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Method of DNA sequencing
the following year by the company Pyrosequencing.[citation needed] A third variant, a microfluidic pyrosequencing method, was described in 2005 by an
Pyrosequencing
DNA sequencing using the concept of massively parallel processing
first next-generation sequencers were based on pyrosequencing, originally developed by Pyrosequencing AB and later commercialized by 454 Life Sciences
Massively_parallel_sequencing
In genetics and biochemistry, determining the structure of an unbranched biopolymer
technologies such as pyrosequencing are gaining an increasing share of the sequencing market. More genome data are now being produced by pyrosequencing than Sanger
Sequencing
Lab procedure detecting 5-methylcytosines in DNA
bisulfite-conversion DNA methylation analysis techniques in general. Pyrosequencing has also been used to analyze bisulfite-treated DNA without using methylation-specific
Bisulfite_sequencing
Process of determining the nucleic acid sequence
Royal Institute of Technology in Stockholm published their method of pyrosequencing. On 1 April 1997, Pascal Mayer and Laurent Farinelli submitted patents
DNA_sequencing
Swedish professor of biochemistry
(former Pyrosequencing AB)" in 1997,[independent source needed] which is more accurately stated as his participating in the founding of a pyrosequencing unit
Pål_Nyrén
Iranian molecular biologist
array-based pyrosequencing.[better source needed] In 1998, Ronaghi, Pål Nyren, and Mathias Uhlen described a solution-based variant of the pyrosequencing technology
Mostafa_Ronaghi
Genetic profiling of embryos prior to implantation
more accessible. Two examples of NGS are the pyrosequencing and the reversible dye terminator. Pyrosequencing technique is based on sequencing by-synthesis
Preimplantation genetic diagnosis
Preimplantation_genetic_diagnosis
Biotechnology company
of the structure of DNA. 454 Sequencing used a large-scale parallel pyrosequencing system capable of sequencing roughly 400-600 megabases of DNA per 10-hour
454_Life_Sciences
Modified polymerase chain reaction protocol
direct sequencing for detection of mutations by Sanger sequencing or pyrosequencing. Replacing traditional PCR with COLD-PCR for these downstream assays
COLD-PCR
Study of genes found in the environment
generated with high-throughput sequencing, in this case massively parallel pyrosequencing developed by 454 Life Sciences. Another early paper in this area appeared
Metagenomics
Family of insects
CS (January 2013). "Surveying the microbiome of ants: comparing 454 pyrosequencing with traditional methods to uncover bacterial diversity". Applied and
Ant
Genus of fungi
in water supply reservoirs are revealed by quantitative PCR and 454 pyrosequencing". BMC Microbiology. 15: 44. doi:10.1186/s12866-015-0379-7. PMC 4349462
Rhodotorula
Species of large land mammal
the white rhinoceros (Ceratotherium simum) as determined by barcoded pyrosequencing analysis". PLOS ONE. 8 (7) e70103. Bibcode:2013PLoSO...870103B. doi:10
White_rhinoceros
Skin condition of the scalp
as P. acnes, and aerobic bacteria, such as Staphylococcus. Using 454 pyrosequencing of the microbiome on scalp dandruff, eleven bacterial phyla were detected
Dandruff
Method of species identification using a short section of DNA
herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures". Frontiers
DNA_barcoding
Discipline in genetics
are added and non-incorporated nucleotides are washed away. Unlike pyrosequencing, the DNA chains are extended one nucleotide at a time and image acquisition
Genomics
Medical condition
cytoplasm does not disclose Birbeck granules. More sensitive methods like pyrosequencing or digital droplet polymerase chain reaction may be helpful if aforementioned
Erdheim–Chester_disease
Enzyme
been employed in the degradation of unincorporated nucleosides during pyrosequencing. The salivary apyrases of blood-feeding arthropods are nucleotide hydrolysing
Apyrase
Species of virus
Bornaviridae, which also contains non-avian bornaviruses. In 2008, by pyrosequencing of cDNA from the brains of several parrots suffering from PDD, Honkavuori
Avian_bornavirus
DNA sequencing method
multiple, expensive enzymes required by other sequencing techniques (i.e. pyrosequencing). CA 2158975, Canard, Bruno & Sarfati, Simon, "Novel derivatives usable
