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  • PstI
  • Providencia stuartii. PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields

  • Restriction enzyme
  • C---3' 3'---C CATGG---5' PstI Providencia stuartii 5'CTGCAG 3'GACGTC 5'---CTGCA G---3' 3'---G ACGTC---5' SacI Streptomyces achromogenes 5'GAGCTC 3'CTCGAG

  • List of restriction enzyme cutting sites: A
  • SleI, SspAI AjoI 2604 Acinetobacter johnsonii 35 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' ApiI, BloHII, BspMAI, CfrA4I, CfuII, PstI

  • List of restriction enzyme cutting sites: L–N
  • NphI, Sau3AI MhaAI Mycobacterium habana 206 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AliAJI, Asp713I, Bsp63I, CstI, Ecl37kI,

  • List of restriction enzyme cutting sites: O–R
  • YCGRG--- 5' PaeHII Pseudomonas aeruginosa 4148 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AliAJI, BspBI, BspMAI, CfuII, Ecl2zI, HalII

  • List of restriction enzyme cutting sites: C–D
  • ---CTA   TAG--- 5' CflI Cellulomonas flavigena 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AjoI, BloHII, CflI, CstI, Ecl37kI, PaePI

  • List of restriction enzyme cutting sites: G–K
  • 3' 3' ---CTTAA   G--- 5' HalII Hafnia alvei B6 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AjoI, AliAJI, Bsp63I, CfrA4I, CfuII, CstI

  • List of restriction enzyme cutting sites: Bd–Bp
  •   R--- 5' BloHII Bifidobacterium longum E194b 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AjoI, Asp713I, BsuBI, CflI, CfuII, HalII

  • List of restriction enzyme cutting sites: Bst–Bv
  • 3' ---C   YCGRG--- 5' BsuBI Bacillus subtilis 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AjoI, BloHII, CflI, CstI, Ecl37kI, HalII

  • List of restriction enzyme cutting sites: Bsp–Bss
  • FnuDII, MvnI, ThaI Bsp63I Bacillus sphaericus 63 5' CTGCAG 3' GACGTC 5' ---CTGCA   G--- 3' 3' ---G   ACGTC--- 5' AjoI, Asp713I, BloHII, MhaAI, Pfl21I, PstI

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Astrological/horoscope meaning of ctgca. ctgca means: With a Life Path 7, your numbers are (7, 16/7, 25/7, 34/7). The Life Path 7 shows that you enteredd life with a gift for investigation, analysis and careful observation. You are first-class thinkers. You evaluate situations very quickly and with amazing accuracy. Accordingly, you are thorough and complete in your work, the perfectionist who expects to be a high performance level almost to righteousness.A Life Path 7 person is a peaceful and loving soul. But keep your connection to people carefully. It's easy for you to detect deception, and recognize the people of bad faith, and avoid them. You are about to have a not so large circle of friends, but if you accept someone as a friend, it's for life. It is as if you have built a large wall around you that is hard to be penetrated. There are chances that you are a charming and refined person with great composure and a sharp mind. Nevertheless, this is exclusively for you. You're probably not a very social person. Your reservation is often called aloofness, but in reality it is not. It is simply a cover for your basic sense of insecurity. There is no need to rush, it takes time to heat new friends. Associations and organizations hold little interest for you.They really like to be alone and away from the bustle of modern life. In many ways, you'd find life better when the pace of life is less hectic. You need a lot of quiet time with your own thoughts and dreams. They hate the crowds, noise, distraction and confusion.The overwhelming strength of the number 7 is the depth of thinking and reflection that they show. They gather knowledge from virtually all sources that you find. Intellectual, scientific and are studious, you do not accept a premise until you dissected the subject and arrived at your own independent conclusion.This number is very spiritual and it is often a kind of spiritual wisdom that manifests early in age. In your internal management, you build a strong sense of intuition which you deploy when needed. If you would like a law set to itself. Whatever the spiritual position you take, whether traditional or bizarre, you keep fervently to it. Once you have decided on one thing, it is almost impossible to make you consider an alternative. Adaptability is not your style, and change is a rarity.They rely heavily on their experience and intuition, but fail to accept them if it is someone's advice. Your intuitions appear to be very accurate in the rule, and knowing this, the follow the instructions and seem to be leaders.In the most negative energy use of 7, you can very pessimistic, lackadaisical, quarrelsome and become secret. A Life Path 7 person's life does not live to the fullest and win by experiments is a difficult person because of a severe shortage of living in consideration for others. There is such a negative attitude. In fact, a very selfish and corrupt person with life path 7 can be a challenge to live an active life and be productive in society to make it work. This may be why some prefer to live alone. If you have negative characteristics, they are very difficult to get rid of because you tend to think that the world owes you a fortune for your hardwork and are not being treated fairly.

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    C: Meaning of C in the acronym ctgca means: C is a letter opened at one end. It exemplies openness, a welcoming attitude and the desire to share. This means healthy behaviours, confidence towards the outside world, positive thinking, striking, scholarstic abilities shown by an open-mindedness, lively and gregarious and triumphant. C curves aaway from the left to indicate prudence, nervousness in unfamiliar territory, tense and anxious behaviours when faced with the unknown.

    T: Meaning of T in the acronym ctgca means: T is a vertical strand crossed at the top by a horizontal strand. With the top strand balanced in the middle by the vertical strand, T shows balance and stability in its ways. It is also forgoing and spiritual. With the top strand projected up by the bottom strand, T is highly ambitious, but also kind to others as it can let others rest on its horizontal top. This makes T selfless, cooperative, womanly and dutiful. It also displays a high degree of generosity and liberalism.

    G: Meaning of G in the acronym ctgca means: G is like C, but with the bottom end growing upwards. G wants to grasp as much as it can. It shows thoughtfulness, being logical, witty, alert, stretchy, conscientious, intuitive, impetuous, reasoning and uncontrollable.

    A: Meaning of A in the acronym ctgca means: The letter A has two bars connected at a pointed edge and a cross bar holding them together. From an open end to a pointed edge signifies that all energies are trained to a point to achieve the most singularly important goal. The cross bar shows caution. Failure is avoided by stringing all required resources together. A also looks like a Pyramid with the peak as the apex of the Pyramid. Pyramids are iconic. A therefore symbolizes prominence and a desire to be recognized for ones achievements. The cross bar also represents a rung in ladder. To get to the top, one has to first step on the rung. It also means originality, a strong will power and an enterprising ability. The upper-case version consists of the two slanting sides of a triangle, crossed in the middle by a horizontal bar. It shows aspiration, the dominance to be successful, positive attitude, an optimistic world view and egotism.

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