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The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying fluorescence microscopy. Experimental
GFP-cDNA
Protein that exhibits bright green fluorescence when exposed to ultraviolet light
fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers
Green_fluorescent_protein
American molecular biologist
almost complete gfp cDNA, with 965 bases out of the 1,050 bases of the corresponding mRNA. It would require construction of another cDNA library during
Douglas_Prasher
pH-sensitive GFPs are often used as part of a noninvasive and effective technique. By using cDNA as a template along with the appropriate primers, the GFP gene
Intracellular_pH
Species of lancelet
Mushegian, A.; Sanders, E.; Schwartz, J.; Ron Yu, C. (2008). "A family of GFP-like proteins with different spectral properties in lancelet Branchiostoma
Branchiostoma_floridae
Protein created by joining other proteins into a single polypeptide
interactions in real time. The first fluorescent tag, green fluorescent protein (GFP), was isolated from Aequorea victoria and is still used frequently in modern
Fusion_protein
Protein-coding gene in the species Homo sapiens
Typically, the EGFP or GFP tagging has been done in the N-terminus or C-terminus of the mature peptide sequence of relevant subunit. The GFP tagging of δ-subunit
GABRD
acids long and is a 28.8 kDa monomer. Dronpa is 76% similar in sequence to GFP and shares a similar structure with an 11 stranded β-barrel (a β-can) enclosing
Dronpa
protein localisation HomoloGene, compares proteins from different species gfp-cdna, protein localisation with fluorescence microscopy International Protein
Bioinformatic_Harvester
Technology used to validate protein interactions
protein (GFP) to be reassembled using an anti-parallel leucine zipper in E. coli cells. This was achieved by dissecting GFP into C- and N-terminal GFP fragments
Bimolecular fluorescence complementation
Bimolecular_fluorescence_complementation
Experimental medical treatment
protein (GFP) gene in the cones of gerbils. The genetic insert was designed to only be expressed in S or M cones, and the expression of GFP in vivo was
Gene therapy for color blindness
Gene_therapy_for_color_blindness
Scientific method
proteins as reporters, including the green fluorescent protein GFP and its variants (eGFP, YFP, CFP). Of the fluorescent reporters, calcium indicator systems
Calcium_imaging
Fluorescent protein
non-photoacivated protein. This is superior to other fluorescent proteins such as PA-GFP and KFP1, which only show low fluorescence before photoactivation. In addition
Kaede_(protein)
Method which allows the purification and detection of proteins
alkaline phosphatase (AP)) or fluorescence (e.g. green fluorescent protein (GFP)) marker. If high purity is required, the lysate can be purified by first
Strep-tag
Mammalian protein
pancreas. In 1996, Danny Welch's lab in Hershey, Pennsylvania, isolated a cDNA from a cancer cell that was not able to undergo metastasis after the human
Kisspeptin
Process of copying a segment of DNA into RNA
fusion of GFP and the MS2 coat protein, which has a high affinity, sequence-specific interaction with the MS2 stem loops. The recruitment of GFP to the site
Transcription_(biology)
Photoactivatable fluorescent protein
to the green fluorescent protein (GFP), in recent years many other fluorescent proteins have been cloned. Unlike GFPs, which are derived from the luminescent
Eos_(protein)
Arrangement of amino acid sequence
fluorescent protein (GFP) allowed researchers to measure the activity of calmodulin via the fluorescence of the split-GFP. Regions of GFP that tolerate the
Circular permutation in proteins
Circular_permutation_in_proteins
Protein-coding gene in the species Homo sapiens
NKX3-1 expression was visualized using Fluorescence microscopy, utilizing GFP-NKX3-1 in the nucleus. NKX3-1 expression acts as a transcription factor that
NKX3-1
first PAFP, Kaede (protein), was isolated from Trachyphyllia geoffroyi in a cDNA library screen designed to identify new fluorescent proteins. A fluorescent
Photoactivatable fluorescent protein
Photoactivatable_fluorescent_protein
Antifungal peptide
B, Uttenweiler-Joseph S, Michaut L, et al. (August 1998). "A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is
Drosomycin
Database of nematode genomics
expression profiles (stage, tissue and cell) from microarrays, SAGE analysis and GFP promoter fusions; The complete cell lineage of the worm; The wiring diagram
WormBase
Chemical compound
forming efficiency, proliferation and K15 expression of isolated human K15-GFP+ cells in vitro." Spermidine is an aliphatic polyamine. In plants and some
Spermidine
Structures in cell nuclei
novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin". The Journal of Experimental Medicine. 173 (6): 1407–1419
Cajal_body
Protein found in humans
actively growing pluripotent adult stem cells. Green fluorescent protein (GFP), whose expression is driven by the nestin regulatory element in transgenic
Nestin_(protein)
Protein found in humans
chromosome 14. To study CBs, coilin can be combined with GFP (Green Fluorescent Protein) to form Coilin-GFP hybrid protein. The hybrid protein can then be used
Coilin
Small piece of maintainable DNA
partners that may be used for screening are the green fluorescent protein (GFP) and luciferase. A cloning vector need not contain suitable elements for
Cloning_vector
Medications based on ribonucleic acids
(August 2014). "A G-quadruplex-containing RNA activates fluorescence in a GFP-like fluorophore". Nature Chemical Biology. 10 (8): 686–91. doi:10.1038/nchembio
RNA_therapeutics
Genus of viruses
