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Method for cloning recombinant DNA
The T7 expression system is utilized in the field of microbiology to clone recombinant DNA using strains of E. coli. It is the most popular system for
T7_expression_system
Class of enzymes
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction. T7 polymerase is
T7_RNA_polymerase
Strain of bacteria
strain that uses a T7 expression system driven by the tightly regulated arabinose-inducible PBAD promoter. It fixes the leaky expression issue seen in BL21
Escherichia_coli_BL21(DE3)
Virus or plasmid designed for gene expression in cells
Studier FW (1991). "Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor". Journal
Expression_vector
DNA molecules formed by human agency at a molecular level generating novel DNA sequences
Host cell protein T7 expression system Rosano, Germán L.; Ceccarelli, Eduardo A. (17 April 2014). "Recombinant protein expression in Escherichia coli:
Recombinant_DNA
Species of virus
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these
T7_phage
Biotechnological process
prophage found in BL21(DE3) provides T7 RNA polymerase (driven by the LacUV5 promoter), allowing for vectors with the T7 promoter to be used instead. Non-pathogenic
Protein_production
American biochemist
Kathleen S. (2000). "Generation of an AraC-araBAD Promoter-Regulated T7 Expression System". Analytical Biochemistry. 277 (1): 67–73. doi:10.1006/abio.1999
Kathleen Matthews (biochemist)
Kathleen_Matthews_(biochemist)
Division of the autonomic nervous system
parasympathetic nervous system (PSNS) is one of the three divisions of the autonomic nervous system, the others being the sympathetic nervous system and the enteric
Parasympathetic nervous system
Parasympathetic_nervous_system
Group of open reading frames under the same regulation
bacteriophages. For example, T7 phages have two operons. The first operon codes for various products, including a special T7 RNA polymerase which can bind
Operon
Technique for the automated directed evolution of proteins
soluble expression, the N-terminus of a T7 polymerase was fused to APOBEC1, with the remaining portion of the polymerase separately expressed. The T7 polymerase
Phage-assisted continuous evolution
Phage-assisted_continuous_evolution
Part of the autonomic nervous system that stimulates fight-or-flight responses
sympathetic nervous system (SNS; or sympathetic autonomic nervous system, SANS, to differentiate it from the somatic nervous system) is one of the three
Sympathetic_nervous_system
Enzyme
T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands
T7_DNA_polymerase
Nerve system for sensing touch, temperature, body position, and pain
nervous system. All afferent touch/vibration information ascends the spinal cord via the dorsal column-medial lemniscus pathway via gracilis (T7 and below)
Somatosensory_system
Biological technique to evolve proteins using bacteriophages
PMID 21390283. Danner S, Belasco JG (November 2001). "T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries"
Phage_display
Technique in molecular biology
and aptamers. In these systems, pools of randomized or mutagenized DNA sequences are linearized and transcribed in vitro using T7 RNA polymerase to produce
Run-off_transcription
Study of rhythms in biological processes of living organisms
total sleep and their circadian expression of PER2, an element of the SCN pacemaker, was not disrupted. Through the T7 cycle, the mice were exposed to
Chronobiology
Templates in computer programming
more components, the final parameter TRest of Tuple<T1, T2, T3, T4, T5, T6, T7, TRest> is supplied as another tuple, as C# lacks variadic template parameters
Variadic_template
Result of multiplying types in type theory
T2, T3, T4, T5, T6, T7, TRest> is supplied as another tuple. For iterating over collections like dictionary types, the class System.Collections.Generic
Product_type
Tetracycline-class antibiotic
experiments in eukaryotic cells and organisms with inducible protein expression systems using tetracycline-controlled transcriptional activation. The mechanism
