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Natural emission of light by biological structures
Autofluorescence is the natural fluorescence of biological structures (autofluorophores) such as mitochondria and lysosomes, in contrast to fluorescence
Autofluorescence
Genus of cockroaches
detailed research has conclusively demonstrated otherwise, although autofluorescence has been documented, such that the spots will glow when exposed to
Lucihormetica
Foodborne protozoan infection
modified acid-fast staining for variable staining (from pale to red), and autofluorescence with ultraviolet lights are used. Obtaining these oocysts is usually
Cyclosporiasis
Insect-parasitic micro-organism
host multiple species. A remarkable feature of G. garnhami is its autofluorescence.[citation needed] Gregarina garnhami was considered by Lipa et al.
Gregarina_garnhami
Alveolar macrophages whose characteristics have been affected by smoking
tar from cigarettes accumulates in alveolar macrophages and causes autofluorescence. The intensity of fluorescence, however, is independent of cigarette
Smoker's_macrophages
Family of trombidiform mites
in yards or gardens or in compost bins. In 2018, methods based on autofluorescence microscopy were developed to enable identification of trombiculid mites
Trombiculidae
Industrial and food chemical
possesses superior optical properties, allowing for minimal background and autofluorescence during fluorescent imaging. In biomedical (or biological) microelectromechanical
Polydimethylsiloxane
Species of bacterium
fulgida's 288-330 nm autofluorescence increases with increasing OD and has the highest intensity of all three peaks, the autofluorescence intensity at 288-478 nm
Brocadia_fulgida
Optical microscope that uses fluorescence and phosphorescence
protein. Alternatively the intrinsic fluorescence of a sample (i.e., autofluorescence) can be used. In the life sciences fluorescence microscopy is a powerful
Fluorescence_microscope
Protein filament that grows from follicles found in the dermis, or skin
across a wide range of wavelengths. The image above depicts melanin autofluorescence at 365-400 nm excitation from a strand of dark brown human hair.
Hair
Genus of trees
(Turkish hazel) Hazelnuts Closeup of a male hazelnut flower using autofluorescence microscopy "Corylus L." Plants of the World Online. Royal Botanic Gardens
Hazel
Chemical compound
the condition, such as mottled areas in the RPE, patches of abnormal autofluorescence, and deposits beneath the retina. These features can help distinguish
Pentosan_polysulfate
Loss of colour by a pigment when illuminated
(primarily antibody-linked) fluorescent molecules, in an attempt to quench autofluorescence. This can help improve the signal-to-noise ratio. Photobleaching may
Photobleaching
Planned 2020s private Venus astrobiology probe
acceptable temperatures. The probe contains one scientific instrument; the Autofluorescence Nephelometer, which will project a 440 nm diode laser into the Venusian
Venus_Life_Finder
Giant cockroach species with fluorescent carapace
features one small and two large spots that glow when exposed to light (autofluorescence), perhaps to mimic the appearance of the toxic click beetle (Pyrophorus)
Lucihormetica_luckae
Species of cockroach
mate choice by the female, or may provide warning signals, though autofluorescence has been documented, such that the spots will glow when exposed to
Lucihormetica_verrucosa
Technology used to validate protein interactions
produced by expression of intact fluorescent proteins, the extremely low autofluorescence in the visible range extremely most cells often makes the BiFC signal
Bimolecular fluorescence complementation
Bimolecular_fluorescence_complementation
Emission of light by a substance that has absorbed light
cellular components are naturally fluorescent (called intrinsic or autofluorescence). In fact, a protein or other component can be "labelled" with an extrinsic
Fluorescence
Medical intervention
technique that uses near-infrared fluorescence to detect artery wall autofluorescence (NIRAF) or fluorescence generated by molecular agents injected intravenously
Intravascular_fluorescence
Blood glucose measurement device
W284 in the N-terminal domain (which can bind calcium), affect the autofluorescence spectra upon glucose binding. Some studies with Ggbp and solvatochromic