Rudin CM (2012). "Characterization of a full-length infectious cDNA clone and a GFP reporter derivative of the oncolytic picornavirus SVV-001". Journal
Senecavirus
Enzyme that creates mutations in DNA
PMID 11823785. S2CID 4349496. Rada C, Jarvis JM, Milstein C (May 2002). "AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of
Activation-induced cytidine deaminase
Activation-induced_cytidine_deaminase
Protein-coding gene in the species Homo sapiens
characteristic for the HMG family comprising HMGA, HMGB and HMGN proteins. HMGA1-GFP fusion proteins are highly dynamic in vivo (determined using FRAP analysis)
HMGA1
Protein-coding gene in the species Homo sapiens
gamma-actin colocalizes with alpha-actinin and GFP-labeled gamma actin localized to Z-discs, whereas GFP-alpha-actin localized to pointed ends of thin
Actin,_cytoplasmic_2
G protein
degenerate oligonucleotide primers were synthesized and mixed with a taste tissue cDNA library. The DNA products were amplified by the polymerase chain reaction
Gustducin
Molecular biology technique
bait and prey proteins interact and bring the fluorescent proteins (FP1 = GFP, FP2=mCherry) in close proximity at the binding site of the LacI protein
Two-hybrid_screening
Protein-coding gene in the species Homo sapiens
PMID 10591637. S2CID 33506220. He W, Ikeda S, Bronson RT, et al. (2001). "GFP-tagged expression and immunohistochemical studies to determine the subcellular
TUB_(gene)
Type of virus
CM (December 2012). "Characterization of a full-length infectious cDNA clone and a GFP reporter derivative of the oncolytic picornavirus SVV-001". The Journal
Oncolytic_virus
Awards for Philippines-resident Filipino-citizens
University Design and Construction of a Synthetic Coconut Cadang Cadang Viroid cDNA Marvin C. Masalunga B.S. Biology University of the Philippines Diliman Genetic
BPI-DOST_Science_Awards
Transcription factor gene involved in the urogenital system
examine the expression levels and localisation of WT1, a mouse model using WT1-GFP (green fluorescent protein) knock-in has been made. This model showed, that
Wilms_tumor_protein
Protein-coding gene in the species Homo sapiens
(2010). "Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins". Mol Biol Rep. 37 (3): 1165–8. doi:10.1007/s11033-009-9477-7
NUBP2
Field of molecular biology
promoter and reporter gene (such as luciferase or green fluorescent protein (GFP). These plasmids are transfected into cells and incubated for a certain period
Functional_genomics
Protein-coding gene in the species Homo sapiens
originally identified through sequence mutations and translational fusions with gfp (green fluorescent protein) and remain to be confirmed. RFX1 has been shown
RFX1
Protein found in humans
CRE-luciferase reporter system. Results: An anti-GFP antibody recognized the hTAAR1 fusion proteins only when GFP was at the N-terminus, but not the C-terminus
TAAR1
Laboratory technique in biochemistry
detailed above. Examples of tags in use are the green fluorescent protein (GFP) tag, glutathione-S-transferase (GST) tag and the FLAG-tag tag. While the
Immunoprecipitation
Use of baculovirus to deliver genes to mammalian cells
Characterization of serotonin receptor activation via a BacMam delivered GFP fusion to a kinase substrate has been performed. The BacMam system can be
BacMam
Conversion of a gene's sequence into a mature gene product or products
imaging using a fluorescence microscope. It is very difficult to clone a GFP-fused protein into its native location in the genome without affecting expression
Gene_expression
Steglich C (January 2010). "Seed-based INTARNA prediction combined with GFP-reporter system identifies mRNA targets of the small RNA Yfr1". Bioinformatics
List of RNA structure prediction software
List_of_RNA_structure_prediction_software
Protein-coding gene in humans
(March 2000). "Analysis of beta-catenin aggregation and localization using GFP fusion proteins: nuclear import of alpha-catenin by the beta-catenin/Tcf
TCF7L2
Protein family
outside of the fungi. Membrane fractionation and green fluorescent protein (GFP) fusion studies established that Alr1p is localised to the plasma membrane
Magnesium_transporter
Treatment for blindness
example, in rats the murine rhodopsin gene drive the expression in AAV2, GFP reporter product was found only in rat photoreceptors, not in any other retinal
Gene therapy of the human retina
Gene_therapy_of_the_human_retina
Sendai virus, virus of rodents
also be infected and also achieve 100% GFP positivity. In fibroblasts, an intracellular virus-associated GFP expression was stable at least for more
Murine_respirovirus
upstream (i.e. -75) of the transcription start site for many eukaryotic genes. cDNA See complementary DNA. cell The basic structural and functional unit of which
Glossary of cellular and molecular biology (0–L)
Glossary_of_cellular_and_molecular_biology_(0–L)
Protein-coding gene in the species Homo sapiens
When Myo10 was tagged with Green Fluorescent Protein (GFP) and expressed in cells, small puncta of GFP-Myo10 were observed moving forward within filopodia
MYO10
Protein-coding gene in the species Homo sapiens
FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET)". J. Cell. Biochem
FHL2
GFP CDNA
GFP CDNA
Girl/Female
Arabic, Indian, Muslim, Punjabi, Sikh
One who Meets; One with Strong Intentions; One with Decisiveness; Distance; Gap
Girl/Female
Indian, Telugu
Goddess Lakshmi
Girl/Female
Indian
Who bridges the gap
Surname or Lastname
English (West Yorkshire)
English (West Yorkshire) : topographic name for someone who lived by a gap between hills, from Middle English sherd, sharde (Old English sceard, a derivative of sceran ‘to cut or shear’).
