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CRYOMICROSCOPY

  • Cryo-electron microscopy
  • Electron microscopy technique

    is called transmission electron cryomicroscopy of vitreous sections (CEMOVIS) or transmission electron cryomicroscopy of frozen-hydrated sections. In

    Cryo-electron microscopy

    Cryo-electron microscopy

    Cryo-electron_microscopy

  • Scanning electron cryomicroscopy
  • Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron

    Scanning electron cryomicroscopy

    Scanning_electron_cryomicroscopy

  • Cryomicroscopy
  • Microscopy below room temperature

    Cryomicroscopy is a technique in which a microscope is equipped in such a fashion that the object intended to be inspected can be cooled to below room

    Cryomicroscopy

    Cryomicroscopy

  • Solaris (synchrotron)
  • Synchrotron light source in Kraków, Poland

    and worked in Kraków. On March 1, 2019, at the First Congress of the Cryomicroscopy Consortium, a body gathering specialists in structural biology from

    Solaris (synchrotron)

    Solaris (synchrotron)

    Solaris_(synchrotron)

  • Cryogenic electron tomography
  • Three-dimensional imaging technique

    cells. cryoET is a specialized application of transmission electron cryomicroscopy (CryoTEM) in which samples are imaged as they are tilted, resulting

    Cryogenic electron tomography

    Cryogenic electron tomography

    Cryogenic_electron_tomography

  • Humberto Fernández-Morán
  • Venezuelan scientist (1924–1999)

    units are possible. He also helped to advance the field of electron cryomicroscopy - the use of superconductive electromagnetic lenses cooled with liquid

    Humberto Fernández-Morán

    Humberto Fernández-Morán

    Humberto_Fernández-Morán

  • List of Greek and Latin roots in English/A–G
  • All Latin and Greek roots beginning with G

    cryobiology, cryobot, cryochemistry, cryoelectronics, cryogenics, cryomicroscopy, cryoneurolysis, cryonics, cryopreservation, cryoprotectant, cryosphere

    List of Greek and Latin roots in English/A–G

    List_of_Greek_and_Latin_roots_in_English/A–G

  • Electron microscope
  • Type of microscope with electrons as a source of illumination

    (2010-01-01), Jensen GJ (ed.), "Chapter Fifteen - Radiation Damage in Electron Cryomicroscopy", Methods in Enzymology, Cryo-EM Part A Sample Preparation and Data

    Electron microscope

    Electron microscope

    Electron_microscope

  • Coronavirus
  • Subfamily of viruses in the family Coronaviridae

    severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy". Journal of Virology. 80 (16): 7918–28. doi:10.1128/JVI.00645-06. PMC 1563832

    Coronavirus

    Coronavirus

    Coronavirus

  • Oxidative phosphorylation
  • Metabolic pathway

    (December 2003). "Structure of the mitochondrial ATP synthase by electron cryomicroscopy". The EMBO Journal. 22 (23): 6182–6192. doi:10.1093/emboj/cdg608. PMC 291849

    Oxidative phosphorylation

    Oxidative phosphorylation

    Oxidative_phosphorylation

  • Jacques Dubochet
  • Swiss biophysicist

    2016). "A Reminiscence about Early Times of Vitreous Water in Electron Cryomicroscopy". Biophysical Journal. 110 (4): 756–757. Bibcode:2016BpJ...110..756D

    Jacques Dubochet

    Jacques Dubochet

    Jacques_Dubochet

  • Fatty acid synthase
  • Class of enzymes

    substrate-shuttling mechanism of yeast fatty acid synthase by electron cryomicroscopy". Proceedings of the National Academy of Sciences of the United States

    Fatty acid synthase

    Fatty acid synthase

    Fatty_acid_synthase

  • Piscine orthoreovirus
  • Species of virus

    2003). "Cytoplasmic polyhedrosis virus structure at 8 A by electron cryomicroscopy: structural basis of capsid stability and mRNA processing regulation"

    Piscine orthoreovirus

    Piscine orthoreovirus

    Piscine_orthoreovirus

  • SnRNP
  • Protein complexes that mix with other proteins to form spliceosome

    Integration into the Spliceosomal U11/U12 di-snRNP as Revealed by Electron Cryomicroscopy". Molecular Cell. 17 (6): 869–883. doi:10.1016/j.molcel.2005.02.016

    SnRNP

    SnRNP

  • Andrea Musacchio
  • Italian biologist

    "Functional Organization of Clathrin in Coats: Combining Electron Cryomicroscopy and X-Ray Crystallography". Molecular Cell. 3 (6): 761–70. doi:10

