has also been explored. Since the late
Single-pixel_imaging
Topics referred to by the same term
or FLIM may refer to: Fermanagh Light Infantry Militia Fluorescence-lifetime imaging microscopy Jimmy Johnson (bassist) (born 1956), American bass guitarist
Flim
Counting photons using a single-photon detector
Medical imaging Light detection and ranging DNA sequencing Astrophysics Materials science In radiology, one of the major disadvantages of X-ray imaging modalities
Photon_counting
Loss of colour by a pigment when illuminated
needed] This use of the term "lifetime" is not to be confused with the "lifetime" measured by fluorescence lifetime imaging. Ozone depletion Demchenko,
Photobleaching
Type of electron microscope
"Scanning electron microscopy (SEM) – imaging". Microscopy Australia. 30 November 2025. "SEM Basics". MyScope training, Microscopy Australia. 1 December
Scanning_electron_microscope
Scientific method
Calcium imaging is a microscopy technique to optically measure the calcium (Ca2+) status of an isolated cell, tissue or medium. Calcium imaging takes advantage
Calcium_imaging
Natural emission of light by biological structures
super resolution microscopy SPDM revealed autofluorescent cellular objects which are not detectable under conventional fluorescence imaging conditions. Substances
Autofluorescence
Indian American biophysicist and academic
imaging systems, including confocal, multi-photon and fluorescence-lifetime imaging microscopy base devices. Periasamy was born in India. He trained at
Ammasi_Periasamy
Heat-activated light emission
shown success in the field of bioimaging as its long lifetime allows time-resolved fluorescence imaging in living cells. These tailored organic compounds
Thermally activated delayed fluorescence
Thermally_activated_delayed_fluorescence
technique is applied to a microscopy imaging. Whereas the measurement protocol equals fluorescence lifetime imaging microscopy (FLIM), the read-out parameter
Fluorescence intensity decay shape microscopy
Fluorescence_intensity_decay_shape_microscopy
Synthetic organic compound used as dye and fluorescent tracer
Joe T.; Gillette, Amani A.; Skala, Melissa C. (2020). "Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis
Fluorescein
Medical intervention
(i.e., fluorescence-lifetime imaging microscopy or FLIM), or by analyzing the spectral shape of emitted fluorescence (fluorescence spectroscopy). Near-infrared
Intravascular_fluorescence
Light emission from substances after they absorb photons
microscopy, to map the intensity (confocal microscopy) or the lifetime (fluorescence-lifetime imaging microscopy) of the photoluminescence across a sample
Photoluminescence
Scientific investigative technique
quantitative, 3D view of the sample. Fluorophore Fluorescent microscopy Fluorescence imaging Fluorescent glucose biosensors Fluoroscopy Gustavsson, Thomas;
Fluorescence in the life sciences
Fluorescence_in_the_life_sciences
Research Institute at the University of Oxford
Fluorescence Lifetime Correlation Spectroscopy (FLCS), Fluorescence Lifetime Imaging Microscopy (FLIM), Total Internal Reflection Fluorescence Microscopy (TIRF)
Wellcome Centre for Human Genetics
Wellcome_Centre_for_Human_Genetics
American biochemist
spectroscopy, microscopy and imaging . Amongst others, Lakowicz coined the term fluorescence lifetime imaging microscopy (FLIM) and in the mid-1990s his
Joseph_R._Lakowicz
birefringence Fluorescence anisotropy FLIM – Fluorescence lifetime imaging Fluorescence microscopy FOSPM – Feature-oriented scanning probe microscopy FRET –
List of materials analysis methods
List_of_materials_analysis_methods
Category of polyphenol compound
Claisen-like reactions to occur, generating aromatic rings. Fluorescence-lifetime imaging microscopy can be used to detect flavanols in plant cells. Schematic
Flavan-3-ol
Diffusion-weighted imaging, a type of MRI that uses water diffusion Fluorescence lifetime imaging, using the decay rate of a fluorescent sample Gallium imaging, a nuclear
Biological_imaging
Scientific discipline
that results in a change in fluorescence. FRET has also been used in tandem with Fluorescence Lifetime Imaging Microscopy (FLIM) or fluorescently conjugated
Chemical_biology
Hyperspectral imaging Full Spectral Imaging List of Earth observation satellites Chemical Imaging Infrared Microscopy Phasor approach to fluorescence lifetime and
Imaging_spectrometer