Illumina_dye_sequencing
Emission of light as a result of a chemical reaction
biomolecules in systems such as ELISA and Western blots DNA sequencing using pyrosequencing Lighting objects. Chemiluminescence kites, emergency lighting, glow
Chemiluminescence
Classification by similarity of DNA
H.; Hugenholtz, P. (January 2010). "Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates". Environ
Operational_taxonomic_unit
Species of bacterium
home-made yoghurts of Xinjiang, China by pyrosequencing: Microbial diversity in home-made yoghurts by pyrosequencing". Journal of the Science of Food and
Lactobacillus_kefiranofaciens
Bacterial lab test
its affordability compared to expensive identification methods like pyrosequencing or MALDI-TOF MS that require expensive machines and reagents. The nicotinamide
Niacin_test
Scientific instrument that automates the DNA sequencing process
template strain. Roche currently manufactures two systems based on their pyrosequencing technology: the GS FLX+ and the GS Junior System. The GS FLX+ System
DNA_sequencer
Single-molecule sequencing technology
(US$) Cost of instrument (US$) Run time (h/Gbp) Massively parallel pyrosequencing by synthesis Second 400–500 Q20 read length of 40 bases (99% at 400
Transmission electron microscopy DNA sequencing
Transmission_electron_microscopy_DNA_sequencing
Species of bacterium
Biofilm Infections Determined Using Bacterial Tag Encoded FLX Amplicon Pyrosequencing (bTEFAP)". PLOS ONE. 3 (10) e3326. Bibcode:2008PLoSO...3.3326D. doi:10
Anaerococcus_lactolyticus
Software for DNA sequencing
specifically for assembling sequence data generated by the 454 GS-series of pyrosequencing platforms sold by 454 Life Sciences, a Roche Diagnostics company. Newbler
Newbler
Type of Mesoamerican agriculture
Wang, En Tao (2 October 2015). "Bacterial communities estimated by pyrosequencing in the soils of chinampa, a traditional sustainable agro-ecosystem in
Chinampa
Bioinformatics technique
Goldberg et al. evaluated the effectiveness of combining high throughput pyrosequencing with traditional Sanger sequencing. They were able to greatly increase
Scaffolding_(bioinformatics)
Antibiotic medication
two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing". European Journal of Clinical Microbiology & Infectious Diseases. 27
Linezolid
Application of minerals to soil
"Microbial Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and Phospholipid Fatty Acid Analyses". PLOS ONE. 12 (1) e0168497. Bibcode:2017PLoSO
Liming_(soil)
Organic compound with a –C≡N functional group
Birger Lindberg Møller, Jan Gorodkin, Søren Bak (December 2009), "454 pyrosequencing based transcriptome analysis of Zygaena filipendulae with focus on genes
Nitrile
Sequencing all the DNA of an individual at once
"next-generation") sequencing technologies such as Illumina dye sequencing, pyrosequencing, and SMRT sequencing. All of these technologies continue to employ the
Whole_genome_sequencing
Open source software used in bioinformatics to process DNA sequences
processing data generated from several DNA sequencing methods including 454 pyrosequencing, Illumina HiSeq and MiSeq, Sanger, PacBio, and IonTorrent. The first
Mothur
Distance metric used for comparing biological communities
phylogenetic analyses of microbial communities including analysis of pyrosequencing and Phylo Chip data". The ISME Journal. 4 (1): 17–27. doi:10.1038/ismej
UniFrac
Method of DNA sequencing developed in 1977
PCR-based cloning and next-generation sequencing technologies based on pyrosequencing often avoid using cloning vectors. Recently, one-step Sanger sequencing
Sanger_sequencing
Method of DNA sequence reconstruction
needed large computing farms and distributed computing. By 2004 / 2005, pyrosequencing had been brought to commercial viability by 454 Life Sciences. This
Sequence_assembly
confused with "sequencing by synthesis," a principle used by Roche-454 pyrosequencing (introduced in 2005, generating millions of 200-400bp reads in 2009)
ABI_Solid_Sequencing
Species of bacterium
genus that is positive for staph coagulase. Shotgun and paired-end pyrosequencing were one of the technique using to study the genome of S. capitis. Researchers
Staphylococcus_capitis
Topics referred to by the same term