    Andrea Musacchio

    Andrea Musacchio

    Andrea_Musacchio

  • Proteasome
  • Protein complexes which degrade ubiquitin-tagged proteins by proteolysis

    of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy". Proceedings of the National Academy of Sciences. 109 (5): 1479–1484

    Proteasome

    Proteasome

    Proteasome

  • SARS-related coronavirus
  • Species of coronavirus causing SARS and COVID-19

    severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy". Journal of Virology. 80 (16): 7918–28. doi:10.1128/JVI.00645-06. PMC 1563832

    SARS-related coronavirus

    SARS-related coronavirus

    SARS-related_coronavirus

  • Marina Rodnina
  • German biochemist (born 1960)

    (2010). "Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy". Nature. 466 (7304): 329–333. doi:10.1038/nature09206. PMID 20631791

    Marina Rodnina

    Marina_Rodnina

  • List of Greek and Latin roots in English/C
  • All Latin and Greek roots beginning with C

    cryobiology, cryobot, cryochemistry, cryoelectronics, cryogenics, cryomicroscopy, cryoneurolysis, cryonics, cryopreservation, cryoprotectant, cryosphere

    List of Greek and Latin roots in English/C

    List_of_Greek_and_Latin_roots_in_English/C

  • Double-stranded RNA viruses
  • Type of virus according to Baltimore classification

    family, Rice Dwarf Virus (RDV), has been extensively studied by electron cryomicroscopy and x-ray crystallography. From these analyses, atomic models of the

    Double-stranded RNA viruses

    Double-stranded RNA viruses

    Double-stranded_RNA_viruses

  • Structural biology
  • Study of molecular structures in biology

    high-resolution three-dimensional structure of bacteriorhodopsin using electron cryomicroscopy. Subsequent advances in electron detectors, image processing and three-dimensional

    Structural biology

    Structural biology

    Structural_biology

  • Clathrin
  • Protein playing a major role in the formation of coated vesicles

    2004). "Molecular model for a complete clathrin lattice from electron cryomicroscopy". Nature. 432 (7017): 573–579. Bibcode:2004Natur.432..573F. doi:10.1038/nature03079

    Clathrin

    Clathrin

    Clathrin

  • Inositol trisphosphate receptor
  • Class of transport proteins

    (November 2015). "Gating machinery of InsP3R channels revealed by electron cryomicroscopy". Nature. 527 (7578): 336–341. Bibcode:2015Natur.527..336F. doi:10.1038/nature15249

    Inositol trisphosphate receptor

    Inositol trisphosphate receptor

    Inositol_trisphosphate_receptor

  • Apoptosome
  • Protein complex involved in the cellular apoptotic process

    three-dimensional structure was first determined at 27 Å resolution using electron cryomicroscopy, with a calculated mass of 1 megadalton (Mda) (Acehan et al. 2002).

    Apoptosome

    Apoptosome

    Apoptosome

  • Fourier shell correlation
  • orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy". Journal of Molecular Biology. 333 (4): 721–745. doi:10.1016/j.jmb

    Fourier shell correlation

    Fourier_shell_correlation

  • Eukaryotic ribosome
  • Large molecular machine for synthesizing proteins from messenger RNA

    (Mar 1998). "The 80S rat liver ribosome at 25 A resolution by electron cryomicroscopy and angular reconstitution". Structure. 6 (3): 389–399. doi:10

    Eukaryotic ribosome

    Eukaryotic ribosome

    Eukaryotic_ribosome

  • Fatty-acyl-CoA synthase
  • substrate-shuttling mechanism of yeast fatty acid synthase by electron cryomicroscopy". Proc. Natl. Acad. Sci. U.S.A. 107 (20): 9164–9. Bibcode:2010PNAS.

    Fatty-acyl-CoA synthase

    Fatty-acyl-CoA synthase

    Fatty-acyl-CoA_synthase

  • Partitiviridae
  • Family of viruses

    (2010). "Backbone Trace of Partitivirus Capsid Protein from Electron Cryomicroscopy and Homology Modeling". Biophysical Journal. 99 (2): 685–694. Bibcode:2010BpJ

    Partitiviridae

    Partitiviridae

    Partitiviridae

  • Salmonella virus Epsilon15
  • Species of virus

    "Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy". Nature. 451 (7182): 1130–4. Bibcode:2008Natur.451.1130J. doi:10.1038/nature06665