Doxycycline
Instruction set architecture
(RISC) instruction set architectures (ISA) developed by MIPS Computer Systems, now MIPS Technologies, based in the United States. There are multiple
MIPS_architecture
Antimicrobial enzyme produced by animals
tool in the expression of toxic recombinant proteins. Expressing recombinant proteins in BL21(DE3) strains is typically accomplished by the T7-RNA-polymerase
Lysozyme
Artificial peptide attached to protein for marking purpose
streptactin (Strep-tag II: WSHPQFEK) T7-tag, an epitope tag derived from the T7 major capsid protein of the T7 gene (MASMTGGQQMG). Used in different
Protein_tag
Imaging technique for in vitro protein
library has a T7 RNA polymerase transcription site and a ribosomal binding site at the 5' end. The T7 promoter region allows large-scale in vitro T7 transcription
MRNA_display
Virus that infects bacteria
phage P4 phage R17 phage T2 phage T4 phage (169 kbp genome, 200 nm long) T7 phage T12 phage Phagesdb Phagescope Viruses portal Antibiotic Bacterivore
Bacteriophage
Rod-shaped, gram-negative bacterium
for the production of heterologous proteins, and various protein expression systems have been developed which allow the production of recombinant proteins
Escherichia_coli
American biochemist
bacteriophage T7 RNA polymerase in 1985. Richardson used the T7 RNA polymerase/promoter system to control the expression of a phage T7 gene 5 protein
Charles_C._Richardson
Purported geroprotective agent
Elashkina EV, Durnova AO (May 2014). "Short peptides stimulate serotonin expression in cells of brain cortex". Bull Exp Biol Med. 157 (1): 77–80. doi:10
Pinealon
Small piece of maintainable DNA
proteins are only produced when required. Some commonly used promoters are the T7 and lac promoters. The presence of a promoter is necessary when screening
Cloning_vector
Proteins performing more than one function
bacteriophage T7, E. coli thioredoxin forms a complex with T7 DNA polymerase, which results in enhanced T7 DNA replication, a crucial step for successful T7 infection
Protein_moonlighting
DNA structure
triplex DNA formation impairs the expression of intron 1 of the FXN gene. This results in the degeneration of the nervous system and spinal cord, impairing the
Triple-stranded_DNA
Austro-American scientist
Gerngross TU (December 2004). "High level recombinant protein expression in Ralstonia eutropha using T7 RNA polymerase based amplification". Protein Expr. Purif
Tillman_Gerngross
Species of Gram-positive bacterium
the interaction of the three membrane proteins with each other and EssC. T7 dependent effector proteins EsaD is DNA endonuclease toxin secreted by S.
Staphylococcus_aureus
Chinese biotechnology company
minutes to read a genome. The company said at a launch event that the DNBSEQ-T7+ can sequence 144 human genomes a day, or one every 10 minutes. On October
MGI_(company)
DNA construct
resistance; some BACs also have lacZ at the cloning site for blue/white selection. T7 & Sp6 phage promoters for transcription of inserted genes. BACs are now being
Bacterial artificial chromosome
Bacterial_artificial_chromosome
Angle between the rotational axis and orbital axis of a body
05″ t6 + 7.12″ t7 + 27.87″ t8 + 5.79″ t9 + 2.45″ t10 where here t is multiples of 10,000 Julian years from J2000.0. These expressions are for the so-called
Axial_tilt
Biological process of gene regulation
interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi". Molecular and Biochemical Parasitology. 133
RNA_interference
Protein-coding gene in humans
RD, Sanderson CM (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within
POLE_(gene)
Tomato with modified genes
Zeneca used a similar technology on a commercially successful variety called T7, adding a truncated version of the PG gene. It was used to make a tomato paste
Genetically_modified_tomato
Interdisciplinary branch of biology and engineering
including the nitrogen fixation cluster and type III secretion system along with bacteriophages T7 and ΦX174. While DNA is most important for information storage