Standard flowgram format, a binary file format to encode results of pyrosequencing Felts Field (IATA airport code: SFF), an airport near Spokane, Washington
SFF
Mammalian protein found in Homo sapiens
such as methylation-specific polymerase chain reaction (MS-PCR) or pyrosequencing are preferred over immunohistochemical or RNA- based assays. In patients
Methylated-DNA–protein-cysteine methyltransferase
Methylated-DNA–protein-cysteine_methyltransferase
Species of darkling beetle, larval form
reduction in the bacterial community size, which was determined with pyrosequencing of the 16S rRNA gene, and no negative relationship when kanamycin was
Mealworm
Intergenic DNA sequence separating ribosomal RNA genes
Luo, Hongmei; Pang, Xiaohui; Sun, Zhiying (2012-08-30). "Extensive Pyrosequencing Reveals Frequent Intra-Genomic Variations of Internal Transcribed Spacer
Internal_transcribed_spacer
DNA analysis method
profiling of human DNA methylation. Similar to bisulfite sequencing and pyrosequencing, this method quantifies methylation levels at various loci within the
Illumina_Methylation_Assay
Species of bacterium
Huete-Stauffer, Carla; Pruzzo, Carla; Cerrano, Carlo (26 June 2013). "16SrDNA Pyrosequencing of the Mediterranean Gorgonian Paramuricea clavata Reveals a Link among
Endozoicomonas_gorgoniicola
Measure of how acidic or alkaline the soil is
Christian L.; Hamady, Micah; Knight, Rob; Fierer, Noah (August 2009). "Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community
Soil_pH
Genus of single-celled organisms
Fleischer, RC., Carney, KJ., Holzer, KK., Ruiz, Gm. 2016: Amplicon-Based Pyrosequencing Reveals High Diversity of Protistan Parasites in Ships' Ballast Water:
Syndinium
Phylum of bacteria
(2009-01-08). "A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses". The ISME Journal. 3 (4): 442–453. Bibcode:2009ISMEJ
Acidobacteriota
Medical condition
This is specific as it uses the methods of polymerase chain reactions, pyrosequencing and other techniques to detect the parasite’s DNA in samples. It is
Trematodiasis
High resolution melt technique for detection of mutations, polymorphisms in Dna
patients obtained by either MSP, pyrosequencing, and HRM. The HRM method was found to be at least equivalent to pyrosequencing in quantifying the methylation
High-resolution melting analysis
High-resolution_melting_analysis
Species of sponge
(nuclear). Genbank number KC481224 One meta-transcriptome was obtained by pyrosequencing from a specimen collected south of Bergen (Norway) in 2007. Double-stranded
Geodia_barretti
Use of DNA barcoding to analyse the diet of organisms
herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures". Frontiers
DNA barcoding in diet assessment
DNA_barcoding_in_diet_assessment
Protein-coding gene in humans
Salto-Tellez M, et al. (April 2008). "Detection of BRAF V600E mutation by pyrosequencing". Pathology. 40 (3): 295–298. doi:10.1080/00313020801911512. PMID 18428050
BRAF_(gene)
flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.
Standard_flowgram_format
Community of microorganisms
members of the community. Recent studies use shotgun Sanger sequencing or pyrosequencing to recover the sequences of the reads. The reads can then be assembled
Microbiota
Biological process
such as DNA microarrays (MeDIP-chip) or DNA sequencing (MeDIP-seq). Pyrosequencing of bisulfite treated DNA. This is the sequencing of an amplicon made
DNA_methylation
Study of ancient DNA
developed by Margulies et al., employs bead-based emulsion PCR and pyrosequencing, and was found to be powerful in analyses of aDNA because it avoids
Archaeogenetics
Family of transferases enzymes
for substrate binding. ATP sulfurylase is one of the enzymes used in pyrosequencing. Rosenthal E, Leustek T (November 1995). "A multifunctional Urechis
Sulfate_adenylyltransferase
Biological cell regulation mechanisms
by DNA sequencing or by methods developed to quantify SNPs, such as Pyrosequencing (Biotage) or MassArray (Sequenom), measuring the relative amounts of
Regulation_of_gene_expression
Assembly of microorganisms near plants
Analysis of Bacterial Communities in a Potato Field as Determined by Pyrosequencing". PLOS ONE. 6 (8) e23321. Bibcode:2011PLoSO...623321I. doi:10.1371/journal
Plant_microbiome
Species of virus