Protein that exhibits bright green fluorescence when exposed to ultraviolet light
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP
Green_fluorescent_protein
Microscope imaging technique
(2016). "SHG Microscopy and Its Comparison with THG, CARS, and Multiphoton Excited Fluorescence Imaging". Second Harmonic Generation Imaging, 2nd edition
Second-harmonic imaging microscopy
Second-harmonic_imaging_microscopy
Agents that emit light after excitation by light
active in fluorescence emission. For quantum dots, prolonged single-molecule microscopy showed that 20-90% of all particles never emit fluorescence. On the
Fluorophore
applying to standard microscopy using transmission or reflection of natural light (as opposed to structured illumination or fluorescence) according to the
Vertico spatially modulated illumination
Vertico_spatially_modulated_illumination
Technology used to validate protein interactions
using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional
Bimolecular fluorescence complementation
Bimolecular_fluorescence_complementation
Hyperspectral Imaging for Food Quality Analysis and Control. Elsevier. ISBN 9780080886282. Modulated CMOS camera for fluorescence lifetime microscopy, Vladimir
PCO_Imaging
Developer and manufacturer of high performance light measuring solutions
such as plasma studies or fluorescence lifetime imaging Andor’s microscopy systems are used in life sciences (neuron imaging, stem cell research, developmental
Andor_Technology
Spectroscopy with lasers with very short pulses
Electron Diffraction imaging, Kerr Gated Microscopy, imaging with ultrafast electron pulses and terahertz imaging. This is particularly true in the biomedical
Ultrafast_laser_spectroscopy
Super-resolution dipole orientation mapping (SDOM) is a form of fluorescence polarization microscopy (FPM) that achieved super resolution through polarization
Super-resolution dipole orientation mapping
Super-resolution_dipole_orientation_mapping
Type of energy transfer
Time-resolved fluorescence energy transfer (TR-FRET) is the practical combination of time-resolved fluorometry (TRF) with Förster resonance energy transfer
Time-resolved fluorescence energy transfer
Time-resolved_fluorescence_energy_transfer
Yellow food coloring
utility of tartrazine to enable deep-tissue Raman sensing and fluorescence lifetime imaging. Sunset yellow FCF, also known as Yellow 6 Food Standards Australia
Tartrazine
Italian-American biophysicist
deformation. Phasor approach is a model-free analysis method used in fluorescence lifetime imaging to map cellular metabolism. This technique has been widely adopted
Enrico_Gratton
microscopy, two photon microscopy, confocal microscopy, total internal reflection fluorescence microscopy, fluorescence lifetime imaging microscopy,
Centre_for_Genomic_Regulation
Chemical compound
organic compound which is used as a fluorescent dye when applied to fluorescence microscopy. It is used to investigate membrane qualities of the phospholipid
Laurdan
Non-invasive analytical tool
the lifetimes of the excited states are long, which allows the use of time resolved spectroscopy, a definitive asset for bioassays and microscopy. The
Lanthanide_probes
biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated
Live_single-cell_imaging
the DO-SRS microscopy platform, offering a noninvasive approach to imaging metabolic dynamics in living animals. DO-SRS metabolic imaging has advanced
Lingyan_Shi
resolution. More recently, single-molecule fluorescence is the subject of intense interest for biological imaging, through the labeling of biomolecules such
Single-molecule_experiment
utility of tartrazine to enable deep-tissue Raman sensing and fluorescence lifetime imaging. In addition to tartrazine, several other absorbing dye molecules
Tissue_clearing
Fluorescent microthermography (FMT) is a microscopy technique for infrared imaging of temperature distribution on a small scale. It can achieve a spatial
Fluorescent_microthermography
Biological membrane structure
traditional microscope because it is too thin, so researchers often use fluorescence microscopy. A sample is excited with one wavelength of light and observed
Lipid_bilayer
Simultaneous absorption of two photons of differing energies by a molecule
two-photon microscopy, allowing for fluorescent emission deeper in a sample. At every depth location in a sample, ND-TPA provides brighter fluorescence than
Non-degenerate two-photon absorption