Synthetic_biology
Scientific instrument that automates the DNA sequencing process
chip via the fluidic system, and later a sequencing primer is added to the adaptor region of DNBs for hybridization. DNBSEQ-T7 can generate short reads
DNA_sequencer
Part of the vertebral column in animals
Retrieved February 6, 2008. 4000 sets of digital images, showing spatial expression patterns for various genes in adult and juvenile mouse spinal cords from
Spinal_cord
Sendai virus, virus of rodents
containing the transgene of interest, along with plasmids that encode the T7 RNA polymerase and the viral genes NP, P, F5R (a modified F protein), and
Murine_respirovirus
bones of the human skeleton List of nerves of the human body Circulatory system Blood vessel The UK English names differ mainly by the addition of dashes
List of skeletal muscles of the human body
List_of_skeletal_muscles_of_the_human_body
Short length polypeptides
micropeptide cDNA into a plasmid containing a T7 or SP6 promoter. This method utilizes a cell-free protein-synthesizing system in the presence of 35S-methionine to
Micropeptide
Compound analogous to naturally occurring RNA and DNA
artificial replication and transcription polymerases starting generally from T7 RNA polymerase; (16S ribosomal sequences with altered anti-Shine-Dalgarno
Nucleic_acid_analogue
Complete set of molecular interactions in a biological cell
Bacteriophage Escherichia coli bacteriophage lambda Escherichia coli bacteriophage T7 Streptococcus pneumoniae bacteriophage Dp-1 Streptococcus pneumoniae bacteriophage
Interactome
Protein-coding gene in the species Homo sapiens
G6b-B, G6b-D, and G6b-E were cloned into the pcDNA3, in combination with T7 epitope as a fusion which leads to fusion proteins. The new fusion proteins
G6B
Major roads in Ireland
(T6) and the T7 routes. Unlike the present system, where each road (whether N- or R-) has a unique number, under the Trunk and Link Road system, Link Roads
Trunk_roads_in_Ireland
meanings around the process of gene expression from regulatory sequences —promoters and enhancers. Gene expression has been commonly characterized as a
Promoter_activity
Antibiotic medication
recombinant protein expression in bacteria. RNA encoding for the recombinant gene is usually transcribed from DNA by a viral T7 RNA polymerase, which
Rifampicin
Enzyme that synthesizes RNA from DNA
Perhaps the most widely studied such single-subunit RNAP is bacteriophage T7 RNA polymerase. ssRNAPs cannot proofread. B. subtilis prophage SPβ uses YonO
RNA_polymerase
Chemical compound
(January 2016). "Isolating dividing neural and brain tumour cells for gene expression profiling". Journal of Neuroscience Methods. 257: 121–133. doi:10.1016/j
5-Ethynyl-2'-deoxyuridine
American robotics company
news.harvard.edu. 20 June 2019. Retrieved February 22, 2021. "RLA-Y1 Terra T7 Robot Mower by iRobot". Retrieved 2 September 2019. "Robot Lawn Mower". www
IRobot
phones Countdown Countdowns usually start at ten, nine... T8 Tetris Imitative T7 Bowling Hand The difficult Seven-ten split Daniel Negreanu (10♠ 7♠) His favorite
List of poker playing card nicknames
List_of_poker_playing_card_nicknames
Study of lichen taxonomy and evolution
state. During wet-drying cycles, the microbial community shifts gene expression. In wet conditions, genes for nutrient transport and metabolism in bacteria
Lichen_systematics
Temperature-dependent RNA structure
a temperature-sensitive non-coding RNA molecule which regulates gene expression. Its unique characteristic it is that it does not need proteins or metabolites
RNA_thermometer
Class of enzymes that unpack genetic material
have a RecA fold. The most studied SF4 helicase is gp4 from bacteriophage T7. Superfamily 5 (SF5): Rho proteins conform the SF5 group. They have a RecA
Helicase
Type of vaccine
promoter and sequence which corresponds to the mRNA construct. By combining T7 phage RNA polymerase and the plasmid DNA, the mRNA can be transcribed in the
MRNA_vaccine
Season of television series
