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Artificial plasmid
pBR322 is a plasmid and was one of the first widely used cloning vectors for E. coli. Created in 1977 in the laboratory of Herbert Boyer at the University
PBR322
technique used in recombinant DNA engineering where a plasmid (such as pBR322) is used to disable the expression of a gene. The inactivation of a gene
Insertional_mutagenesis
The tetA gene is also present in the widely used E. coli cloning vector pBR322, where it is often referred to by the name of its tetracycline-resistance
TetR
Small piece of maintainable DNA
15 kb in size. One of the earliest commonly used cloning vectors is the pBR322 plasmid. Other cloning vectors include the pUC series of plasmids, and a
Cloning_vector
utilize mutated plasmids that replicate to high copy number. For example, pBR322 is a medium copy number plasmid (~20 copies/cell) from which several high
Plasmid_copy_number
Dense cluser of restriction sites in DNA
1977, scientists Francisco Bolivar and Raymond L. Rodriguez, built the pBR322 plasmid. While not being officially a multiple cloning site, this plasmid
Multiple_cloning_site
Biological particle which transfers genetic material between cells
The pBR322 plasmid is one of the first plasmids widely used as a cloning vector.
Vector_(molecular_biology)
Set of methods in molecular biology
Diagram of a commonly used cloning plasmid; pBR322. It's a circular piece of DNA 4361 bases long. Two antibiotic resistance genes are present, conferring
Molecular_cloning
Small DNA molecule within a cell
[clarification needed] In contrast, plasmids used in biotechnology, such as pUC18, pBR322 and derived vectors, hardly ever contain toxin-antitoxin addiction systems
Plasmid
Genetically engineered chromosome derived from the DNA of yeast
Schematic of the pBR322 plasmid generated in the Boyer Lab at UC San Francisco by Bolivar and Rodriguez in 1972. It is one of the first and most widely
Yeast_artificial_chromosome
DNA or RNA sequence that matches its complement when read backwards
called insertional inactivation or insertional mutagenesis. For example, in PBR322 methylation at the tetracyclin resistant gene makes the plasmid liable to
Palindromic_sequence
Enzyme
were found in some phages (bacteriophage Mu group) and plasmids (pSC101, pBR322). Recently, high throughput mapping of DNA gyrase sites in the Escherichia
DNA_gyrase
DNA structure
recognize and cleave inverted repeat sequences within plasmids pVH51 and pBR322. The inverted repeat sequences in these plasmids displayed nicks on the
Cruciform_DNA
Enzyme
5′-GGCC-3′) was made into Escherichia coli (E.coli) in the plasmid vector pBR322. The gene was extracted from a single EcoRI fragment and a single HindIII
HaeIII
Secondary cell line
pulmonary endothelium of a human male and subsequently transfected with a PBR322 based plasmid containing the coding region for SV40 in order to immortalise
HuLEC-5a
Cell line
non-cancerous adult male individual and subsequently transfected with a PBR322 based plasmid containing the coding region for SV40 in order to immortalise
MeT-5a
Ability of a cell to take up DNA
for determination of transformation efficiency in Escherichia coli are pBR322 or other similarly sized or smaller vectors, such as the pUC series of vectors
Transformation_efficiency
American professor of biology
molecule, pBR322, the first general purpose molecular cloning vector approved by the National Institute of Health Guidelines. The abbreviation, “pBR322,” refers
Raymond_L._Rodriguez
link] genetics gene expression Transcription (genetics) translation λ phage pBR322 Lodes, M. J.; Dillon, D. C.; Houghton, R. L.; Skeiky, Y. A. (2004). Expression
Expression_cloning
acyltransferase - Parkinson's disease - Partial cleavage stimulation factor domain - pBR322 - PCR - pedigree - peptide - peptide-transporting ATPase - peptide bond
Index of molecular biology articles
Index_of_molecular_biology_articles
PBR322
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Girl/Female
English American
From the wood of the royal forest. From the royal fortress meadow. A surname and place name...
Female
Polish
Short form of Polish Kazimiera, KAZIA means "commands peace."
Surname or Lastname
English
English : perhaps a variant of Pamber, a habitational name from a place in Hampshire named Pamber, from Old English penn ‘fold’, ‘enclosure’ + beorg ‘hill’.
Female
French
Diminutive form of French Laure ("laurel"), LAURETTE means "little laurel tree."
Female
African
may God save.
Boy/Male
Arabic, Indian, Muslim, Parsi
Sun; Shining Sun; Cheerful
Boy/Male
Scottish Irish
From the craggy hills.' Tor is a name for a craggy hilltop and also may refer to a watchtower.
Boy/Male
Hindu
One of the kauravas
Surname or Lastname
English
English : unexplained. Compare Henthorn.
Girl/Female
Bengali, Indian
Worship; Chapel
PBR322
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