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Type of DNA library
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of
CDNA_library
DNA reverse transcribed from RNA
complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both
Complementary_DNA
Molecular biology technique
amplifying a library of such in bacterial plasmids. In 1982–1983, the idea of selecting random or semi-random clones from such a cDNA library for sequencing
Serial analysis of gene expression
Serial_analysis_of_gene_expression
Collection of genetic material fragments
There are different types of DNA libraries, including cDNA libraries (formed from reverse-transcribed RNA), genomic libraries (formed from genomic DNA) and
Library_(biology)
Brand of data center GPUs by AMD
the CDNA 3 architecture, which is optimized for high-performance computing (HPC) and generative artificial intelligence (AI) workloads. The CDNA 3 architecture
AMD_Instinct
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TRAPPC4
Sub-sequence DNA in genetics
one-shot sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNA library. The resulting sequence is a
Expressed_sequence_tag
Sequencing technique for non-coding RNA
templates, to generate cDNA products for next-generation sequencing (NGS). Overall, OTTR offers a streamlined approach for cDNA library production of full-length
Ordered_two-template_relay
Lock-and-key pairing between two structures
hybrids between RNA and DNA, and opens the door to modern tools such as cDNA libraries. While most complementarity is seen between two separate strings of
Complementarity (molecular biology)
Complementarity_(molecular_biology)
to a company associated with Open Biosystems. complementary DNA (cDNA) cDNA library expressed sequence tags (EST) vector (molecular biology) cloning vector
IMAGE_cDNA_clones
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ZNF274
Protein-coding gene in the species Homo sapiens
1994). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics
SEMA3C
To identify peptides binding a target
peptide library in which each peptide is covalently linked to an mRNA:cDNA which encodes it. The immobilized target is mixed with the library in a binding
MRNA_display
Range of methods designed for assigning cell types
in the film. This method allows further RNA transcript profiling and cDNA library generation of the retrieved cells. RNA sequencing of the selected regions
Spatial_transcriptomics
Protein family
kinase refers to the first cloning of a SGK family member from a cDNA library screen for genes upregulated by the glucocorticoid dexamethasone in a
SGK
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Mitochondrial ribosomal protein L24
Mitochondrial_ribosomal_protein_L24
Lab technique in cellular biology
at a specific time. It enables transcriptome-wide analysis by sequencing cDNA derived from RNA. Modern workflows often incorporate pseudoalignment tools
RNA-Seq
isolated RNA molecules from a tester sample to samples in a control cDNA library. It is a variant of the northern blot in which the nucleic acid immobilized
Reverse_northern_blot
Protein-coding gene in humans
(September 1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10
CHD1L
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CLDN5
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Discoidin domain-containing receptor 2
Discoidin_domain-containing_receptor_2
Type of enzyme
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Lactate_dehydrogenase_A
American biologist
full length double stranded DNA copies of messenger RNA (termed “copy” or cDNA). This method provided a key step in the isolation of human genes, and in
Tom_Maniatis
Parasitic disease caused by a family of nematode worms
to screen against the W.bancrofti Larval stage 3 cDNA library. This led to the identification of a cDNA sequence of W.bancrofti SXP-1 that encoded for a
Filariasis
Technology for examining cells
well as the simultaneous generation of a cDNA library from mRNAs. Next, spatial barcodes are added to the cDNA and gDNA to capture the location of these
Spatial DNA methylation profiling
Spatial_DNA_methylation_profiling
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ZNF451
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
MTCH2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ACSM3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ELP3
Protein involved in the breakdown of blood clots
immunoprecipitation. The resulting cDNA library was subsequently screened via sequence analysis and compared to a whole genome library for confirmation of specific
Tissue-type plasminogen activator
Tissue-type_plasminogen_activator
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GINS2
Medical condition
about 8kb with 13 exon sequences. It was successfully cloned from a cDNA library in 1995 revealing that, after processing, it is 477 nucleotides long
Variegate_porphyria
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
PDCD6
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DNAL4
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
MAGEA3
Nuclear protein inhibiting DNA replication
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Geminin
Mammalian protein found in Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
FCGR2A
Disease causing insufficient blood cells of all types
the serologic screening of a fetal liver library with sera from 8 patients. The human fetal liver cDNA library (chosen because of its high enrichment of
Aplastic_anemia
Mammalian protein found in Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Uroporphyrinogen III decarboxylase
Uroporphyrinogen_III_decarboxylase
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
NPDC1
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Secretoneurin
Protein-coding gene in the species Homo sapiens
5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. IFNGR1+protein,+human at the U.S. National Library of Medicine
Interferon_gamma_receptor_1
Protein-coding gene in the species Homo sapiens