player Kim was assigned to the boys' group while the girls got Francis. ^T7: The housemates only opened 98 out of 100 locks. The housemate first mentioned
Pinoy Big Brother: Teen Edition 4
Pinoy_Big_Brother:_Teen_Edition_4
Type of RNA molecules
functionality in comparison to copying of the same template by proteins such as the T7 RNA polymerase. An RPR called t5(+1) adds triplet nucleotides at a time instead
Ribozyme
Science of synthetic life forms
reassignment of UAG translation function. The GRO exhibited increased resistance to T7 bacteriophage, thus showing that alternative genetic codes do reduce genetic
Xenobiology
Chemical compound
1038/s41467-023-39151-8. PMC 10272112. PMID 37322067. Jiang, Y; Cronan, JE (2005). "Expression cloning and demonstration of Enterococcus faecalis lipoamidase (pyruvate
Lipoic_acid
& Dunn, J. J. (1986). "Synthesis of Infectious Poliovirus RNA by Purified T7 RNA Polymerase". Proc. Natl. Acad. Sci. U.S.A. 83 (8): 2330–2334. doi:10.1073/pnas
Eckard_Wimmer
American geneticists
offspring. Her research uses Caenorhabditis elegans, a worm, as a model system. Li, S. (2004-01-23). "A Map of the Interactome Network of the Metazoan
Susan_Strome
Language family spoken in Mesoamerica
that ejective /tʼ/ and the non-ejective sequence /tʔ/ (previously tʼ and t7) are both written tʼ. Other major Maya languages, primarily in the Mexican
Mayan_languages
DNA polymerases that originate from thermophiles
while others like the Bst Klenow fragment are thermostable. The T4, T6 and T7 DNA polymerases are also not thermostable. The standard reverse transcriptases
Thermostable_DNA_polymerase
Anticonvulsant medication
RJ, Li JY, Nagaya M, Zhang C, Pardridge WM (October 1999). "Selective expression of the large neutral amino acid transporter at the blood-brain barrier"
Pregabalin
suPARnostic - supercoil - SurE, survival protein E - Syb-prII-1 - syndrome - T7 RNA polymerase - taq polymerase - TATA box - taurochenodeoxycholate 6α-hydroxylase
Index of molecular biology articles
Index_of_molecular_biology_articles
generated by the original PCR step undergoes in vitro transcription using the T7 promoter incorporated in the ad1 primer to ensure RNA transcripts come from
G&T-seq
Various uses and techniques of polymerase chain reaction in the laboratory
fidelity of replication than the Klenow fragment, but is also destroyed by heat. T7 DNA polymerase (family B) has similar properties and purposes. It has been
Variants_of_PCR
Modified genetic code
specific cellular components. Producing better protein: the evolution of T7 bacteriophages on a non-evolving E. coli strain that encoded 3-iodotyrosine
Expanded_genetic_code
Dopamine agonist medication
allodynia from sciatic nerve damage in mice, and alter IL-1β and IL-10 expression from immune cell culture". Neuroscience Letters. 814 137419. doi:10.1016/j
Pramipexole
Federal region of Belgium including the capital
region. As of 2017[update], the Brussels tram system consists of 17 tram lines (three of which – lines T4, T7 and T10 – qualify as premetro lines that partly
Brussels
Gram-negative gammaproteobacterium
was by Delbrück and Luria in 1942 in their study of bacteriophages T1 and T7. The original E. coli B strain, known then as Bacillus coli, originated from
Escherichia coli in molecular biology
Escherichia_coli_in_molecular_biology
Class of tank
Tank Mk I, was the first Infantry tank (I tank) and the first practical expression of the decision to split design into I tanks and cruiser tanks, with different
Infantry_tank
DNA replication in eukaryotic organisms
Washington MT, Moore KC, Patel SS (May 1997). "The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F1-ATPase". Proceedings
Eukaryotic_DNA_replication
Classification of tank
the Germans and eventually adopted by other nations, found their best expression in formations of mutually-supporting medium tanks and motorised infantry
Medium_tank
City in Peru
tolerant stance toward the free-thinking tendencies of Arequipans. One expression was the creation of the Academia Lauretana of Sciences and Arts on 10
Arequipa
Broad-spectrum antibiotic