Fluorescent_glucose_biosensor
Advanced form of age-related macular degeneration
autofluorescence and optical coherence tomography angiography are imaging modalities that can be used in the diagnosis. While fundus autofluorescence
Geographic_atrophy
Spectroscopic technique
this spectral range, the risk of damaging the specimen as well as autofluorescence emission are reduced, and high penetration depths into tissues can
Raman_spectroscopy
Single-celled alga with a silica cell wall
fluorescence] - stains DNA, identifies nuclei (d) Red: [chlorophyll autofluorescence] - resolves chloroplasts The animation starts by overlaying all available
Diatom
Technique used for light microscopy
immunofluorescence techniques include autofluorescence, spectral overlap and non-specific staining. Autofluorescence includes the natural fluorescence emitted
Immunofluorescence
Species of flowering plant
bright field, differential interference contrast, phase contrast, autofluorescence) List of Scilla species "Scilla siberica Andrews". Plants of the World
Scilla_siberica
American nanotechnologist
these nanoparticles, important because time-gating suppresses tissue autofluorescence that often compromises the fidelity of fluorescence images of biological
Michael_J._Sailor
Organism composed of cells of different genotypes
plant development stage and GFP may be influenced by the green tissue autofluorescence. Quantitative PCR could be an alternative method for chimera detection
Chimera_(genetics)
American ophthalmologist
surgery, macular diseases, and ocular imaging technologies such as autofluorescence Imaging and optical coherence tomography (OCT). He has designed surgical
Richard_F._Spaide
Light re-emitted by chlorophyll molecules during return from excited to non-excited states
guard cells and pavement cells (above). Same region showing Chlorophyll A autofluorescence with 440 nm laser excitation and far red emission (below).
Chlorophyll_fluorescence
Blue-light toxicity
examinations and observed markedly less "progression of abnormal fundus autofluorescence"; however the authors failed to discuss the fact that the excitation
Biological effects of high-energy visible light
Biological_effects_of_high-energy_visible_light
by Force-A. The sensor is a result of 15 years of research on plant autofluorescence conducted by the CNRS (National Center for Scientific Research) and
Multiplex_(sensor)
Law of photochemistry
Wilkinson, A. Blackwell Scientific Publications, Oxford, 1997. "Unusual autofluorescence characteristic of cultured red-rain cells". Louis, J. and Kumar, A
Kasha's_rule
Viewing of objects which are too small to be seen with the naked eye
is known as fluorescence. Often specimens show their characteristic autofluorescence image, based on their chemical makeup. This method is of critical importance
Microscopy
Medical condition
shows characteristic bilateral scalloped, peripheral lesions, Fundus autofluorescence imaging demonstrating patchy hypo-autofluorescent lesions peripherally
Ornithine aminotransferase deficiency
Ornithine_aminotransferase_deficiency
Scientific method
reporters may also hold potential because of their ability to abrogate autofluorescence, photobleaching [no excitation wavelength is needed], biological degradation
Calcium_imaging
Genus of single-celled organisms
acantharian hosting Phaeocystis symbionts. Red fluorescence is chlorophyll autofluorescence and allows observation of the altered morphology of Phaeocystis chloroplasts
Phaeocystis
Scientific investigative technique
are naturally fluorescent, which is called intrinsic fluorescence or autofluorescence (such as NADH, tryptophan or endogenous chlorophyll, phycoerythrin
Fluorescence in the life sciences
Fluorescence_in_the_life_sciences
Chemical compound
before the CFSE fluorescence is too low to be distinguished above the autofluorescence background. Thus CFSE represents an extremely valuable fluorescent
Carboxyfluorescein succinimidyl ester
Carboxyfluorescein_succinimidyl_ester
Class of chemical substance
wavelengths must occur in regions with minimal overlap from sample autofluorescence, other absorbing species, and maximum tissue penetration. Regarding
Metal–organic_framework
Imaging technique
approaches—including structural OCT, OCT angiography (OCTA), and fundus autofluorescence—may be used in longitudinal studies to evaluate the temporal persistence
Optical_coherence_tomography