Boy/Male
Native American
Where the wind blows down the gap.
Surname or Lastname
English
English : from Middle English gappe, Old Norse gap ‘chasm’, ‘breach’, hence a topographic name for someone who lived near a gap in a wall, hedge, or (in Norfolk and Suffolk) cliffs.German : from the personal name Gabo, a short form of Gebolf (see Gebhardt).
Girl/Female
Bengali, Gujarati, Hindu, Indian, Kannada, Malayalam, Marathi, Sanskrit, Tamil, Telugu
Connected; Following; Who Bridges the Gap; Absorbed
Boy/Male
Hindu
One who bridgesth gap, Friend
Girl/Female
Tamil
Anvitha | அநà¯à®µà®¿à®¤à®¾
Who bridges the gap
Anvitha | அநà¯à®µà®¿à®¤à®¾
Boy/Male
Hindu
One who bridgesth gap, Friend
Girl/Female
Indian, Malayalam, Sanskrit, Telugu
One who Bridges the Gap; Rays of Light; Absorbed; Goddess Durga
Boy/Male
Tamil
One who bridgesth gap, Friend
Girl/Female
Tamil
Anvita | அநà¯à®µà®¿à®¤à®¾
Who bridges the gap
Anvita | அநà¯à®µà®¿à®¤à®¾
Male
Native American
Native American Hopi name APONIVI means "where the wind blows down the gap."
Girl/Female
Indian
Who bridges the gap
Boy/Male
Tamil
One who bridgesth gap, Friend
Female
English
English name derived from the Scottish place name Morvern, from Gaelic Mhorbhairne, MORVEN means "the big gap."
GFP CDNA
GFP CDNA
Girl/Female
Hindu
Queen of vrndavana
Girl/Female
Biblical
Darkened; covered; his people.
Boy/Male
Hindu, Indian, Sanskrit, Telugu
Character; Nature
Girl/Female
Arabic, Muslim
Sweet-smelling; Precious; Valuable; Fragrant
Boy/Male
English German Teutonic
From Windsor. Surname and place name. The house of Windsor has been the ruling family of the UK...
Boy/Male
Arabic African Muslim Biblical
Peace.
Girl/Female
German, Swedish
God is My Oath; My God is Bountiful; God of Plenty; God's Promise; Abbreviation of Elizabeth
Male
Irish
Irish Gaelic legend name, thought by some to have been derived from Latin Bartholomaeus, PARTHALÃN means "son of Talmai." As the legend goes, this name belonged to an early invader of Ireland who was the first to arrive on those shores after the biblical flood.
Female
Chinese
little compassionate one.
Girl/Female
Biblical
Killing, a cook.
GFP CDNA
GFP CDNA
GFP CDNA
GFP CDNA
GFP CDNA
v. t.
To notch, as a sword or knife.
n.
A void space; a gap or break, as in ranks of men.
n.
A college servant; -- so called in Cambridge, England; at Oxford called a scout.
n.
That which closes or fills up an opening or gap; hence, a temporary expedient.
v. t.
A cleft; a gap; a ravine; -- rarely used except as part of a proper name; as, Kaaterskill Clove; Stone Clove.
n.
A small cleft, rent, or fissure, of greater length than breadth; a gap or crack; as, the chinks of wall.
n.
An opening in anything made by breaking or parting; as, a gap in a fence; an opening for a passage or entrance; an opening which implies a breach or defect; a vacant space or time; a hiatus; a mountain pass.
n.
A vacant space, or gap, esp. between teeth in a jaw.
n.
A small hole or gap through which a wild animal passes; a muse.
n.
A small opening; a small pit or depression; a small blank space; a gap or vacancy; a hiatus.
v. t.
To make an opening in; to breach.
n.
A gap.
v. i.
To unclose; to form a hole, breach, or gap; to be unclosed; to be parted.
n.
A college student's or undergraduate's servant; -- so called in Oxford, England; at Cambridge called a gyp; and at Dublin, a skip.
n.
A servant. See Gyp.
a.
Having interstices between the teeth.
v. i.
To pen or part widely; to exhibit a gap, fissure, or hiatus.
n.
A gap in a fence.
n.
A place which is open; a breach; an aperture; a gap; cleft, or hole.
v. t.
To take out the entrails of (herrings).