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Buffer solution commonly used in biological research
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium
Phosphate-buffered_saline
Salt water for medical purposes
following are used: Phosphate buffered saline (PBS) (recipes from Dulbecco = D-PBS, Galfre, Kuchler, Ausubel etc.) TRIS-buffered saline (TBS) (recipes from
Saline_(medicine)
Aequous solution with a physiological pH and isotonic salt concentration
solution (GBSS) Hanks' balanced salt solution (HBSS) (Dulbecco's) Phosphate buffered saline (PBS) Puck's balanced salt solution Ringer's balanced salt solution
Balanced_salt_solution
Aqueous solution of a weak acid and its conjugate base
used in research are kept in a buffer solution, often phosphate buffered saline (PBS) at pH 7.4. In industry, buffering agents are used in fermentation
Buffer_solution
Topics referred to by the same term
(NaH2BO4), a white, odorless, water-soluble chemical compound Phosphate-buffered saline, a buffer solution commonly used in biological research Primer binding
PBS_(disambiguation)
Fluid used for resuscitation after blood loss
animal. Banana bag Intravenous therapy Oral rehydration therapy Phosphate buffered saline (cell culture) Tyrode's solution British national formulary: BNF
Ringer's_lactate_solution
Study of the microscopic anatomy of cells and tissues of plants and animals
fixative for light microscopy is 10% neutral buffered formalin, or NBF (4% formaldehyde in phosphate buffered saline). For electron microscopy, the most commonly
Histology
Solution used to preserve virus specimens
ambient temperature. Chemical components may include saline solution, phosphate-buffered saline (PBS), or fetal bovine serum (FBS). VTM must be sterile
Viral_transport_medium
Topics referred to by the same term
DPBS may refer to: Dulbecco's phosphate-buffered saline, a buffer solution used in biological research DPBS (CONFIG.SYS directive), a configuration directive
DPBS
Chemical compound
DEPC cannot be used with Tris or HEPES buffers. In contrast, it can be used with phosphate-buffered saline or MOPS. A handy rule is that enzymes or
Diethyl_pyrocarbonate
biology, BLOTTO is a blocking reagent made from nonfat dry milk, phosphate buffered saline, and sodium azide. Its name is an almost-acronym of bovine lacto
Blotto_(biology)
Chemical compound
fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline (PBS) on ice for 10 minutes. In histology and pathology specimens
Paraformaldehyde
Gelatinous substance within the umbilical cord
in a cold and sterile environment using a transfer buffer that contains phosphate buffered saline (PBS). Subsequently, the vessels should be eliminated
Wharton's_jelly
Working electrode used in hydrodynamic voltammetry
linear sweep voltammogram of 100 μM dopamine in phosphate buffered saline. Adapted from D. Sopchak, PhD thesis, Case Western Reserve University, 2001
Rotating_disk_electrode
dishes containing nutrient agar or other appropriate medium. Phosphate Buffered Saline (PBS) or other appropriate diluent. Bacterial suspension or homogenate
Miles_and_Misra_method
Microscopic examination of tissue to study and diagnose disease
is 10% neutral buffered formalin (corresponding to 3.7% w/v formaldehyde in neutral buffered water, such as phosphate buffered saline). The tissue is
Histopathology
Method which allows the purification and detection of proteins
Strep-Tactin, a short washing step with a physiological buffer (e.g. phosphate buffered saline, PBS) removes all other host proteins (step 2). This is
Strep-tag
Chemical compound
sulfoxide 30 mg/ml Solubility in ethanol 30 mg/ml Solubility in Phosphate-buffered saline 2 mg/ml (pH 7.2) Acidity (pKa) 15.2±1.0 Hazards GHS labelling:
Triazabicyclodecene
Blood sampling technique
a flat bottomed microtitre plate. The blood is eluted out in phosphate buffered saline containing 0.05% Tween 80 and 0.005% sodium azide, overnight at
Dried_blood_spot
Resin casting of the 3D structure of blood vessels
by perfusing blood vessels with a physiological fluid such as phosphate buffered saline. Subsequently, the blood vessels of interest are filled with resin
Vascular_corrosion_casting
Technique for assessing cell activity
formazan product that has an absorbance maximum at 490 nm in phosphate-buffered saline. The MTS assay is often described as a 'one-step' MTT assay, which
MTT_assay
Chemical substance
application, it is dissolved in Tris-buffered saline or phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between
Polysorbate_20