Lab technique in biology and chemistry
subsequently unmixed by using reference spectra of all used dyes and the autofluorescence spectrum. This may allow for a wider panel design and the application
Flow_cytometry
Cancer of the lining of the lips, mouth, or upper throat
Studies have used optical coherence tomography, photographic imaging, autofluorescence spectroscopy, and thermal imaging, showing high variation in approach
Oral_cancer
Episodes of rain containing vast amounts of red algal spores in India
(help) Godfrey Louis & A. Santhosh Kumar (28 August 2008). "Unusual autofluorescence of cultured red-rain cells". SPIE Digital Library. 7097. SPIE Symposium
Red_rain_in_Kerala
Disease of the retina affecting central vision
angiography (which may identify an abnormal capillary pattern), fundus autofluorescence, and OCT. These can help to identify the abnormal vessels, pigment
Macular_telangiectasia
Optical process
nanoparticles can be excited with near-infrared light, they can reduce autofluorescence of biological samples and thus improve the contrast of the image. Lanthanide-doped
Photon_upconversion
side of the system is not covered by the material which can reduce autofluorescence during imaging. Further, open systems minimize and sometimes eliminate
Open_microfluidics
Microscope observing a thin depth of a cell
light exposure and using immersion oils to minimize light scattering. Autofluorescence can occur in certain cell structures where the natural compound in
Total internal reflection fluorescence microscope
Total_internal_reflection_fluorescence_microscope
Class of chemical compounds
histological dye specific to polyphenols used in microscopy analyses. The autofluorescence of polyphenols can also be used, especially for localisation of lignin
Polyphenol
Allotropes of carbon with a cylindrical nanostructure
where optical sensor applications benefit from reduced scattering and autofluorescence of biological samples and consequently a high signal-to-noise ratio
Carbon_nanotube
Genetic testing technique
The use of ethanol washes are typically used at this stage to reduce autofluorescence in tissues or cells. At the end of the assay the tissue samples are
Fluorescence in situ hybridization
Fluorescence_in_situ_hybridization
Technique for examining the circulation of the retina and choroid of the eye
seconds after injection). The early images allow for the recognition of autofluorescence of the retinal tissues. Photos are taken approximately once every second
Fluorescein_angiography
Medical condition
syndrome can be made through ocular investigations (such as fundus autofluorescence, electroretinogram and opticam coherence tomography) alongside sequencing
Goldmann–Favre_syndrome
Optical waveguide
October 2019). "Deep Ultraviolet Plasmonic Enhancement of Single Protein Autofluorescence in Zero-Mode Waveguides". Nano Letters. 19 (10): 7434–7442. arXiv:1909
Zero-mode_waveguide
Topics referred to by the same term
figurines, an idiosyncratic type of sculpture in Cycladic art Fundus autofluorescence, an imaging technique for studying the retina This disambiguation page
FAF
Medical condition
The retinal pigment epithelium (RPE) can be captured as well, using autofluorescence, because the light waves can detect lipofuscin. A B-scan ultrasound
Choroidal_nevus
Visual maladies
Lukas Reznicek, Carmen Stumpf (15 August 2014). "Role of wide-field autofluorescence imaging and scanning laser ophthalmoscopy in differentiation of choroidal
Visual_pathway_lesions
Light emission in lifeforms with external source
ISSN 0166-8595. PMID 24458602. Roshchina, Victoria V. (2012-09-10). "Vital Autofluorescence: Application to the Study of Plant Living Cells". International Journal
Biofluorescence
Measure the amount of a target entity
quenching reagent in a fluorescence detection system that prevents "autofluorescence" of background objects. [citation needed] Depending on whether an assay
Assay
Hybrid of engineering, cybernetics, and robotics
and 20 h after bombardment. This is a useful method if chlorophyll autofluorescence is a major impediment to observation since achlorophyllous cells such
Biorobotics
American bioengineer and academic
Lotan, Reuben; Follen, Michele; Richards-Kortum, Rebecca (June 2001). "Autofluorescence Microscopy of Fresh Cervical-Tissue Sections Reveals Alterations in
Rebekah_Drezek
Genetic mitochondrial disease