Non-degenerate_two-photon_absorption
of Ion Concentrations". Fluorescence Microscopy of Living Cells in Culture Part B. Quantitative Fluorescence Microscopy—Imaging and Spectroscopy. Methods
Molecular_sensor
Allotropes of carbon with a cylindrical nanostructure
September 2015). "Hyperspectral Microscopy of Near-Infrared Fluorescence Enables 17-Chirality Carbon Nanotube Imaging". Scientific Reports. 5 (1) 14167
Carbon_nanotube
American biomedical engineer
graduate student at Duke University, where she developed fluorescence-lifetime imaging microscopy (FLIM) for the diagnosis of neoplasia. After graduating
Melissa_Skala
Art and practice of creating images by recording light
organ or cavity of the body. Fluorescence lifetime imaging – using the decay rate of a fluorescent sample. Fluorescence image-guided surgery – used to detect
Outline_of_photography
Concept in optics
tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy". Journal of Physics D: Applied Physics. 37
Supercontinuum
multi-modal optical imaging. This involves fluorescence-lifetime imaging microscopy for initial guidance and reflectance confocal microscopy for the detection
Kristen_Maitland
Form of electromagnetic radiation
10 nm – Medical Imaging Systems". 3 August 2018. in: Berger, Martin; Yang, Qiao; Maier, Andreas (2018). "X-ray Imaging". Medical Imaging Systems: An Introductory
X-ray
Kind of single molecule imaging
order to generate sufficient fluorescence signals to enable single-molecule detection. Wide-field multiphoton microscopy is typically combined with total
Single-molecule_FRET
Nano-scale semiconductor particles
integrated at a scanning microscopy tip was used to demonstrate fluorescence near-field scanning optical microscopy (NSOM) imaging. Quantum dot photodetectors
Quantum_dot
German chemist and academic (born 1962)
Thomas; Seeger, Stefan (11 May 2004). "Deep-UV Laser-Based Fluorescence Lifetime Imaging Microscopy of Single Molecules". The Journal of Physical Chemistry
Stefan_Seeger
Chemical compound
used to improve the lifetime of dyes. This makes DABCO useful in dye lasers and in mounting samples for fluorescence microscopy (when used with glycerol
DABCO
Small container used in laboratories
measure the absorbance, transmittance, fluorescence intensity, fluorescence polarization, or fluorescence lifetime of the sample. This measurement is done
Cuvette
Nigerien-American biophysicist
Paris, Cissé developed the single-cell microscopy technique time-correlated photoactivated localization microscopy (tcPALM), allowing for time resolved
Ibrahim_Cissé_(academic)
spectroscopy, magnetic resonance imaging (MRI), dual-energy computed tomography, fluorescence-lifetime imaging microscopy, multispectral optoacoustic tomography
Immunophysics
Canadian manufacturer of scientific instruments
(18 August 2015). "Hyperspectral Microscopy of Near-Infrared Fluorescence Enables 17-Chirality Carbon Nanotube Imaging". Scientific Reports. 5 14167. Bibcode:2015NatSR
Photon_etc.
Large-scale synchrotron facility in Australia
use EPICS as the basis for their control. Imaging and Medical Beamline (IMBL) X-ray Fluorescence Microscopy (XFM) beamline Macromolecular and Micro crystallography
Australian_Synchrotron
American engineer
laboratory has developed time-resolved fluorescence spectroscopy (TRFS) and fluorescence lifetime imaging microscopy (FLIM) systems for in vivo tissue interrogation
Laura_Marcu
Practical physics application
beneficial as excitation sources for fluorescence imaging. Fluorescence near-field scanning optical microscopy (NSOM) utilizing an integrated QD-LED
Light-emitting_diode_physics
Photoluminescent particles
emission-excitation differences of 10–100 kBT and an observable fluorescence lifetime after the excitation source has been switched off. Photon upconversion
Upconverting_nanoparticles
Excited state dynamic
quintet state. Fluorescence microscopy relies upon fluorescent compounds, or fluorophores, in order to image biological systems. Since fluorescence and phosphorescence
Intersystem_crossing
Spectroscopic technique
10–50 mm in diameter. Spectroscopic image resolution levels of 200 nm or below has been achieved on latest imaging XPS instruments using synchrotron radiation
X-ray photoelectron spectroscopy
X-ray_photoelectron_spectroscopy
Family of fluorescent dyes
Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to
Alexa_Fluor
Synchrotron light source facility in Saskatoon, Canada