2016, a synthetic peptide-conjugated PMO (PPMO) was found to inhibit the expression of New Delhi metallo-beta-lactamase 1, an enzyme that many drug-resistant
Meropenem
Mutation that shifts codon alignment
(MIDT) of GTC GTT C TTA CAA (MIDC) to study frameshifts. E. coli pol I Kf and T7 DNA polymerase mutant enzymes devoid of 3′ → 5′ exonuclease activity produce
Frameshift_mutation
Genus of Gram-negative bacteria
aeruginosa Phages LKD16 and LKA1: Establishment of the φKMV Subgroup within the T7 Supergroup". Journal of Bacteriology. 188 (19): 6924–6931. doi:10.1128/JB
Pseudomonas
Protein-coding gene in the species Homo sapiens
beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was
B4GALT5
City in France
Tram T6 from Debourg, in the south to Hôpitaux Est-Pinel in the east. Tram T7 from Vaux-en-Velin la soie, in the north-east to Décines – OL Vallée in the
Lyon
There are two subsegments of the peptide that are active; T3 peptide and T7 peptide. The structure is very similar to that of endostatin, which is cleaved
Tumstatin
and biochemistry of bacteriophage T7; and for his innovative techniques for high-throughput analysis of DNA, RNA, and T7 proteins." Howard M. Temin, PhD
List of California Institute of Technology people
List_of_California_Institute_of_Technology_people
Enzymes which cleave a nucleotide chain
endoribonuclease, play a crucial role in RNA processing, a fundamental step in gene expression. This process involves the precise cleavage of precursor RNA molecules
Endonuclease
Design and manufacture of artificial nucleic acid structures
upon opening. In another example, a DNA origami nanostructure was coupled to T7 RNA polymerase and could thus be operated as a chemical energy-driven motor
DNA_nanotechnology
Biological process
which stimulate expression of genes that encode components of the DNA synthetic machinery. G1/S-Cdk activation also promotes the expression and activation
DNA_replication
Representation of the symmetry group of spacetime in special relativity
holomorphic and anti-holomorphic respectively. Closer examination of the expression (S8) also allows for identification of π + {\displaystyle \pi ^{+}} and
Representation theory of the Lorentz group
Representation_theory_of_the_Lorentz_group
Chemical compound
II, but not group I, mGluRs in the rat nucleus accumbens inhibits the expression of conditioned hyperactivity in an amphetamine-associated environment"
EGLU
Extrapolation method to detect common ancestors
experimental evolutionary study in which replicate populations of bacteriophage T7 were propagated to generate an artificial phylogeny. In revisiting these experimental
Ancestral_reconstruction
Pseudoscientific personality theory
Vasilyev, V.N., Medvedev, M.A. et al. Physiological and Psychological Expression of Personality Types. Human Physiology 29, 729–734 (2003). https://doi
Socionics
Historic site in Queensland, Australia
(plan adjusted November 1957). A combination of Type F/T5 (upper floor) and F/T7 (lower floor), according to Burmester et al, Queensland Schools: A Heritage
Indooroopilly State High School Buildings
Indooroopilly_State_High_School_Buildings
Type of mycotoxin
that nivalenol can change the expression of cytokines, which are important controller molecules of the immune system. Nivalenol induced the secretion
Nivalenol
Methodic assignment of colors to elements of a graph
generalization of the chromatic polynomial, the Tutte polynomial. These expressions give rise to a recursive procedure called the deletion–contraction algorithm
Graph_coloring
Sequence in a genome
origins in single cells in metazoan systems and correlating these initiation events with single-cell gene expression and chromatin status will be important
Origin_of_replication
Chemical compound
involved in the regulation of non-histaminergic systems. A proposed mechanism involves the enhanced expression of neuropeptide Y (NPY) rather than HDC inhibition
Α-Fluoromethylhistidine
Study of the effects of temperature on biology
green fluorescent protein gene; the gene fusion was then transcribed from the T7 promoter in E. coli, and fluorescence was observed at 37 °C but not at 30 °C
