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Alternative to the polymerase chain reaction
Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). By adding a reverse transcriptase
Recombinase polymerase amplification
Recombinase_polymerase_amplification
DNA polymerases that originate from thermophiles
DNA polymerases used in isothermal DNA amplification, e.g. in loop-mediated isothermal amplification, multidisplacement amplification, recombinase polymerase
Thermostable_DNA_polymerase
Various uses and techniques of polymerase chain reaction in the laboratory
displacement amplification (SDA) are isothermal, replicating DNA at a constant temperature using a polymerase and nicking enzyme. Recombinase Polymerase Amplification
Variants_of_PCR
Medical condition
amplification-refractory mutation system (ARMS) and allele-specific recombinase polymerase amplification. These tests can identify subtypes of sickle cell disease
Sickle_cell_disease
diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method". The Journal of General Virology. 99 (8): 1012–1026
NASBA_(molecular_biology)
Extremely small diamonds used for their thermal, mechanical and optoelectronic properties
the signal from background autofluorescence. Combined with recombinase polymerase amplification, nanodiamonds enable single-copy detection of HIV-1 RNA on
Nanodiamond
Topics referred to by the same term
piloted aircraft Replication protein A Recombinase polymerase amplification, isothermal alternative to the polymerase chain reaction (PCR) Resolvable Private
RPA
Protein found in humans
2013). "An aptamer-based bio-barcode assay with isothermal recombinase polymerase amplification for cytochrome-c detection and anti-cancer drug screening"
Cytochrome_c
UK & US manufacturer of diagnostic tests
isothermal amplification with technologies such as Loop-mediated isothermal amplification (LAMP) or Recombinase polymerase amplification (RPA), for example
Abingdon_Health
tests are under trial: Diagnostics-in-a-Suitcase, based on recombinase polymerase amplification (RPA). The new equipment, about the size of a laptop and
Research in management of Ebola
Research_in_management_of_Ebola
Type of CRISPR tool
Polymerase for transcription of the gene(s) targeted by the system. In order for a protein to be made from the gene that encodes it, RNA polymerase must
CRISPR_activation
Virus of the herpes family
sensitive detection of Epstein–Barr virus DNA in blood by recombinase aided amplification combined with magnetic beads enrichment". Journal of Virological
Epstein–Barr_virus
American scientist
tests that use several technologies, such as ELISA, Recombinase Polymerase Amplification (RPA), Polymerase Chain Reaction (PCR), nucleic acid hybridization
Chester_L._Sutula
Plant molecular biologist
post-transformation excision of marker genes using phage site-specific recombinases. The Maliga team constructed the pPZP Agrobacterium binary vector family
Pal_Maliga
Lab technique
GATCs are amplified in the subsequent PCR. A PCR with primers matching the adaptors is then carried out, leading to the specific amplification of genomic
DNA adenine methyltransferase identification
DNA_adenine_methyltransferase_identification
molecules. Recombinant DNA technology is widely used in genetic engineering. recombinase Any of a class of enzymes united by their ability to catalyze some form
Glossary of cellular and molecular biology (M–Z)
Glossary_of_cellular_and_molecular_biology_(M–Z)
Science competition for students in Canada
"Microfluidic Point-of-Care Device for Diagnosis of HIV via Recombinase Polymerase Amplification." A novel method of HIV detection in developing countries
Sanofi_Biogenius_Canada
Model of DNA repair in biology
DSB are processed into ssDNA with 3' extensions, which allows RAD51 recombinase (eukaryotic homologue of prokaryotic RecA) to bind to it to form a nucleoprotein
Double-strand break repair model
Double-strand_break_repair_model
Methods used to change the DNA of organisms
replicating DNA sequences. Polymerase chain reaction (PCR), developed by Kary Mullis in 1983, allowed small sections of DNA to be amplified (replicated) and aided
Genetic engineering techniques
Genetic_engineering_techniques
Collection of the total genomic DNA from a single organism
vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis. There
Genomic_library
Molecular biology techniques
by enzymatic ligation, they are amplified via rolling circle amplification using a polymerase. After the amplification reaction, several-hundredfold replication
Methods to investigate protein–protein interactions
Methods_to_investigate_protein–protein_interactions
Realm of viruses
techniques that only capture dsDNA viruses. Once rolling circle amplification was implemented to amplify ssDNA genomes, a broader scope of ssDNA virus distribution
Volvereviria
Genome manipulation method
3' overhangs on the linear DNA. Subsequently, λ beta, a member of a recombinase family, binds to the 3' overhangs and mediates annealing to the complementary
No-SCAR_genome_editing
Alteration in the nucleotide sequence of a genome
expression seen during stages of development in model organisms. DNA Recombinase systems like Cre-Lox recombination used in association with promoters
Mutation
of an amplification reaction. The term is most frequently used to describe the numerous copied fragments that are the products of the polymerase chain
Glossary of cellular and molecular biology (0–L)
Glossary_of_cellular_and_molecular_biology_(0–L)
Protein found in humans
referred to as class 1, or a 23 bp spacer, referred to as class 2. A recombinase made up of RAG1 and RAG2 subunits always cleave between these two sites
DNA_repair_protein_XRCC4
Biological process of gene regulation
S2CID 18460980. Tiscornia G, Tergaonkar V, Galimi F, Verma IM (May 2004). "CRE recombinase-inducible RNA interference mediated by lentiviral vectors". Proceedings
RNA_interference
Animal that has been genetically modified
Kumar JP (2017). "FLPing Genes on and off in Drosophila". Site-Specific Recombinases. Methods in Molecular Biology. Vol. 1642. pp. 195–209. doi:10.1007/978-1-4939-7169-5_13
Genetically_modified_animal
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION
RECOMBINASE POLYMERASE-AMPLIFICATION