Naturally occurring glutamate receptor agonist neurotoxin
acid used for the lesioning of rat's brains is kept frozen in a phosphate-buffered Saline Solution at a pH of 7.4, and can be kept for up to a year with
Ibotenic_acid
Human colon cancer cell line
maintain optimal growth. Passaging involved washing cells with phosphate-buffered saline (PBS), briefly detaching them using 0.25% trypsin solution. The
HT-29
Laboratory test for clinically significant alloantibodies
Ficoll-Paque® density gradient. The PBMC's are washed using a phosphate-buffered saline (PBS) and then suspended in tissue culture media in order to keep
Monocyte_monolayer_assay
plastic cell culture labware or it can be diluted in ice-cold phosphate buffered saline or cell culture media prior to dispensing. Due to its heterogenous
Cultrex_BME
Genetic testing technique
chemical fixative like 4% formaldehyde or paraformaldehyde (PFA) in phosphate buffered saline (PBS). This fixative process essentially "preserves" the structural
Fluorescence in situ hybridization
Fluorescence_in_situ_hybridization
Protection against the H1N1/09 virus
centrifugation. The final preparation is suspended in sterile phosphate buffered saline ready for injection. This vaccine mainly contains the killed virus
2009 swine flu pandemic vaccine
2009_swine_flu_pandemic_vaccine
Species of virus
recorded when the diluent used in the test was veronal buffer rather than phosphate-buffered saline. Elution of virus (the hemagglutinin is part of the virion)
Porcine_parvovirus
Neurochemical indicators
neutral pH with 15-2uL 2.5M Tris pH9 Add ELISA buffer (1.5% BSA and 0.05% Tween 20 in phosphate buffered saline) Perform ELISA analysis. The white block represents
Biomarkers of Alzheimer's disease
Biomarkers_of_Alzheimer's_disease
Fungal infection
different oral sites. Oral rinse involves rinsing the mouth with phosphate-buffered saline for 1 minute and then spitting the solution into a vessel that
Oral_candidiasis
Nucleosome occupancy and methylome technique
Dulbecco's phosphate-buffered saline (DPBS), trypsin or dispase (depending on cell type), trypan blue, hemocytometer, lysis buffer, and wash buffer To treat
NOMe-seq
buffers, including phosphate buffered saline (PBS) or Hank's balanced salt solution (HBSS). Add 1-5 μM biliverdin dimethyl ester in media or buffer containing
SmURFP
Vaccine to prevent shingles
consisting of dioleoyl phosphatidylcholine (DOPC) and cholesterol in phosphate-buffered saline solution. Zostavax contains live attenuated varicella-zoster virus
Zoster_vaccine
Anticonvulsant and analgesic medication
solubility in water and DMSO, with a solubility of 20.1 mg/mL in phosphate-buffered saline (PBS, pH 7.5, 25 °C). The molecule has six rotatable bonds and
Lacosamide
Chemical compound
FlAsH is bound to a model tetracysteine-containing peptide in a phosphate-buffered saline at pH 7.4. Generally, FlAsH-EDT2 has 0.1-0.6 fluorescence quantum
FlAsH-EDT2
Medical laboratory test
longer than 3 hours, the blood samples should be diluted in PBS (phosphate buffered saline) before being stored, and the blood samples should not have granulocytes
ELISpot
Species of virus
taken off the grapevine. Cold phosphate buffered saline (PBS) is then rubbed onto the tissue. Cold phosphate buffered saline is a solution that prevents
Grapevine_virus_A
Cytogenetic technique
let air dry overnight. The following day, immerse the slide in phosphate buffered saline (PBS) for several minutes. Transfer the slides into a 4% formaldehyde
Q-FISH
Novel genomic technique
genome in place. Specifically, after a series of washes with phosphate-buffered saline (PBS), cells are pelletted with centrifugation, and then resuspended
Pore-C
Spider toxin
to have greater thermal stability, with ~10% loss after 48h in phosphate-buffered saline at ~55 °C compared to a ~24% loss of PcTx1 and ~38% loss of oxytocin
Π-Theraphotoxin-Hm3a
Soda lake in British Columbia
concentration of dissolved inorganic phosphate in the world. It is a closed-basin lake, which allows it to maintain high salinity and carbonate alkalinity. In
Last_Chance_Lake
Molecular biology technique
order to dilute out the RNA in the solution. Common wash buffers include Phosphate buffered saline (PBS) or a 10% Tween 20 solution. Improper or inadequate
Northwestern_blot
Chemical compound
described. Solubility of the hydrochloride salt: ~5 mg/ml in Phosphate Buffered Saline (PBS) @ pH 7.2; ~10 mg/ml in ethanol & DMF; ~20 mg/ml in DMSO
NBOMe-mescaline
exogenous dye for 10 seconds and then briefly washed in water or phosphate-buffered saline (PBS). The resulting stained specimens generate bright enough
