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Chain-elongating inhibitor of DNA polymerase
Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' because
Dideoxynucleotide
Method of DNA sequencing developed in 1977
electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being
Sanger_sequencing
Any of several glycosylamines comprising a nucleobase and a sugar molecule
(LNA), morpholinos and peptide nucleic acids (PNA). In sequencing, dideoxynucleotides are used. These nucleotides possess the non-canonical sugar dideoxyribose
Nucleoside
Thermostable form of DNA polymerase I used in polymerase chain reaction
"Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation". Proceedings of the National Academy of Sciences of
Taq_polymerase
Discipline in genetics
which is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Recently, shotgun
Genomics
Biochemist
polymerase primed synthesis ("plus and minus") technique and, eventually, dideoxynucleotide chain-terminating sequencing, which was later termed Sanger sequencing
Alan_Coulson
Process of determining the nucleic acid sequence
it was based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase during in vitro DNA replication. Sanger
DNA_sequencing
Method for sequencing DNA
to or greater than that from the Sanger sequencing method based on dideoxynucleotide chain termination. The longer read length allows de novo genome sequencing
Single-molecule real-time sequencing
Single-molecule_real-time_sequencing
In genetics and biochemistry, determining the structure of an unbranched biopolymer
labeled-primer approach. This is accomplished by labelling each of the dideoxynucleotide chain-terminators with a separate fluorescent dye, which fluoresces
Sequencing
Compound analogous to naturally occurring RNA and DNA
or, for PNA, other methods of peptide synthesis.[citation needed] Dideoxynucleotides are used in sequencing. These nucleoside triphosphates possess a non-canonical
Nucleic_acid_analogue
DNA analysis method
Hybridization is followed by single-base extension with hapten-labeled dideoxynucleotides. The ddCTP and ddGTP are labeled with biotin while ddATP and ddUTP
Illumina_Methylation_Assay
Scientific instrument that automates the DNA sequencing process
cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased
DNA_sequencer
Measurement of genetic variations
approaches which use the incorporation of either fluorescently labeled dideoxynucleotides (ddNTP) or fluorescently labeled deoxynucleotides (dNTP). With ddNTPs
SNP_genotyping
Sequencing technique for non-coding RNA
terminal transferase or 'tailing' activity' to add a chain-terminating dideoxynucleotide base, ddRTP (ddATP or ddGTP), to the 3' end of the IT, in the presence
Ordered_two-template_relay
Lab procedure detecting 5-methylcytosines in DNA
methylation analysis using bisulfite-treated DNA utilized PCR and standard dideoxynucleotide DNA sequencing to directly determine the nucleotides resistant to
Bisulfite_sequencing
DNA polymerases that originate from thermophiles
polymerase can also be used for the incorporation of fluorescence-labelled dideoxynucleotides. The template specificity of the polymerases is increased by using
Thermostable_DNA_polymerase
Alan Coulson presented a rapid gene sequencing technique which uses dideoxynucleotides and gel electrophoresis. 1978 – Frederick Sanger presented the 5,386
Timeline of biology and organic chemistry
Timeline_of_biology_and_organic_chemistry
template sequence, during which fluorochrome-labeled, chain-terminating dideoxynucleotides are randomly incorporated in the elongating strand; the resulting
Glossary of cellular and molecular biology (M–Z)
Glossary_of_cellular_and_molecular_biology_(M–Z)
Enzyme
nucleotides, which accounts for the sensitivity of T7 polymerase to dideoxynucleotides (see Sequenase below). The primary gp5 subunit of T7 DNA Polymerase
T7_DNA_polymerase
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