Thermobiology
Experimental process
analog into a unique site. This can be done by transcribing a short RNA using T7 RNA polymerase, then synthesizing a short oligonucleotide containing the analog
Nucleic acid structure determination
Nucleic_acid_structure_determination
T7 EXPRESSION-SYSTEM
T7 EXPRESSION-SYSTEM
Boy/Male
Arabic, Muslim, Pashtun
Expression
Boy/Male
Tamil
Expression
Boy/Male
Celebrity, Gujarati, Hindu, Indian, Kannada, Telugu
Art of Expressing Acting; Expression
Girl/Female
Muslim/Islamic
Grace Expression
Girl/Female
Tamil
Cheerful expression
Girl/Female
Hindu, Indian
Expressions
Boy/Male
Arabic, Muslim, Sindhi
Expression; Countenance; Border
Girl/Female
Indian
Face, Warm expression
Boy/Male
Indian, Sanskrit
Expression
Boy/Male
Muslim
Expression
Girl/Female
Tamil
Expressions
Girl/Female
Indian
Expressions
Girl/Female
Indian
Cheerful expression
Boy/Male
Arabic, Bengali, Biblical, Hindu, Indian, Kannada, Marathi, Muslim, Sindhi
Submission; Clearness; Expression
Girl/Female
Tamil
Abbynayha | அபà¯à®ªà¯à®¯à¯à®¨à®¾à®¯à¯à®¹à®¾
Expressions
Abbynayha | அபà¯à®ªà¯à®¯à¯à®¨à®¾à®¯à¯à®¹à®¾
Girl/Female
Indian
Expressions
Boy/Male
Indian
Expression
Girl/Female
Tamil
Expression
Boy/Male
Tamil
Abhihita | அபிஹீதாÂ
Expression, Word, Name
Abhihita | அபிஹீதாÂ
Girl/Female
Sikh
Expression
T7 EXPRESSION-SYSTEM
T7 EXPRESSION-SYSTEM
Biblical
fruit; fruitful
Surname or Lastname
English
English : variant spelling of Read.
Boy/Male
Hindu, Indian
Sword
Boy/Male
Hindu, Indian
Lord Krishna
Girl/Female
Arabic, Muslim
Branch; Twig; Plural of Ghusn
Boy/Male
British, English, Jamaican
Blond; From the White Lock of Hair
Girl/Female
Hindu, Indian
Increase
Girl/Female
Arabic, Muslim
Beauty; Pretty
Girl/Female
Finnish, Indian
To Pour Water to God; Wisp
Girl/Female
Russian
Hope.
T7 EXPRESSION-SYSTEM
T7 EXPRESSION-SYSTEM
T7 EXPRESSION-SYSTEM
T7 EXPRESSION-SYSTEM
T7 EXPRESSION-SYSTEM
n.
Wrong expression.
a.
Of, or relating to, expression; phraseological; also, vividly representing or suggesting an idea or sentiment.
n.
A form of words in which an idea or sentiment is conveyed; a mode of speech; a phrase; as, a common expression; an odd expression.
n.
The act of declaring or signifying; declaration; utterance; as, an expression of the public will.
n.
The pressure of the type on the paper, or the result of such pressure, as regards its appearance; as, a heavy impression; a clear, or a poor, impression; also, a single copy as the result of printing, or the whole edition printed at a given time.
n.
The act or manner of expressing in words; style of expression; phrasing.
n.
A sense of heaviness or obstruction in the body or mind; depression; dullness; lassitude; as, an oppression of spirits; an oppression of the lungs.
pl.
of Expressman
n.
The representation of any quantity by its appropriate characters or signs.
n.
Lively or vivid representation of meaning, sentiment, or feeling, etc.; significant and impressive indication, whether by language, appearance, or gesture; that manner or style which gives life and suggestive force to ideas and sentiments; as, he reads with expression; her performance on the piano has expression.
n.
The act of expressing; expression; utterance; representation.
a.
Without expression or meaning; not expressive; dull; unintelligent; as, an inexpressive countenance.
a.
Full of expression; vividly representing the meaning or feeling meant to be conveyed; significant; emphatic; as, expressive looks or words.
a.
Destitute of expression.
a.
With expression.
n.
The act of repressing, or state of being repressed; as, the repression of evil and evil doers.
n.
That which is expressed by a countenance, a posture, a work of art, etc.; look, as indicative of thought or feeling.
n.
Term; expression; phrase.
n.
The act of expressing; the act of forcing out by pressure; as, the expression of juices or oils; also, of extorting or eliciting; as, a forcible expression of truth.
a.
Serving to express, utter, or represent; indicative; communicative; -- followed by of; as, words expressive of his gratitude.