the posterior fundus which correspond to granular patterns on fundus autofluorescence imaging. Associated changes on optical coherence tomography (OCT) include
Kearns–Sayre_syndrome
Medical condition
presentation, optical coherence tomography (loss of IS/OS junctions), fundus autofluorescence (focal hyper or hypoautofluorescence), and multifocal electroretinography
Chloroquine_retinopathy
Calcium imaging technique
calcium-independent fluorescence baseline. Animal motion and tissue autofluorescence are reflected in this calcium-independent signal and can be subtracted
Fiber_photometry
intensity value. This way, fluorescence signal, which originates from autofluorescence background and therefore exhibits increased error-values, is divided
Fluorescence intensity decay shape microscopy
Fluorescence_intensity_decay_shape_microscopy
Genetic form of macular degeneration
may include Scanning laser ophthalmoscopy which highlights areas of autofluorescence which are associated with retinal pathology. Spectral-domain optical
Stargardt_disease
Italian engineer and inventor
Coronary Atherosclerosis With Dual-Modality OCT and Near-Infrared Autofluorescence Imaging". J Am Coll Cardiol Img. 9 (11): 1304–1314. doi:10.1016/j.jcmg
Giovanni_J._Ughi
Medical condition
ophthalmologists to look at outer and inner layers of the retina Fundus autofluorescence: shows areas of unhealthy cells in the retina Blood work: the labs
Progressive outer retinal necrosis
Progressive_outer_retinal_necrosis
This manufacturing approach allows the microrafts to possess low autofluorescence and can therefore be utilized for fluorescence-based identification
Microraft
Type of statistical analysis
advantage that there is only signal during a reaction (aside from autofluorescence). In Scanning fluorescence correlation spectroscopy (sFCS) the measurement
Fluorescence correlation spectroscopy
Fluorescence_correlation_spectroscopy
Type of electromagnetic spectroscopy
offering superior contrast over intensity-based methods by minimizing autofluorescence and enabling signal multiplexing. TRFPs emit light after excitation
Fluorescence_spectroscopy
J. W. (May 1985). "Compartmentalization of algal bioluminescence: autofluorescence of bioluminescent particles in the dinoflagellate Gonyaulax as studied
Scintillon
Class of single-celled organisms
A clade F acantharian with symbionts visible in red (chlorophyll autofluorescence)
Acantharia
Measurement of number and characteristics of cells
wavelength than visual light. However, they experienced difficulties with autofluorescence when observing biological material. Fortunately, Köhler saw the potential
Cytometry
Inflammatory condition of the retina of the eye
hypofluorescent lesions in a greater number compared with other studies. Fundus autofluorescence (FAF) has been shown to be a noninvasive method to demonstrate the
Multiple evanescent white dot syndrome
Multiple_evanescent_white_dot_syndrome
Use ultrasound to enhance chemiluminescence
scattering or source instability are absent, and there is no concern about autofluorescence in the background, which can lead to highly sensitive deep tissue imaging
Ultrasound-enhanced chemiluminescence
Ultrasound-enhanced_chemiluminescence
Family of proteins which produce bioluminescence
excitation is not needed for luciferase bioluminescence, there is minimal autofluorescence and therefore the bioluminescent signal is virtually background-free
Luciferase
Czech molecular biologist and astrobiologist
atmosphere of Venus. His research contributed to the development of the autofluorescence nephelometer on the first Morning Star Mission. He is also the inventor
Jan_Špaček
a fluorescent virus-like particle for imaging in tissues with high autofluorescence". Journal of Materials Chemistry B. 11 (20): 4445–4452. doi:10.1039/D3TB00469D
SmURFP
History, technology, medical applications and recent developments of intravascular OCT
Coronary Atherosclerosis With Dual-Modality OCT and Near-Infrared Autofluorescence Imaging". JACC. Cardiovascular Imaging. 9 (11): 1304–1314. doi:10.1016/j
Endoscopic optical coherence tomography imaging
Endoscopic_optical_coherence_tomography_imaging
Genus of single-celled organisms
DIC, images of differential interference contact; Chl, chloroplast autofluorescence; Chl/DAPI, merged images of Chl and DAPI. The double arrowhead indicates
Guillardia
American physicist