Microscopy with UV surface excitation
Microscopy_with_UV_surface_excitation
Species of bacterium
conditions, and diluted. The diluted solution was then plated with phosphate-buffered saline (PBS), a salt solution used to maintain osmotic balance and wash
Christensenella_minuta
Body of salt water covering most of Earth
surface salinity between 1950 and 2019 indicate that regions of high salinity and evaporation have become more saline while regions of low salinity and more
Ocean
albumin to form bio-conjugates which were highly dispersed in phosphate-buffered saline and could be taken up by cultured mammalian cells via an endocytosis
Single-walled_carbon_nanohorn
Technique to count and size particles
an electrical current. The salinity levels of seawater or of a wide range of concentrations of phosphate-buffered saline are easily sufficient for this
Resistive_pulse_sensing
Difference in quantities of cations and anions in blood or urine
myeloma).[citation needed] Similarly, tests do often measure the anion phosphate (PO3− 4) specifically, but it isn't used to calculate that "gap," even
Anion_gap
Biogeochemical cycle
membranes). It also functions as a buffering agent in maintaining acid base homeostasis in the human body. Phosphates move quickly through plants and animals;
Phosphorus_cycle
Medical condition
intravenous insulin under observation for signs of deterioration. Normal saline (0.9% saline) has generally been the fluid of choice. There have been a few small
Diabetic_ketoacidosis
Solid, liquid or gel used to grow microorganisms or cells
neutralize. Certain bacterial inhibitors are used for gonococci, and buffered glycerol saline for enteric bacilli. Venkataraman Ramakrishna (VR) medium is used
Growth_medium
and the cover slips were rinsed in buffer-incubated in acid phosphate medium (15 minutes). Naphthol AS TR phosphate was used as the substrate and hexazonium
Identification_of_cell_death
Buffer zone between terrestrial and marine ecosystems
ponds form a vital buffer zone between terrestrial and marine ecosystems. Contaminants such as sediment, nitrates and phosphates are filtered out by
Tropical_salt_pond_ecosystem
Preservation of soil nutrients
prevention of reduced fertility caused by over usage, acidification, salinization or other chemical soil contamination. Slash-and-burn and other unsustainable
Soil_conservation
Landlocked body of water with underground connection to the sea
brackish, and saline water intruding from the coast at depth. Depending on the site, it is sometimes possible to access the deeper saline water directly
Anchialine_system
Aspect of marine biology
and chemical properties of the water. Estuaries have large variation in salinity, ranging from entirely fresh water upstream to fully marine water at the
Shell_growth_in_estuaries
Medical condition
Reddy, Sumeet; Bellomo, Rinaldo (2015-10-27). "Effect of a Buffered Crystalloid Solution vs Saline on Acute Kidney Injury Among Patients in the Intensive
Hyperchloremia
Process of introducing nucleic acids into eukaryotic cells
phosphate, originally discovered by F. L. Graham and A. J. van der Eb in 1973 (see also). HEPES-buffered saline solution (HeBS) containing phosphate ions
Transfection
Type of membrane
of uniformly arranged phospholipids. Each phospholipid is made of one phosphate head and two fatty acid tails. The plasma membrane that surrounds all
Semipermeable_membrane
Preservation of biological tissue
is usually used as a 10% neutral buffered formalin (NBF), that is approx. 3.7%–4.0% formaldehyde in phosphate buffer, pH 7. Since formaldehyde is a gas
Fixation_(histology)
Pond on Sint Maarten in the Dutch Caribbean
directly into the lake, leading to high levels of phosphates and low oxygen levels. Although the low salinity of Fresh Pond allows it to harbour species not
Fresh_Pond,_Sint_Maarten
Imbalance in the body's acid-base equilibrium
acidosis Hyperalimentation Addison disease Acetazolamide Spironolactone Saline infusion To distinguish between the main types of metabolic acidosis, a
Metabolic_acidosis
Fluid injection into the large intestine
body. Sodium phosphate. Also known by the brand name Fleet. Available at drugstores; usually self-administered. Buffered sodium phosphate solution draws
Enema
Crystalline chemical element or compound formed by geologic processes
silicates (e.g. orthoclase KAlSi3O8), molybdates (e.g. wulfenite PbMoO4) and phosphates (e.g. pyromorphite Pb5(PO4)3Cl). The International Mineralogical Association
Mineral