Zhou; Yijun Zhou; Shengyue Wang (13 May 2010). "Massively Parallel Pyrosequencing-based Transcriptome Analyses of Small Brown Planthopper (Laodelphax
Rice_stripe_virus
American scientist
population diversity by capture-recapture using massively parallel pyrosequencing". Proceedings of the National Academy of Sciences of the United States
Jonathan_J._Juliano
DNA sequencing method
by other techniques that detect single nucleotide additions such as pyrosequencing. Signals generated from a high repeat number are difficult to differentiate
Ion_semiconductor_sequencing
Estimating waterborne bacteria abundance
2017.10.002. Andersson AF, Riemann L, Bertilsson S (February 2010). "Pyrosequencing reveals contrasting seasonal dynamics of taxa within Baltic Sea bacterioplankton
Bacterioplankton counting methods
Bacterioplankton_counting_methods
Species of fungus
characterization of bacteria and fungi in a poultry bioaerosol using pyrosequencing: a new approach". Journal of Occupational and Environmental Hygiene
Aspergillus_ochraceus
1151 (joint) "Accurate determination of microbial diversity from 454 pyrosequencing data", Nature Methods, 6 (9), 639-641 (joint) Smith, Stevie (27 November
Neil_Hall
RNA component
produces reads at rates 50-fold and 12,000-fold less expensive than 454 pyrosequencing and Sanger sequencing, respectively. While cheaper and allowing for
16S_ribosomal_RNA
Measurement of genetic variations
in both DNA strands. Next-generation sequencing technologies such as pyrosequencing sequence less than 250 bases in a read which limits their ability to
SNP_genotyping
Species of virus
viruses in a wild Ugandan red colobus monkey discovered using direct pyrosequencing". PLOS ONE. 6 (4) e19056. Bibcode:2011PLoSO...619056L. doi:10.1371/journal
Simian hemorrhagic fever virus
Simian_hemorrhagic_fever_virus
Malayan ground leech
Kvist, S.; Brugler, M.; Goh, T.; Giribet, G.; Siddall, M. (2014). "Pyrosequencing the salivary transcriptome of Haemadipsa interrupta (Annelida: Clitellata:
Haemadipsa_interrupta
List of plastid genomes whose DNA sequence is known
Farmerie WG, Folta KM, Soltis DE (August 2006). "Rapid and accurate pyrosequencing of angiosperm plastid genomes". BMC Plant Biology. 6: 17. doi:10.1186/1471-2229-6-17
List_of_sequenced_plastomes
Study of RNA transcripts
identified during the early stages of the sequencing process. In Roche's pyrosequencing method, the intensity of emitted light determines the number of consecutive
Transcriptomics_technologies
Sub-field of genomics
provide targeted therapy. Genomics technologies include: DNA sequencing: Pyrosequencing-based sequencers offer a relatively low-cost method to generate sequence
Oncogenomics
Process of soil formation
Flavio A.O.; Farmerie, William G.; Triplett, Eric W. (5 July 2007). "Pyrosequencing enumerates and contrasts soil microbial diversity". The ISME Journal
Soil_formation
American bioinformatician
(2010-04-01). "Identifying and Removing Artificial Replicates from 454 Pyrosequencing Data". Cold Spring Harbor Protocols. 2010 (4) pdb.prot5409. doi:10.1101/pdb
Tracy_Teal
American scientist and entrepreneur
Ronaghi, Stanford Genome Technology Center, $1.8 million (3 years) "Pyrosequencing Array for DNA Sequencing" Ron Kerr, Helmy Eltoukhy, Bambi Francisco
Helmy_Eltoukhy
Species of fungus
Rhizopogon kretzerae and R. salebrosus (Rhizopogonaceae) from 454 shotgun pyrosequencing". Applications in Plant Sciences. 2 (7) 1400029. doi:10.3732/apps.1400029
Rhizopogon_salebrosus
Poisonous species of fungus
been sequenced using a combination of automated Sanger sequencing and pyrosequencing, and the genome sequence information is publicly searchable. The sequence
Amanita_bisporigera
American biologist
sequencing to investigate environmental samples - specifically, they used pyrosequencing to investigate environmental genome sequences in deep mine microbial
Mya_Breitbart
Medical condition
in non-neoplastic lesions such as Reinke's edema. In one study using pyrosequencing, thirty-one of the forty-four non-neoplastic lesions sampled were found
Reinke's_edema
Species of crustacean
A transcriptome of P. hawaiensis was initially generated using the pyrosequencing technique. This resource is freely available for download. Subsequent
Parhyale_hawaiensis
Epidemiology method
identification of HIV-1 transmission in three lawsuits Using Ultra-Deep pyrosequencing and phylogenetic analysis". J Microbiol Immunol Infect. 54 (4): 596–605