type. Fast skeletal muscle TnI was first cloned from a skeletal muscle cDNA library. It is generally observed that fsTnI is exclusively expressed in fast
Troponin I, fast skeletal muscle
Troponin_I,_fast_skeletal_muscle
Ability of a cell to take up DNA
Cells for High-Efficiency Plasmid Transformation of Escherichia coli". CDNA Library Protocols. Methods in Molecular Biology. Vol. 69. pp. 129–138. doi:10
Transformation_efficiency
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TRIM21
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
EIF3EIP
Enzyme which generates DNA
convert single-stranded RNA into double-stranded cDNA. In retroviruses and retrotransposons, this cDNA can then be endogenized into the host genome, from
Reverse_transcriptase
Protein-coding gene in the species Homo sapiens
Watanabe S, Suzuki F, Yamaguchi N (Oct 1992). "Use of an epitope-tagged cDNA library to isolate cDNAs encoding proteins with nuclear localization potential"
PPIC
Process of isolation and amplification of DNA segments
obtain the cDNA for a specific gene, it is first necessary to construct a cDNA library. The DNA of interest needs to be fragmented to provide a relevant DNA
In_vitro_recombination
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Acylglycerol kinase, mitochondrial
Acylglycerol_kinase,_mitochondrial
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CRHBP
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
EIF4G3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SMTN
Protein domain
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GMP_synthase
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HDHD1A
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
COPS4
high-salt wash, SDS-PAGE, membrane transfer, proteinase digestion, cDNA library preparation and sequencing in order to identify the direct RNA binding
RNA_immunoprecipitation_chip
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
EIF3H
Enzyme of the glycolysis metabolic pathway
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GAPDHS
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GAS7
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CCNDBP1
clones is the cDNA library. This generates a snap-shot of tissue mRNA that is stable and can be sequenced at a later stage. The cDNA library must be sequenced
Digital transcriptome subtraction
Digital_transcriptome_subtraction
nucleotides at the end) to create a cDNA library for each sample, followed by PCR amplification using arbitrary 3′ primers for cDNA strand amplification together
Differential_display
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TREM1
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CDK13
Hypothetical human protein
protein found on chromosome 16. Its protein is 312 amino acids long and its cDNA has 1214 base pairs. It was previously designated C16orf42. The function
TSR3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SERPINB1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SH2D4A
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
INTS9
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
STRN4
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
FLOT1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ME1_(gene)
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HUWE1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SUMO2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
NLRP2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DEPTOR
Protein-coding gene
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TMED10
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DGKZ
assay, a genomic or cDNA library is screened to identify the genetic sequence of a protein of interest. Expression cDNA libraries may be screened with
Functional_cloning
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
FBXO11
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
PEX6
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SART3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
LGTN
transcriptional repression. BAHD1 was first cloned from a human brain cDNA library and the coding sequence was named KIAA0945. Bierne and colleagues further
Bromo adjacent homology domain containing 1
Bromo_adjacent_homology_domain_containing_1
Set of all RNA molecules in one cell or a population of cells
and proteome. The first study to present a case of a collection of a cDNA library for silk moth mRNA was published in 1979. The first seminal study to
Transcriptome
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GTF2H4
Method used in molecular biology
sites that contained the expected Nova-binding motifs. Sequencing of the cDNA library identified many positions close to alternative exons, several of which
Cross-linking immunoprecipitation
Cross-linking_immunoprecipitation
Protein found in humans
play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also
Arrestin_beta_2
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TRMT12
Enzyme found in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
PSMD12
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
LRRC8D
Protein and coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HOMER1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HSF2
Protein found in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
60S acidic ribosomal protein P0
60S_acidic_ribosomal_protein_P0
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
NECAP2
Protein-coding gene in the species Homo sapiens
"disrupted meiotic cDNA" and refers to the method used for its discovery which involved using clones from a meiosis-specific cDNA library to direct knock-out
DNA_meiotic_recombinase_1
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GIT2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SGK3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SNX2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Monocarboxylate_transporter_5
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