Anti-inflammatory medication
"General chemistry online: FAQ: Acids and bases: What is the buffer system in buffered aspirin?". Antoine.frostburg.edu. Archived from the original on
Aspirin
Environmental problems from uncontrolled mining
which contributes to climate change. Some mining methods (lithium mining, phosphate mining, coal mining, mountaintop removal mining, and sand mining) may
Environmental impact of mining
Environmental_impact_of_mining
Capacity of water to resist changes in pH that would make the water more acidic
components that can contribute to alkalinity include borate, hydroxide, phosphate, silicate, dissolved ammonia, and the conjugate bases of organic acids
Alkalinity
Abnormality in the concentration of electrolytes in the body
with a chloride anion. Other forms of anions are for example chloride phosphate ions) and are anions (negatively− charged). Electrolytes cross barriers
Electrolyte_imbalance
Chemical compound
is used therapeutically as phosphate binder in patients on maintenance haemodialysis. It is the most common form of phosphate binder prescribed, particularly
Calcium_carbonate
Earth, a natural material
underlying water table. Rapid salination occurs when the land surface is within the capillary fringe of saline groundwater. Soil salinity control involves watertable
Soil
Natural harbor on the island of Luzon, Philippines
average surface salinity of 32.6 psu (practical salinity unit). Due to the river influx, the areas near the coasts have low salinity especially in the
Manila_Bay
Liquid by-product of animal metabolism
compared to dry manufactured nitrogen fertilizers such as diammonium phosphate, the relative transport costs for urine are high as a lot of water needs
Urine
Port city in Tunisia
has a population of 341,999 (census 2022). Its main industries include phosphate, olive and nut processing, fishing (it is the largest fishing port in
Sfax
Measure of the level of acidity or basicity of an aqueous solution
Dickson, A. G. (1984). "pH scales and proton-transfer reactions in saline media such as sea water". Geochim. Cosmochim. Acta. 48 (11): 2299–2308.
PH
Branch of environmental chemistry
chemical contaminants. pH is only likely to become an issue on very poorly buffered upland rivers where atmospheric sulphur and nitrogen oxides may very significantly
Freshwater environmental quality parameters
Freshwater_environmental_quality_parameters
Medical intervention
microspheres and non-animal stabilized sodium hyaluronate (NASHA) in phosphate-buffered 0.9% sodium chloride solution. The particles are 120 μm in diameter
Perianal injectable bulking agent
Perianal_injectable_bulking_agent
Historic oxygen depletion events in Earth's oceans
stratification could develop. Under anoxic to euxinic conditions, oceanic phosphate is not retained in sediment and could hence be released and recycled,
Anoxic_event
Chemical compound
fertilisers in various forms and composition, such as urea and diammonium phosphate. Ammonia in pure form is also applied directly into the soil. Ammonia
Ammonia
Marine aquarium that displays live corals and other marine animals
week. In particular, ammonia, nitrite, nitrate, pH, salinity, alkalinity, calcium and phosphate levels should be monitored closely. When it comes to
Reef_aquarium
Marine invertebrates of the subphylum Anthozoa
benefit from a safe place to live and consume the polyp's carbon dioxide, phosphate and nitrogenous waste. Stressed corals will eject their zooxanthellae
Coral
Organ preservation technique
He also compared various perfusate compositions and found that a phosphate buffered perfusate could be used successfully, so eliminating the need for
Machine_perfusion
Species of flightless bird
total phosphate concentration in embryonic stages, but the IPP concentration jumps to 60% of total phosphate of the cell. The majority of phosphate concentration
Common_ostrich
Class of salt-tolerant archaea
geranylgeranylglyceryl phosphate synthase-like protein. Haloarchaea require salt concentrations in excess of 2 mol/L (or about 10%, three times the ocean salinity which
Haloarchaea
Island). This island is a bird sanctuary. Its surroundings are highly phosphatized that superphosphate materials can be mined out on a small-scale basis
List of maritime features in the Spratly Islands
List_of_maritime_features_in_the_Spratly_Islands
secured a license to operate a mobile phone service in Syria and access to phosphate mining in the country's eastern regions. Additionally, Iran will receive
Economic activities of the Iranian Revolutionary Guard Corps
