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Supravital staining is a method of staining used in microscopy to examine living cells that have been removed from an organism. It differs from intravital
Supravital_staining
Technique used to enhance visual contrast of specimens observed under a microscope
living cell, when supravital stains enter a living cell, they might produce a characteristic pattern of staining different from the staining of an already
Staining
Stain that doesn't kill living cells when applied
of medical specialties. In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise
Vital_stain
Abnormal inclusions in red blood cells
hemoglobin. They are not visible with routine blood staining techniques, but can be seen with supravital staining. The presence of Heinz bodies represents damage
Heinz_body
Blue dye also used as a medication
the staining of nucleoli and polychromatophilic RBCs (reticulocytes). A traditional application of methylene blue is the intravital or supravital staining
Methylene_blue
Soluble chemical substance or natural material which can impart color to other materials
the term "vital staining" means the polar opposite of "supravital staining." If living cells absorb the stain during supravital staining, they exclude it
Dye
Chemical compound
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. It is classified as an oxazine dye. N95 dust masks, eye shields, and gloves
Brilliant_cresyl_blue
Chemical compound
many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood. It can be used for staining the
Neutral_red
Immature red blood cell
slightly bluer than other red cells when looked at with the normal Romanowsky stain. Reticulocytes are also relatively large, a characteristic that is described
Reticulocyte
Substance used as a blue dye and as a medication
histopathology, typically for staining immature red blood cells. It is a supravital stain. It is closely related to methylene blue, an older stain in wide use. New
New_methylene_blue
Fluorescent dye used to stain DNA
cell-permeable and can bind to DNA in live or fixed cells. Thus, these stains are often called supravital, meaning that live cells survive a treatment with these compounds
Hoechst_stain
Pharmaceutical drug
While Heinz bodies are not visible on a Giemsa-stained smear, they can be detected with supravital staining, appearing as 1- to 3-μm inclusions in RBCs (inset)
Rasburicase
Topics referred to by the same term
Benzocyclobutene, a polycyclic aromatic hydrocarbon Brilliant cresyl blue, a supravital stain Bangladesh Cricket Board, the main governing body for cricket in Bangladesh
BCB
Aggregates of specific proteins in neurons and tissues
refractile inclusions not visible on a Wright's stain film. They are best identified by supravital staining with basic dyes. Hemoglobin H inclusions – alpha
Inclusion_bodies
Organelle in eukaryotic cells responsible for respiration
"granule". Leonor Michaelis discovered that Janus green can be used as a supravital stain for mitochondria in 1900. In 1904, Friedrich Meves made the first recorded
Mitochondria
German chemist and physician (1875–1949)
he worked extensively on quinones, and discovered Janus green as a supravital stain for mitochondria and the Michaelis–Gutmann body in urinary tract infections
Leonor_Michaelis
Medical condition
Vdovenko AA, Williams JE (2000). "Blastocystis hominis: neutral red supravital staining and its application to in vitro drug sensitivity testing". Parasitol
Blastocystosis
American zoologist
Newspapers.com. Worley, L. G.; Worley, E. K. (1943). "Studies of the Supravitally Stained Golgi Apparatus". Journal of Morphology. 73 (2): 365–399. doi:10
Elizabeth_K._Worley
Chemical compound
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis
Janus_Green_B
Cell membrane organelle
Zbigniew (1994). "Chapter 12 Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations".
Lysosome
Medical laboratory instrument
like their manual counterparts, employ the use of a supravital dye such as new methylene blue to stain the red cells containing reticulin prior to counting
Automated_analyser
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SUPRAVITAL STAINING
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SUPRAVITAL STAINING
n.
Painting or staining, in imitation of the grain of wood, atone, etc.
v. t.
To change or alter the hue or tint of, by dyeing, staining, painting, etc.; to dye; to tinge; to paint; to stain.
n.
The act of painting or staining, especially of painting the face.
a.
Wanting in honor; not honorable; bringing or deserving dishonor; staining the character, and lessening the reputation; shameful; disgraceful; base.
p. pr. & vb. n.
of Stain
n.
A stain or staining; state of being in dishonor or condemnation.
n.
A brownish purple pigment, obtained by the action of some compounds of tin upon certain salts of gold. It is used in painting and staining porcelain and glass to give a beautiful purple color. Commonly called Purple of Cassius.
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