Royal Navy sailors buried in Antigua in the late 1700s. They used X-ray fluorescence to look for trace elements such as lead and strontium in bones from a
Canadian_Light_Source
Academic award
for strongly correlated oxides 2013 Paul French, for fluorescence-lifetime imaging microscopy 2011 Donald D Arnone, for terahertz radiation research
James_Joule_Medal_and_Prize
Type of mathematical relation
(2025-12-15). "Evaluating tartrazine as an optical clearing agent for fluorescence lifetime imaging". Optics Letters. 50 (24): 7588–7591. Bibcode:2025OptL...50
Kramers–Kronig_relations
with the human eye, the spectral differences are too small for fluorescence microscopy filters. Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl
FMN-binding fluorescent protein
FMN-binding_fluorescent_protein
Insect protein
409 nm emissions, the rec1-Resilin begins to show blue fluorescence. An example of the blue fluorescence exhibited by the dityrosine residues in resilin is
Resilin
Extremely small diamonds used for their thermal, mechanical and optoelectronic properties
a stable reference for the quality control purposes in fluorescence and multiharmonic imaging systems. 2012 Ig Nobel Peace Prize: The SKN Company, for
Nanodiamond
Coenzyme
Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML (1992). "Fluorescence lifetime imaging of free and protein-bound NADH". Proc. Natl. Acad. Sci. U.S
Nicotinamide adenine dinucleotide
Nicotinamide_adenine_dinucleotide
Read-out via fluorescence lifetime imaging (FLIM) of donor fluorescence is also possible, as the FRET process speeds up the fluorescence decay. can be
Optogenetic methods to record cellular activity
Optogenetic_methods_to_record_cellular_activity
Synchrotron light source in Kraków, Poland
nano-infrared spectroscopy coupled with atomic force microscopy and scanning near-field microscopy (AFM-SNOM-FTIR imaging). Application: in biomedicine, nanotechnology
Solaris_(synchrotron)
American physical chemist
sensing. His team has used combinations of optical and scanning probe microscopy to examine the properties of nanomaterials, particularly thin film semiconductors
David_Ginger
fluorescence interference contrast microscopy (FLIC) and reflection interference contrast microscopy (RICM) or interferometric scattering microscopy (iSCAT)
Model_lipid_bilayer
Combination in the membranes of cells
Resonance (NMR) although fluorescence microscopy remains the dominant technique. In the future it is hoped that super-resolution microscopy such as Stimulated
Lipid_raft
Point defect in diamonds
it was demonstrated that the fluorescence of single NV− centers can be detected by room-temperature fluorescence microscopy and that the defect shows perfect
Nitrogen-vacancy_center
Biological structure
spread of pathogens or toxins such as HIV and prions. TNTs have observed lifetimes ranging from a few minutes up to several hours, and several proteins have
Tunneling_nanotube
Single-molecule imaging technique
This arrangement enables high-resolution imaging of DNA using total internal reflection fluorescence microscopy (TIRFM). This is the earliest and simplest
DNA_curtain
Wavelength-selective optical filter
lights can be identified by the IEC 60598 No Cool Beam symbol. In fluorescence microscopy, dichroic filters are used as beam splitters to direct illumination
Interference_filter
Single-celled algae with silica cell walls
investigations have involved, and been propelled by, diverse approaches including, microscopy, chemistry, biochemistry, material characterisation, molecular biology
Diatom
Electric current produced in living cells
Li, Gang; Porterfield, David Marshall (2009). "Frequency-domain fluorescence lifetime optrode system design and instrumentation without a concurrent reference
Developmental_bioelectricity
Spectroscopy 13.1a Time-resolved Confocal Fluorescence Lifetime Imaging and Spectroscopy 13.1b Time-resolved Fluorescence Spectroscopy 13.2 Synchrotron Light
Synchrotron_Radiation_Source
Protozoan parasitic disease
nucleus being one-third of its size. It can be identified by electron microscopy or by Giemsa staining tissue where the cytoplasm shows blue, the nucleus
Toxoplasmosis
American physicist
20.000426. ISSN 0146-9592. "Fluorescence lifetime imaging in turbid media", Fluorescence Lifetime Spectroscopy and Imaging, CRC Press, 2014-07-17, pp. 300–341
Arjun_Yodh
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