Source_attribution
Group of methods in synthetic biology
molecules. In one approach, oligonucleotides are sequenced on the 454 pyrosequencing platform and a robotic system images and picks individual beads corresponding
Artificial_gene_synthesis
Species of bacterium
SKI4 (ATCC 49151). The genome of A. nasoniae was carried out by DNA pyrosequencing and genome assembly. The assembled draft genome was composed of 3, 567
Arsenophonus_nasoniae
Field of study in cancer research
bisulfite restriction analysis (COBRA) Methylation-specific PCR MethyLight Pyrosequencing Restriction landmark genomic scanning Arbitrary primed PCR HELP assay
Cancer_epigenetics
Fungal community in and on an organism
diversity: comparing ARISA fingerprinting with clone sequencing and pyrosequencing". Fungal Ecology. 2 (4): 160–167. Bibcode:2009FunE....2..160G. doi:10
Mycobiome
Molecular biology technique
high-throughput sequencing techniques (next-generation sequencing, i.e. pyrosequencing), hundred thousands or millions of tags can be analyzed simultaneously
Serial analysis of gene expression
Serial_analysis_of_gene_expression
such as further testing or lifestyle management. 454 Life Sciences Pyrosequencing ABI Solid Sequencing Cancer Genome Project Cancer Genome Atlas Caris
Cancer_genome_sequencing
Mammalian protein found in Homo sapiens
cyclin A1 and cytoglobin in oral cancer: quantitative evaluation using pyrosequencing". British Journal of Cancer. 94 (4): 561–8. doi:10.1038/sj.bjc.6602972
Cytoglobin
Mutation that shifts codon alignment
modified by neighboring DNA secondary structure. Sanger sequencing and pyrosequencing are two methods that have been used to detect frameshift mutations,
Frameshift_mutation
Species of insect
AA, Wan L, Dong YC, Li P, Desneux N, White JA, Niu CY (March 2015). "Pyrosequencing reveals a shift in symbiotic bacteria populations across life stages
Bactrocera_dorsalis
T., Ferriera, S., Friedman, R., ... Venter, J. C. (2006). A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies
Hybrid_genome_assembly
Species of flowering plant
root holoparasite Dactylanthus taylorii (Balanophoraceae) from 454 pyrosequencing". American Journal of Botany. 99 (8): e323–e325. doi:10.3732/ajb.1200013
Dactylanthus_taylorii
time. Well-known examples of such DNA sequencing methods include 454 pyrosequencing (introduced in 2005), the Solexa system (introduced in 2006) and the
2_base_encoding
Swedish scientist
incorporated nucleotide to generate sequencing. This was used by the Pyrosequencing method leading to the first massive parallel sequencing instrument (454)
Mathias_Uhlén
Field of study
resistance was shown through monozygotic twin comparisons and bisulfite pyrosequencing to measure the global DNA methylation of Alu repeats in peripheral blood
Epigenetics of diabetes type 2
Epigenetics_of_diabetes_type_2
Species of bacterium
The foregut community samples were then sequenced via 454-amplicon pyrosequencing. After sequencing, the 16S rRNA gene and its V3/V4 region were specifically
Syntrophococcus
American entomologist
(November 2013). "Surveying the microbiome of ants: Comparing 454 pyrosequencing with traditional methods to uncover bacterial diversity". Applied and
Corrie_Moreau
PYROSEQUENCING
PYROSEQUENCING
PYROSEQUENCING
PYROSEQUENCING
Boy/Male
Hindu
Remembrance
Girl/Female
Hindu, Indian
River
Girl/Female
Indian, Telugu
Goddess Saraswati
Boy/Male
Spanish Teutonic
Day.
Girl/Female
English German
Winged.
Surname or Lastname
Dutch and North German
Dutch and North German : from a Germanic personal name composed of bald ‘bold’ + gÄr, gÄ“r ‘spear’.German : habitational name from any of several places called Belgern, near Torgau and in Saxony.English : variant of Bolger.
Boy/Male
Tamil
Naagadatha | நாகதாதா
One of the kauravas
Boy/Male
Native American
Arrow.
Surname or Lastname
English
English : possibly a habitational name from Cleator in Cumbria, named from Old English clǣte ‘burdock’ + Old Norse erg ‘hill pasture’.Possibly an Americanized spelling of North German Klöter, a variant of Klüter, a humorous nickname for a farmer, from Middle Low German klūt(e) ‘clod’.
Boy/Male
Tamil
Nimmit | நீமà¯à®®à®¿à®¤
Destiny
PYROSEQUENCING
PYROSEQUENCING
PYROSEQUENCING
PYROSEQUENCING
PYROSEQUENCING