October 1998). "Colonic polyp differentiation using time-resolved autofluorescence spectroscopy". Gastrointestinal Endoscopy. 48 (4): 390–394. doi:10
Mary-Ann_Mycek
Genus of bacteria
Two excitation and emission values were found associated with this autofluorescence, which could suggest more than one compound contributes to this unique
Brocadia
autofluorescence found in tissue. However, the use of bright exogenous dyes can provide substantially more remitted light than the autofluorescence approach
Microscopy with UV surface excitation
Microscopy_with_UV_surface_excitation
Metal-free biologically compatible quantum dots with photoluminescence
fluorescence imaging is useful for biological imaging as many tissues exhibit autofluorescence that can interfere with imaging. By using this technique, the signal
Silicon_quantum_dot
Family of single-celled organisms
DIC, images of differential interference contact; Chl, chloroplast autofluorescence; Chl/DAPI, merged images of Chl and DAPI. The double arrowhead indicates
Geminigeraceae
Species of algae
may exist beyond the oocyte and early embryonic stage. Chlorophyll autofluorescence observation and ribosomal DNA analysis suggest that this algal species
Chlorococcum_amblystomatis
Microscope imaging technique
SHG in multiphoton imaging to reveal some molecules that do produce autofluorescence, like elastin in tissues (while SHG reveals collagen or myosin for
Second-harmonic imaging microscopy
Second-harmonic_imaging_microscopy
Fraction of plankton between 0.2 and 2 μm
autotrophs could have photosynthetic pigments and the ability to show autofluorescence, which would allow for their enumeration under epifluorescence microscopy
Picoplankton
568 and 647 nm), enables to gather spectral information about the autofluorescence signal. This has been used for of human eye tissue affected by macular
Vertico spatially modulated illumination
Vertico_spatially_modulated_illumination
Nepenthes benstonei × rafflesiana (Nepenthaceae) emits vivid green autofluorescence: the first report for the genus". Tropical Ecology. 41 (e31) e31: 1–9
List of Nepenthes natural hybrids
List_of_Nepenthes_natural_hybrids
Method of eye examination
May;48(5):2297-303. Morgan JI, Dubra A, Wolfe R, Merigan WH, Williams DR. "In vivo autofluorescence imaging of the human and macaque retinal pigment epithelial cell mosaic
Scanning_laser_ophthalmoscopy
Mass cytometry application to quantify labeled cell targets
antibodies. Some cell types, such as myeloid cells, have high rates of autofluorescence that create a lot of background noise in flow cytometry. However, the
CyTOF
Irish ophthalmologist
Surgery. Lippincott Williams & Wilkins. Lois, Noemi (2012). Fundus Autofluorescence. Lippincott Williams & Wilkins. Lois, Noemi (2013). Diagnostic Technologies
Noemi_Lois
Species of pitcher plant from Southeast Asia
Nepenthes benstonei × rafflesiana (Nepenthaceae) emits vivid green autofluorescence: the first report for the genus". Tropical Ecology. 41 e31: 1–9. doi:10
Nepenthes_rafflesiana
Medical condition
field testing, fundus photography, fluorescein angiography, fundus autofluorescence, and ERG. The differential diagnosis for patients with suspected AZOOR
Acute zonal occult outer retinopathy
Acute_zonal_occult_outer_retinopathy
Photoluminescent particles
narrow emission spectra, high chemical stability, low toxicity, weak autofluorescence background, long luminescence lifetime, and high resistance to photoquenching
Upconverting_nanoparticles
Medical intervention
fluorescence signal from tumor tissue is not very specific and includes autofluorescence from endogenous molecules. Thus, mass spectrometry as an analytical
Fluorescence image-guided surgery
Fluorescence_image-guided_surgery
Autoimmune eye disease
ellipsoid layer and photoreceptor cells, abnormalities in ERG, fundus autofluorescence and visual field defectss. It is a paraneoplastic type of autoimmune
Cancer_associated_retinopathy
Insect protein
unique property of rec1-Resilin is its ability to be identified due to autofluorescence. Fluorescence for resilin stems primarily from dityrosine, which are
Resilin
American biophysicist (1927–2020)
photodamage and minimizes image degradation due to scattering and autofluorescence. His laboratory at Cornell University continues to extend the frontiers
Watt_W._Webb
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