    Salmonella virus Epsilon15

    Salmonella_virus_Epsilon15

  • Icosahedral twins
  • Structure found in atomic clusters and nanoparticles

    "Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy". Nature. 386 (6620): 88–91. Bibcode:1997Natur.386...88B. doi:10.1038/386088a0

    Icosahedral twins

    Icosahedral twins

    Icosahedral_twins

  • Sean Whelan (scientist)
  • British-American virologist

    "Structure of the L Protein of Vesicular Stomatitis Virus from Electron Cryomicroscopy". Cell. 162 (2): 314–327. doi:10.1016/j.cell.2015.06.018. ISSN 1097-4172

    Sean Whelan (scientist)

    Sean Whelan (scientist)

    Sean_Whelan_(scientist)

  • Cryofixation
  • Specimen preparation process

    containing specimens in amorphous ice is the "holy grail" of biological cryomicroscopy.[citation needed] In practice, it is very difficult to achieve high

    Cryofixation

    Cryofixation

  • Single particle analysis
  • Method of analyzing transmission electron microscopy imagery

    negatively stained and vitreous ice-embedded transmission electron cryomicroscopy (CryoTEM) samples. Single particle analysis methods are, in general

    Single particle analysis

    Single particle analysis

    Single_particle_analysis

  • L. E. Scriven
  • apparatus that allows fast freezing of complex liquid specimens for cryomicroscopy (Journal of Electron Microscopy Technique, 1988). His 1988 paper "Physics

    L. E. Scriven

    L._E._Scriven

  • Resolution (structural biology)
  • Orientation, Absolute Hand, and Contrast Loss in Single-particle Electron Cryomicroscopy". Journal of Molecular Biology. 333 (4): 721–745. doi:10.1016/j.jmb

    Resolution (structural biology)

    Resolution_(structural_biology)

  • Phytoreovirus
  • Genus of viruses

    family, Rice dwarf virus (RDV), has been extensively studied by electron cryomicroscopy and x-ray crystallography. From these analyses, atomic models of the

    Phytoreovirus

    Phytoreovirus

    Phytoreovirus

  • Lori Passmore
  • Canadian/British scientist

    (2014). "Electron microscopy: Ultrastable gold substrates for electron cryomicroscopy". Science. 346 (6215): 1377–80. doi:10.1126/science.1259530. PMC 4296556

    Lori Passmore

    Lori Passmore

    Lori_Passmore

  • N-ethylmaleimide sensitive fusion protein
  • Protein-coding gene in Homo sapiens

    Sutton RB, Chen J, Brunger AT, Grigorieff N (Sep 2003). "Electron cryomicroscopy structure of N-ethyl maleimide sensitive factor at 11 A resolution"

    N-ethylmaleimide sensitive fusion protein

    N-ethylmaleimide sensitive fusion protein

    N-ethylmaleimide_sensitive_fusion_protein

  • Electron crystallography
  • Method to determine atomic positions in solids using an electron microscope

    fewer atoms. One technique used to limit radiation damage is electron cryomicroscopy, in which the samples undergo cryofixation and imaging takes place at

    Electron crystallography

    Electron_crystallography

  • Robert Tampé
  • German biochemist

    Tampé R, Craik CS, Cheng Y (2015): Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter. Nature 517, 396–400. doi:10

    Robert Tampé

    Robert Tampé

    Robert_Tampé

  • Bacterial translation
  • Protein synthesis process in bacteria

    of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy". Structure. 18 (6): 719–24. doi:10.1016/j.str.2010.02.017. PMID 20541509

    Bacterial translation

    Bacterial translation

    Bacterial_translation

  • PSMD4
  • Enzyme found in humans

    of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy". Proceedings of the National Academy of Sciences of the United States

    PSMD4

    PSMD4

    PSMD4

  • Paul S. Freemont
  • Structural and synthetic biologist

    conformations of the protein complex p97-Ufd1-Npl4 revealed by electron cryomicroscopy". Proceedings of the National Academy of Sciences of the United States

    Paul S. Freemont

    Paul_S._Freemont

  • Judy Hirst
  • Physical Chemist

    fundamental and medical importance, and for solving its structure by electron cryomicroscopy. Hirst, Judy (1997). Electron transport in redox enzymes. bodleian.ox

    Judy Hirst

    Judy_Hirst

  • DiaGrid (distributed computing network)
  • "Backbone structure of the infectious e15 virus capsid revealed by electron cryomicroscopy" (PDF). Nature. 451 (7182): 1130–1134. Bibcode:2008Natur.451.1130J.

    DiaGrid (distributed computing network)

    DiaGrid_(distributed_computing_network)

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