Economic_activities_of_the_Iranian_Revolutionary_Guard_Corps
Red blood cells separated for blood transfusion
solutions are typically based on saline. They usually contain glucose, adenine, mannitol and, sometimes, phosphate and guanosine. The additive solution
Packed_red_blood_cells
Measure of how acidic or alkaline the soil is
profile: certain profile characteristics can be indicators of either acid, saline, or sodic conditions. Examples are: Poor incorporation of the organic surface
Soil_pH
Crop planted to manage erosion and soil quality
increase the availability of phosphorus in soil after a farmer applies rock phosphate. Cover crops can also improve soil quality by increasing soil organic
Cover_crop
Salt water aquarium
invertebrates. Salinity should therefore be between 28 and 35 ppt, with the higher values being beneficial in advanced reef systems. Because salinity is by definition
Marine_aquarium
Capacity of a substance to dissolve in a homogeneous way
doi:10.1063/1.1727043 Tomlinson, Charles (1868-01-01). "On Supersaturated Saline Solutions". Philosophical Transactions of the Royal Society of London. 158
Solubility
Phenomenon where corals expel algae tissue
caused by various stressors, such as changes in water temperature, light, salinity, or nutrients. A bleached coral is under stress, more vulnerable to starvation
Coral_bleaching
Symbiotic penetrative association between a fungus and the roots of a vascular plant
and degraded and may buffer the intracellular sugar concentrations. The intraradical hexose enters the oxidative pentose phosphate pathway, which produces
Arbuscular_mycorrhiza
Synthesis of organic compounds from carbon dioxide by biological organisms
primary production by nutrients. Inorganic nutrients, such as nitrate, phosphate and silicic acid are necessary for phytoplankton to synthesise their cells
Primary_production
Amine hormone
Subsequently, 5-HTP undergoes decarboxylation, facilitated by pyridoxal phosphate and the enzyme 5-hydroxytryptophan decarboxylase, yielding serotonin.
Melatonin
Type of sedimentary rock
aragonite may be suppressed by the presence of naturally occurring organic phosphates in the water. Although ooids likely form through purely inorganic processes
Limestone
Albumin found in human blood
is no strong medical evidence that albumin administration (compared to saline) saves lives for people who have hypovolaemia or for those who are critically
Human_serum_albumin
Population explosion of organisms that can kill marine life
common nutrients are fixed nitrogen (nitrates, ammonia, and urea) and phosphate. The excess nutrients are emitted by agriculture, industrial pollution
Harmful_algal_bloom
Estimating waterborne bacteria abundance
(FDC), thymidine incorporation, and leucine incorporation. Factors such as salinity, temperature, latitude, various nutrient levels, water movement and the
Bacterioplankton counting methods
Bacterioplankton_counting_methods
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PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
Girl/Female
Tamil
Prasad offered to God
Girl/Female
Indian
Offered to God
Girl/Female
Hindu
Dedicated to service, Surrendered, Offered to God
Girl/Female
Tamil
Dedicated to service, Surrendered, Offered to God
Girl/Female
Assamese, Gujarati, Hindu, Indian, Kannada, Malayalam, Marathi, Oriya, Sanskrit, Tamil, Telugu
Dedicated to Service; Surrendered; Offered to God; Requestful
Girl/Female
African, Hindu, Indian, Tamil
Leaves Offered to Ganesha; Cold; Peace
Girl/Female
Bengali, Hindu, Indian, Marathi, Sanskrit
Holy Leaf Offered to Lord Shiva; Auspicious Fruit; A Sacred Leaf
Boy/Male
Tamil
Agnihotra | அகà¯à®¨à®¿à®¹à¯‹à®¤à¯à®°
Sacrifice offered to Agni
Agnihotra | அகà¯à®¨à®¿à®¹à¯‹à®¤à¯à®°
Girl/Female
Biblical
Given or offered.
Boy/Male
Indian, Sanskrit
Food Offered to the Gods; Lord Shiva
Boy/Male
Indian, Sanskrit
Offered by the Sun; Sacred
Boy/Male
Indian, Sanskrit
To be Offered as Oblation; Perpetual Fire
Biblical
given or offered
Girl/Female
Hindu, Indian
Prasad Offered to God
Girl/Female
Hindu, Indian
Man who Offered Service to Lord Rama
Surname or Lastname
English
English : variant of Buford.
Boy/Male
Indian
Sacrifice offered to Agni
Girl/Female
Hindu, Indian, Kannada, Sindhi, Tamil, Telugu
Offered to God
Boy/Male
Hindu, Indian, Marathi
Offered of the Soma Sacrifice
Girl/Female
Hindu, Indian
Holy Leaf Offered to Lord Shiva; A Sacred Leaf
PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
PHOSPHATE BUFFERED-SALINE
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